In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis
This study aims to evaluate the in vitro antibacterial and anti-biofilm activities of licochalcone A on Enterococcus faecalis and to investigate the possible target genes of licochalcone A in E. faecalis. This study found that licochalcone A had antibacterial activities against E. faecalis, with the...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.970901/full |
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| author | Xiaoju Liu Xiaoju Liu Yanpeng Xiong Yanpeng Xiong Yiyi Shi Yiyi Shi Xiangbin Deng Xiangbin Deng Qiwen Deng Qiwen Deng Yansong Liu Yansong Liu Zhijian Yu Zhijian Yu Duoyun Li Duoyun Li Jinxin Zheng Jinxin Zheng Peiyu Li Peiyu Li |
| author_facet | Xiaoju Liu Xiaoju Liu Yanpeng Xiong Yanpeng Xiong Yiyi Shi Yiyi Shi Xiangbin Deng Xiangbin Deng Qiwen Deng Qiwen Deng Yansong Liu Yansong Liu Zhijian Yu Zhijian Yu Duoyun Li Duoyun Li Jinxin Zheng Jinxin Zheng Peiyu Li Peiyu Li |
| author_sort | Xiaoju Liu |
| collection | DOAJ |
| description | This study aims to evaluate the in vitro antibacterial and anti-biofilm activities of licochalcone A on Enterococcus faecalis and to investigate the possible target genes of licochalcone A in E. faecalis. This study found that licochalcone A had antibacterial activities against E. faecalis, with the MIC50 and MIC90 were 25 μM. Licochalcone A (at 4 × MIC) indicated a rapid bactericidal effect on E. faecalis planktonic cells, and killed more E. faecalis planktonic cells (at least 3-log10 cfu/ml) than vancomycin, linezolid, or ampicillin at the 2, 4, and 6 h of the time-killing test. Licochalcone A (at 10 × MIC) significantly reduced the production of E. faecalis persister cells (at least 2-log10 cfu/ml) than vancomycin, linezolid, or ampicillin at the 24, 48, 72, and 96 h of the time-killing test. Licochalcone A (at 1/4 × MIC) significantly inhibited the biofilm formation of E. faecalis. The RNA levels of biofilm formation-related genes, agg, esp, and srtA, markedly decreased when the E. faecalis isolates were treated with licochalcone A at 1/4 × MIC for 6 h. To explore the possible target genes of licochalcone A in E. faecalis, the licochalcone A non-sensitive E. faecalis clones were selected in vitro by induction of wildtype strains for about 140 days under the pressure of licochalcone A, and mutations in the possible target genes were detected by whole-genome sequencing. This study found that there were 11 nucleotide mutations leading to nonsynonymous mutations of 8 amino acids, and among these amino acid mutations, there were 3 mutations located in transcriptional regulator genes (MarR family transcriptional regulator, TetR family transcriptional regulator, and MerR family transcriptional regulator). In conclusion, this study found that licochalcone A had an antibacterial effect on E. faecalis, and significantly inhibited the biofilm formation of E. faecalis at subinhibitory concentrations. |
| first_indexed | 2024-04-11T19:14:58Z |
| format | Article |
| id | doaj.art-f5e08642179c4c8f8051396d91fdaf80 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T19:14:58Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-f5e08642179c4c8f8051396d91fdaf802022-12-22T04:07:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.970901970901In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalisXiaoju Liu0Xiaoju Liu1Yanpeng Xiong2Yanpeng Xiong3Yiyi Shi4Yiyi Shi5Xiangbin Deng6Xiangbin Deng7Qiwen Deng8Qiwen Deng9Yansong Liu10Yansong Liu11Zhijian Yu12Zhijian Yu13Duoyun Li14Duoyun Li15Jinxin Zheng16Jinxin Zheng17Peiyu Li18Peiyu Li19Department of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen, ChinaDepartment of Infectious Diseases and Shenzhen Key Lab of Endogenous Infection, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, ChinaThis study aims to evaluate the in vitro antibacterial and anti-biofilm activities of licochalcone A on Enterococcus faecalis and to investigate the possible target genes of licochalcone A in E. faecalis. This study found that licochalcone A had antibacterial activities against E. faecalis, with the MIC50 and MIC90 were 25 μM. Licochalcone A (at 4 × MIC) indicated a rapid bactericidal effect on E. faecalis planktonic cells, and killed more E. faecalis planktonic cells (at least 3-log10 cfu/ml) than vancomycin, linezolid, or ampicillin at the 2, 4, and 6 h of the time-killing test. Licochalcone A (at 10 × MIC) significantly reduced the production of E. faecalis persister cells (at least 2-log10 cfu/ml) than vancomycin, linezolid, or ampicillin at the 24, 48, 72, and 96 h of the time-killing test. Licochalcone A (at 1/4 × MIC) significantly inhibited the biofilm formation of E. faecalis. The RNA levels of biofilm formation-related genes, agg, esp, and srtA, markedly decreased when the E. faecalis isolates were treated with licochalcone A at 1/4 × MIC for 6 h. To explore the possible target genes of licochalcone A in E. faecalis, the licochalcone A non-sensitive E. faecalis clones were selected in vitro by induction of wildtype strains for about 140 days under the pressure of licochalcone A, and mutations in the possible target genes were detected by whole-genome sequencing. This study found that there were 11 nucleotide mutations leading to nonsynonymous mutations of 8 amino acids, and among these amino acid mutations, there were 3 mutations located in transcriptional regulator genes (MarR family transcriptional regulator, TetR family transcriptional regulator, and MerR family transcriptional regulator). In conclusion, this study found that licochalcone A had an antibacterial effect on E. faecalis, and significantly inhibited the biofilm formation of E. faecalis at subinhibitory concentrations.https://www.frontiersin.org/articles/10.3389/fmicb.2022.970901/fulllicochalcone AEnterococcus faecalisantibacterialpersisterbiofilm |
| spellingShingle | Xiaoju Liu Xiaoju Liu Yanpeng Xiong Yanpeng Xiong Yiyi Shi Yiyi Shi Xiangbin Deng Xiangbin Deng Qiwen Deng Qiwen Deng Yansong Liu Yansong Liu Zhijian Yu Zhijian Yu Duoyun Li Duoyun Li Jinxin Zheng Jinxin Zheng Peiyu Li Peiyu Li In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis Frontiers in Microbiology licochalcone A Enterococcus faecalis antibacterial persister biofilm |
| title | In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis |
| title_full | In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis |
| title_fullStr | In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis |
| title_full_unstemmed | In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis |
| title_short | In vitro activities of licochalcone A against planktonic cells and biofilm of Enterococcus faecalis |
| title_sort | in vitro activities of licochalcone a against planktonic cells and biofilm of enterococcus faecalis |
| topic | licochalcone A Enterococcus faecalis antibacterial persister biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.970901/full |
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