Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide
Chitosan oligosaccharide (COS) plays an important role in the growth and development of tea plants. However, responses in tea plants trigged by COS have not been thoroughly investigated. In this study, we integrated transcriptomics and metabolomics analysis to understand the mechanisms of chitosan-i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/10/5469 |
| _version_ | 1797499263473680384 |
|---|---|
| author | Dezhong Ji Lina Ou Xiaoli Ren Xiuju Yang Yanni Tan Xia Zhou Linhong Jin |
| author_facet | Dezhong Ji Lina Ou Xiaoli Ren Xiuju Yang Yanni Tan Xia Zhou Linhong Jin |
| author_sort | Dezhong Ji |
| collection | DOAJ |
| description | Chitosan oligosaccharide (COS) plays an important role in the growth and development of tea plants. However, responses in tea plants trigged by COS have not been thoroughly investigated. In this study, we integrated transcriptomics and metabolomics analysis to understand the mechanisms of chitosan-induced tea quality improvement and growth promotion. The combined analysis revealed an obvious link between the flourishing development of the tea plant and the presence of COS. It obviously regulated the growth and development of the tea and the metabolomic process. The chlorophyll, soluble sugar, and amino acid content in the tea leaves was increased. The phytohormones, carbohydrates, and amino acid levels were zoomed-in in both transcript and metabolomics analyses compared to the control. The expression of the genes related to phytohormones transduction, carbon fixation, and amino acid metabolism during the growth and development of tea plants were significantly upregulated. Our findings indicated that alerted transcriptomic and metabolic responses occurring with the application of COS could cause efficiency in substrates in pivotal pathways and hence, elicited plant growth. |
| first_indexed | 2024-03-10T03:44:58Z |
| format | Article |
| id | doaj.art-f5ddacea6cb546679c0356ef85a7d332 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:44:58Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f5ddacea6cb546679c0356ef85a7d3322023-11-23T11:23:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012310546910.3390/ijms23105469Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan OligosaccharideDezhong Ji0Lina Ou1Xiaoli Ren2Xiuju Yang3Yanni Tan4Xia Zhou5Linhong Jin6State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaChitosan oligosaccharide (COS) plays an important role in the growth and development of tea plants. However, responses in tea plants trigged by COS have not been thoroughly investigated. In this study, we integrated transcriptomics and metabolomics analysis to understand the mechanisms of chitosan-induced tea quality improvement and growth promotion. The combined analysis revealed an obvious link between the flourishing development of the tea plant and the presence of COS. It obviously regulated the growth and development of the tea and the metabolomic process. The chlorophyll, soluble sugar, and amino acid content in the tea leaves was increased. The phytohormones, carbohydrates, and amino acid levels were zoomed-in in both transcript and metabolomics analyses compared to the control. The expression of the genes related to phytohormones transduction, carbon fixation, and amino acid metabolism during the growth and development of tea plants were significantly upregulated. Our findings indicated that alerted transcriptomic and metabolic responses occurring with the application of COS could cause efficiency in substrates in pivotal pathways and hence, elicited plant growth.https://www.mdpi.com/1422-0067/23/10/5469chitosan oligosaccharidetea plant growth and developmenttranscriptomemetabolic pathwaysmetabolism |
| spellingShingle | Dezhong Ji Lina Ou Xiaoli Ren Xiuju Yang Yanni Tan Xia Zhou Linhong Jin Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide International Journal of Molecular Sciences chitosan oligosaccharide tea plant growth and development transcriptome metabolic pathways metabolism |
| title | Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide |
| title_full | Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide |
| title_fullStr | Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide |
| title_full_unstemmed | Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide |
| title_short | Transcriptomic and Metabolomic Analysis Reveal Possible Molecular Mechanisms Regulating Tea Plant Growth Elicited by Chitosan Oligosaccharide |
| title_sort | transcriptomic and metabolomic analysis reveal possible molecular mechanisms regulating tea plant growth elicited by chitosan oligosaccharide |
| topic | chitosan oligosaccharide tea plant growth and development transcriptome metabolic pathways metabolism |
| url | https://www.mdpi.com/1422-0067/23/10/5469 |
| work_keys_str_mv | AT dezhongji transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT linaou transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT xiaoliren transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT xiujuyang transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT yannitan transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT xiazhou transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide AT linhongjin transcriptomicandmetabolomicanalysisrevealpossiblemolecularmechanismsregulatingteaplantgrowthelicitedbychitosanoligosaccharide |