New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor
The outbreak of COVID-19, a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, is regarded as the most severe of the documented coronavirus pandemics. The measurement and monitoring of SARS-CoV-2 antibody levels by serological tests are relevant for a better ep...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202126/full |
| _version_ | 1797785620436746240 |
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| author | Janikua Nelson-Mora Diana Rubio Amairani Ventura-Martínez Luis A. González Diana Del-Rio Diana Del-Rio Yuli Aranda-López Edgar Jiménez-Díaz Edgar Jiménez-Díaz Diego Zamarrón-Hernández Diana G. Ríos-López Stephanie Aguirre Yasab Ruiz-Hernandez Aarón Cruz-Ramírez Jonás S. Barjau Miguel A. Jáurez Jehú Lopez-Aparicio Andrea Campa-Higareda Tatiana Fiordelisio Tatiana Fiordelisio Tatiana Fiordelisio |
| author_facet | Janikua Nelson-Mora Diana Rubio Amairani Ventura-Martínez Luis A. González Diana Del-Rio Diana Del-Rio Yuli Aranda-López Edgar Jiménez-Díaz Edgar Jiménez-Díaz Diego Zamarrón-Hernández Diana G. Ríos-López Stephanie Aguirre Yasab Ruiz-Hernandez Aarón Cruz-Ramírez Jonás S. Barjau Miguel A. Jáurez Jehú Lopez-Aparicio Andrea Campa-Higareda Tatiana Fiordelisio Tatiana Fiordelisio Tatiana Fiordelisio |
| author_sort | Janikua Nelson-Mora |
| collection | DOAJ |
| description | The outbreak of COVID-19, a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, is regarded as the most severe of the documented coronavirus pandemics. The measurement and monitoring of SARS-CoV-2 antibody levels by serological tests are relevant for a better epidemiological and clinical understanding of COVID-19. The aim of this work was to design a method called the SARS-CoV-2 antibody detection method (SARS-CoV-2 AbDM) for fluorescence immunodetection of anti-SARS-CoV-2 IgG and IgM on both plate and microfluidic chip. For this purpose, a system with magnetic beads that immobilize the antigen (S protein and RBD) on its surface was used to determine the presence and quantity of antibodies in a sample in a single reaction. The SARS-CoV-2 AbDM led to several advantages in the performance of the tests, such as reduced cost, possibility of performing isolated or multiple samples, potential of multiplex detection, and capacity to detect whole blood samples without losing resolution. In addition, due to the microfluidic chip in conjunction with the motorized actuated platform, the time, sample quantity, and operator intervention during the process were reduced. All these advantages suggest that the SARS-CoV-2 AbDM has the potential to be developed as a PoC that can be used as a tool for seroprevalence monitoring, allowing a better understanding of the epidemiological and clinical characteristics of COVID-19 and contributing to more effective and ethical decision-making in strategies to fight against the COVID-19 pandemic. |
| first_indexed | 2024-03-13T00:56:38Z |
| format | Article |
| id | doaj.art-f5e0c3df254e4ea0a5ae55d0139b654a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T00:56:38Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f5e0c3df254e4ea0a5ae55d0139b654a2023-07-06T19:23:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-07-011110.3389/fbioe.2023.12021261202126New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensorJanikua Nelson-Mora0Diana Rubio1Amairani Ventura-Martínez2Luis A. González3Diana Del-Rio4Diana Del-Rio5Yuli Aranda-López6Edgar Jiménez-Díaz7Edgar Jiménez-Díaz8Diego Zamarrón-Hernández9Diana G. Ríos-López10Stephanie Aguirre11Yasab Ruiz-Hernandez12Aarón Cruz-Ramírez13Jonás S. Barjau14Miguel A. Jáurez15Jehú Lopez-Aparicio16Andrea Campa-Higareda17Tatiana Fiordelisio18Tatiana Fiordelisio19Tatiana Fiordelisio20Unidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Neuroendocrinología Comparada-LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Neuroendocrinología Comparada-LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Imagenología Cuantitativa, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Biología Molecular y Diagnóstico, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Neuroendocrinología Comparada-LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoUnidad de Imagenología Cuantitativa, Laboratorio Nacional de Soluciones Biomiméticas para Diagnóstico y Terapia LaNSBioDyT, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoThe outbreak of COVID-19, a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, is regarded as the most severe of the documented coronavirus pandemics. The measurement and monitoring of SARS-CoV-2 antibody levels by serological tests are relevant for a better epidemiological and clinical understanding of COVID-19. The aim of this work was to design a method called the SARS-CoV-2 antibody detection method (SARS-CoV-2 AbDM) for fluorescence immunodetection of anti-SARS-CoV-2 IgG and IgM on both plate and microfluidic chip. For this purpose, a system with magnetic beads that immobilize the antigen (S protein and RBD) on its surface was used to determine the presence and quantity of antibodies in a sample in a single reaction. The SARS-CoV-2 AbDM led to several advantages in the performance of the tests, such as reduced cost, possibility of performing isolated or multiple samples, potential of multiplex detection, and capacity to detect whole blood samples without losing resolution. In addition, due to the microfluidic chip in conjunction with the motorized actuated platform, the time, sample quantity, and operator intervention during the process were reduced. All these advantages suggest that the SARS-CoV-2 AbDM has the potential to be developed as a PoC that can be used as a tool for seroprevalence monitoring, allowing a better understanding of the epidemiological and clinical characteristics of COVID-19 and contributing to more effective and ethical decision-making in strategies to fight against the COVID-19 pandemic.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202126/fullfluorescenceimmunodetectionmicrofluidic chipCOVID-19magnetic beads |
| spellingShingle | Janikua Nelson-Mora Diana Rubio Amairani Ventura-Martínez Luis A. González Diana Del-Rio Diana Del-Rio Yuli Aranda-López Edgar Jiménez-Díaz Edgar Jiménez-Díaz Diego Zamarrón-Hernández Diana G. Ríos-López Stephanie Aguirre Yasab Ruiz-Hernandez Aarón Cruz-Ramírez Jonás S. Barjau Miguel A. Jáurez Jehú Lopez-Aparicio Andrea Campa-Higareda Tatiana Fiordelisio Tatiana Fiordelisio Tatiana Fiordelisio New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor Frontiers in Bioengineering and Biotechnology fluorescence immunodetection microfluidic chip COVID-19 magnetic beads |
| title | New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor |
| title_full | New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor |
| title_fullStr | New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor |
| title_full_unstemmed | New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor |
| title_short | New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensor |
| title_sort | new detection method of sars cov 2 antibodies toward a point of care biosensor |
| topic | fluorescence immunodetection microfluidic chip COVID-19 magnetic beads |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202126/full |
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