Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
One of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound hea...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/10/518 |
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| author | Elham Alinezhad Sardareh Moloud Shahzeidi Mohammad Taha Salmanifard Ardestani Mohammad Mousavi-Khattat Atefeh Zarepour Ali Zarrabi |
| author_facet | Elham Alinezhad Sardareh Moloud Shahzeidi Mohammad Taha Salmanifard Ardestani Mohammad Mousavi-Khattat Atefeh Zarepour Ali Zarrabi |
| author_sort | Elham Alinezhad Sardareh |
| collection | DOAJ |
| description | One of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound healing research. In this study, gelatin and poly lactic acid (PLA) polymers were used in a wound dressing textile to provide gelation and structure strength properties, respectively. Meanwhile, silver nanoparticles (SNPs) synthesized through the green method were integrated into these fibers to provide the formed textile with antibacterial properties. Nanoparticles were made using donkey dung extract, and nanofibers were produced by the solution blow spinning method which has high production efficiency and low energy consumption among spinning methods. The produced nanoparticles were characterized and evaluated by UV-Vis, DLS, XRD, and FTIR methods, and the production of silver nanoparticles that were coated with metabolites in the extract was proven. In addition, the morphology and diameter of the resulted fibers and presence of nanoparticles were confirmed by the SEM method. The size and size distribution of the synthesized fibers were determined through analyzing SEM results. Gelatin nanofibers demonstrated a mean size of 743 nm before and 773 nm after nanoparticle coating. PLA nanofibers demonstrated a mean size of 57 nm before and 182 nm after nanoparticle coating. Finally, 335 nm was the mean diameter size of gelatin/PLA/SNPs nanofibers. Also, the textiles synthesized by PLA and gelatin which contained silver nanoparticles showed higher antibacterial activity against both gram-positive and gram-negative species compared to PLA and gelatin tissues without nanoparticles. Cytotoxicity test on L929 cells showed that silver nanoparticles incorporated textiles of PLA and gelatin show a very low level and non-significant toxicity compared to the free particles. |
| first_indexed | 2024-03-09T20:42:20Z |
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| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T20:42:20Z |
| publishDate | 2022-10-01 |
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| series | Bioengineering |
| spelling | doaj.art-f5e161f4b1754ffd8deeee5ebd03e6392023-11-23T22:57:14ZengMDPI AGBioengineering2306-53542022-10-0191051810.3390/bioengineering9100518Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing BacteriaElham Alinezhad Sardareh0Moloud Shahzeidi1Mohammad Taha Salmanifard Ardestani2Mohammad Mousavi-Khattat3Atefeh Zarepour4Ali Zarrabi5Department of Biology, Nourdanesh Institute of Higher Education, Meymeh, Isfahan 83517-65851, IranDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan 81593-58686, IranDepartment of Biology, Faculty of Science, Yazd University, Yazd 89158-18411, IranDepartment of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 81746-73441, IranDepartment of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul 34396, TurkeyDepartment of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul 34396, TurkeyOne of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound healing research. In this study, gelatin and poly lactic acid (PLA) polymers were used in a wound dressing textile to provide gelation and structure strength properties, respectively. Meanwhile, silver nanoparticles (SNPs) synthesized through the green method were integrated into these fibers to provide the formed textile with antibacterial properties. Nanoparticles were made using donkey dung extract, and nanofibers were produced by the solution blow spinning method which has high production efficiency and low energy consumption among spinning methods. The produced nanoparticles were characterized and evaluated by UV-Vis, DLS, XRD, and FTIR methods, and the production of silver nanoparticles that were coated with metabolites in the extract was proven. In addition, the morphology and diameter of the resulted fibers and presence of nanoparticles were confirmed by the SEM method. The size and size distribution of the synthesized fibers were determined through analyzing SEM results. Gelatin nanofibers demonstrated a mean size of 743 nm before and 773 nm after nanoparticle coating. PLA nanofibers demonstrated a mean size of 57 nm before and 182 nm after nanoparticle coating. Finally, 335 nm was the mean diameter size of gelatin/PLA/SNPs nanofibers. Also, the textiles synthesized by PLA and gelatin which contained silver nanoparticles showed higher antibacterial activity against both gram-positive and gram-negative species compared to PLA and gelatin tissues without nanoparticles. Cytotoxicity test on L929 cells showed that silver nanoparticles incorporated textiles of PLA and gelatin show a very low level and non-significant toxicity compared to the free particles.https://www.mdpi.com/2306-5354/9/10/518nanofiberssolution blow spinninghemolysisPLAgelatinantibacterial |
| spellingShingle | Elham Alinezhad Sardareh Moloud Shahzeidi Mohammad Taha Salmanifard Ardestani Mohammad Mousavi-Khattat Atefeh Zarepour Ali Zarrabi Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria Bioengineering nanofibers solution blow spinning hemolysis PLA gelatin antibacterial |
| title | Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria |
| title_full | Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria |
| title_fullStr | Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria |
| title_full_unstemmed | Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria |
| title_short | Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria |
| title_sort | antimicrobial activity of blow spun pla gelatin nanofibers containing green synthesized silver nanoparticles against wound infection causing bacteria |
| topic | nanofibers solution blow spinning hemolysis PLA gelatin antibacterial |
| url | https://www.mdpi.com/2306-5354/9/10/518 |
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