OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542
Aim. Detection of the peculiarities of oil oxidation and bio-surfactant production by soil strains of microorganisms of the genus Pseudomonas fluorescens ONU541, Bacillus megaterium ONU542. Methods. Species belonging to the investigated microorganisms were determined from the spectra of fatty acids...
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2017-06-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
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| Online Access: | http://mbt.onu.edu.ua/article/view/105021 |
| _version_ | 1818880825016451072 |
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| author | О. Г. Горшкова Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва І. П. Конуп |
| author_facet | О. Г. Горшкова Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва І. П. Конуп |
| author_sort | О. Г. Горшкова |
| collection | DOAJ |
| description | Aim. Detection of the peculiarities of oil oxidation and bio-surfactant production by soil strains of microorganisms of the genus Pseudomonas fluorescens ONU541, Bacillus megaterium ONU542. Methods. Species belonging to the investigated microorganisms were determined from the spectra of fatty acids of their cellular lipids. The residual content of petroleum hydrocarbons was determined by IRspectrometry on "IKS-29" in the range of 2700–3200 cm-1. The products of microorganisms of biosurfactants were determined by the decrease in the surface tension of liquid cultures of bacteria and their supernatants and by their emulsifying ability (by the emulsification index – E24,%). Results. The dependence between the oil destructive ability of the strains of bacteria studied and their ability to produce biosurfactants is shown. The P. fluorescens ONU541 strain is more than the B. megaterium strain ONU542 oxidizes oil (by 74.6%) and produces surfaceactive metabolites. The studied strains of P. fluorescens ONU541, B. megaterium ONU542 are able to produce biosurfactants provided they are cultivated for five days in a nutrient medium (NM) of composition (g/l): KH2PO4 – 1.5; Na2HPO4–3; NaCl–5; NH4Cl–1; glucose–2 (pH 7.0–7.2). Conclusion. Oil destructive activity and the ability to produce biosurfactants of soil strains of P. fluorescens ONU541, B. megaterium ONU542 have been determined. It has been proved that in the joint presence of the investigated strains of microorganisms, the degree of destruction of oil reaches 90% under harsh environmental conditions: during cleaning of saline soil sections of the Zmeiny Island with chronic oil contamination. |
| first_indexed | 2024-12-19T14:52:07Z |
| format | Article |
| id | doaj.art-f5e1c3adbd414a51bc59c5ff26211099 |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T14:52:07Z |
| publishDate | 2017-06-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-f5e1c3adbd414a51bc59c5ff262110992022-12-21T20:16:49ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632017-06-0102(38)617110.18524/2307-4663.2017.2(38).105021105021OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542О. Г. Горшкова0Т. В. Гудзенко1О. В. Волювач2Т. О. Бєляєва3І. П. Конуп4Одеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаОдеський національний університет імені І.І. МечниковаAim. Detection of the peculiarities of oil oxidation and bio-surfactant production by soil strains of microorganisms of the genus Pseudomonas fluorescens ONU541, Bacillus megaterium ONU542. Methods. Species belonging to the investigated microorganisms were determined from the spectra of fatty acids of their cellular lipids. The residual content of petroleum hydrocarbons was determined by IRspectrometry on "IKS-29" in the range of 2700–3200 cm-1. The products of microorganisms of biosurfactants were determined by the decrease in the surface tension of liquid cultures of bacteria and their supernatants and by their emulsifying ability (by the emulsification index – E24,%). Results. The dependence between the oil destructive ability of the strains of bacteria studied and their ability to produce biosurfactants is shown. The P. fluorescens ONU541 strain is more than the B. megaterium strain ONU542 oxidizes oil (by 74.6%) and produces surfaceactive metabolites. The studied strains of P. fluorescens ONU541, B. megaterium ONU542 are able to produce biosurfactants provided they are cultivated for five days in a nutrient medium (NM) of composition (g/l): KH2PO4 – 1.5; Na2HPO4–3; NaCl–5; NH4Cl–1; glucose–2 (pH 7.0–7.2). Conclusion. Oil destructive activity and the ability to produce biosurfactants of soil strains of P. fluorescens ONU541, B. megaterium ONU542 have been determined. It has been proved that in the joint presence of the investigated strains of microorganisms, the degree of destruction of oil reaches 90% under harsh environmental conditions: during cleaning of saline soil sections of the Zmeiny Island with chronic oil contamination.http://mbt.onu.edu.ua/article/view/105021pseudomonas fluorescens onu541bacillus megaterium onu542деструктори нафтипродуценти поверхнево-активних речовин |
| spellingShingle | О. Г. Горшкова Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва І. П. Конуп OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 Mìkrobìologìâ ì Bìotehnologìâ pseudomonas fluorescens onu541 bacillus megaterium onu542 деструктори нафти продуценти поверхнево-активних речовин |
| title | OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 |
| title_full | OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 |
| title_fullStr | OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 |
| title_full_unstemmed | OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 |
| title_short | OIL OXIDIZATION AND BIO-SAFACTANTS PRODUCTION BY STRAINS OF P. FLUORESCENS ONU541 AND B. MEGATERIUM ONU542 |
| title_sort | oil oxidization and bio safactants production by strains of p fluorescens onu541 and b megaterium onu542 |
| topic | pseudomonas fluorescens onu541 bacillus megaterium onu542 деструктори нафти продуценти поверхнево-активних речовин |
| url | http://mbt.onu.edu.ua/article/view/105021 |
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