Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model
Chronic lymphedema after cancer treatment is common and there is still no cure for this disease. We herein investigated the lymphangiogenic capacity of adipose tissue-derived microvascular fragments (MVF), which contain stem cells and lymphatic vessel fragments. Secondary lymphedema was induced in t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-07-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314221109957 |
| _version_ | 1818012966397673472 |
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| author | Florian S Frueh Laura Gassert Claudia Scheuer Andreas Müller Peter Fries Anne S Boewe Emmanuel Ampofo Claudia E Rübe Michael D Menger Matthias W Laschke |
| author_facet | Florian S Frueh Laura Gassert Claudia Scheuer Andreas Müller Peter Fries Anne S Boewe Emmanuel Ampofo Claudia E Rübe Michael D Menger Matthias W Laschke |
| author_sort | Florian S Frueh |
| collection | DOAJ |
| description | Chronic lymphedema after cancer treatment is common and there is still no cure for this disease. We herein investigated the lymphangiogenic capacity of adipose tissue-derived microvascular fragments (MVF), which contain stem cells and lymphatic vessel fragments. Secondary lymphedema was induced in the hindlimbs of C57BL/6J mice. Green fluorescence protein (GFP) + MVF were isolated from transgenic C57BL/6Tg (CAG-EGFP)1Osb/J mice, suspended in collagen hydrogel, and injected in the lymphadenectomy defect of wild-type animals. This crossover model allowed the detection of MVF-derived blood and lymphatic vessels after transplantation. The MVF group was compared with animals receiving collagen hydrogel only or a sham intervention. Lymphangiogenic effects were analyzed using volumetry, magnetic resonance (MR) lymphography, histology, and immunohistochemistry. MVF injection resulted in reduced hindlimb volumes when compared to non-treated controls. MR lymphography revealed lymphatic regeneration with reduced dermal backflow after MVF treatment. Finally, MVF transplantation promoted popliteal angiogenesis and lymphangiogenesis associated with a significantly increased microvessel and lymphatic vessel density. These findings indicate that MVF transplantation represents a promising approach to induce therapeutic lymphangiogenesis. |
| first_indexed | 2024-04-14T06:26:47Z |
| format | Article |
| id | doaj.art-f5e23f791ef747c9b6bbf2e3aef03319 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-14T06:26:47Z |
| publishDate | 2022-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-f5e23f791ef747c9b6bbf2e3aef033192022-12-22T02:07:46ZengSAGE PublishingJournal of Tissue Engineering2041-73142022-07-011310.1177/20417314221109957Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema modelFlorian S Frueh0Laura Gassert1Claudia Scheuer2Andreas Müller3Peter Fries4Anne S Boewe5Emmanuel Ampofo6Claudia E Rübe7Michael D Menger8Matthias W Laschke9Institute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyClinic of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg/Saar, GermanyClinic of Diagnostic and Interventional Radiology, Saarland University Medical Center, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyDepartment of Radiotherapy and Radiation Oncology, Saarland University Medical Centre, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyInstitute for Clinical and Experimental Surgery, Saarland University, Homburg/Saar, GermanyChronic lymphedema after cancer treatment is common and there is still no cure for this disease. We herein investigated the lymphangiogenic capacity of adipose tissue-derived microvascular fragments (MVF), which contain stem cells and lymphatic vessel fragments. Secondary lymphedema was induced in the hindlimbs of C57BL/6J mice. Green fluorescence protein (GFP) + MVF were isolated from transgenic C57BL/6Tg (CAG-EGFP)1Osb/J mice, suspended in collagen hydrogel, and injected in the lymphadenectomy defect of wild-type animals. This crossover model allowed the detection of MVF-derived blood and lymphatic vessels after transplantation. The MVF group was compared with animals receiving collagen hydrogel only or a sham intervention. Lymphangiogenic effects were analyzed using volumetry, magnetic resonance (MR) lymphography, histology, and immunohistochemistry. MVF injection resulted in reduced hindlimb volumes when compared to non-treated controls. MR lymphography revealed lymphatic regeneration with reduced dermal backflow after MVF treatment. Finally, MVF transplantation promoted popliteal angiogenesis and lymphangiogenesis associated with a significantly increased microvessel and lymphatic vessel density. These findings indicate that MVF transplantation represents a promising approach to induce therapeutic lymphangiogenesis.https://doi.org/10.1177/20417314221109957 |
| spellingShingle | Florian S Frueh Laura Gassert Claudia Scheuer Andreas Müller Peter Fries Anne S Boewe Emmanuel Ampofo Claudia E Rübe Michael D Menger Matthias W Laschke Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model Journal of Tissue Engineering |
| title | Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| title_full | Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| title_fullStr | Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| title_full_unstemmed | Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| title_short | Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| title_sort | adipose tissue derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model |
| url | https://doi.org/10.1177/20417314221109957 |
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