Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus
Inhibitory γ-aminobutyric acid (GABA)-ergic interneurons mediate inhibition in neuronal circuitry and support normal brain function. Consequently, dysregulation of inhibition is implicated in various brain disorders. Parvalbumin (PV) and somatostatin (SST) interneurons, the two major types of GABAer...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1146278/full |
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| author | Tzu-Hsuan Huang Yi-Sian Lin Yi-Sian Lin Chiao-Wan Hsiao Chiao-Wan Hsiao Liang-Yun Wang Musa Iyiola Ajibola Musa Iyiola Ajibola Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Yu-Ling Lin Yu-Jui Li Cho-Yi Chen Cho-Yi Chen Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Irene Han-Juo Cheng Irene Han-Juo Cheng Irene Han-Juo Cheng |
| author_facet | Tzu-Hsuan Huang Yi-Sian Lin Yi-Sian Lin Chiao-Wan Hsiao Chiao-Wan Hsiao Liang-Yun Wang Musa Iyiola Ajibola Musa Iyiola Ajibola Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Yu-Ling Lin Yu-Jui Li Cho-Yi Chen Cho-Yi Chen Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Irene Han-Juo Cheng Irene Han-Juo Cheng Irene Han-Juo Cheng |
| author_sort | Tzu-Hsuan Huang |
| collection | DOAJ |
| description | Inhibitory γ-aminobutyric acid (GABA)-ergic interneurons mediate inhibition in neuronal circuitry and support normal brain function. Consequently, dysregulation of inhibition is implicated in various brain disorders. Parvalbumin (PV) and somatostatin (SST) interneurons, the two major types of GABAergic inhibitory interneurons in the hippocampus, exhibit distinct morpho-physiological properties and coordinate information processing and memory formation. However, the molecular mechanisms underlying the specialized properties of PV and SST interneurons remain unclear. This study aimed to compare the transcriptomic differences between these two classes of interneurons in the hippocampus using the ribosome tagging approach. The results revealed distinct expressions of genes such as voltage-gated ion channels and GABAA receptor subunits between PV and SST interneurons. Gabrd and Gabra6 were identified as contributors to the contrasting tonic GABAergic inhibition observed in PV and SST interneurons. Moreover, some of the differentially expressed genes were associated with schizophrenia and epilepsy. In conclusion, our results provide molecular insights into the distinct roles of PV and SST interneurons in health and disease. |
| first_indexed | 2024-03-12T22:49:37Z |
| format | Article |
| id | doaj.art-f5e31084e68641afac963fc78b986bd5 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-03-12T22:49:37Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-f5e31084e68641afac963fc78b986bd52023-07-20T16:30:30ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-07-011710.3389/fncel.2023.11462781146278Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampusTzu-Hsuan Huang0Yi-Sian Lin1Yi-Sian Lin2Chiao-Wan Hsiao3Chiao-Wan Hsiao4Liang-Yun Wang5Musa Iyiola Ajibola6Musa Iyiola Ajibola7Wahab Imam Abdulmajeed8Wahab Imam Abdulmajeed9Wahab Imam Abdulmajeed10Yu-Ling Lin11Yu-Jui Li12Cho-Yi Chen13Cho-Yi Chen14Cheng-Chang Lien15Cheng-Chang Lien16Cheng-Chang Lien17Cheng-Chang Lien18Cheng-Di Chiu19Cheng-Di Chiu20Cheng-Di Chiu21Cheng-Di Chiu22Irene Han-Juo Cheng23Irene Han-Juo Cheng24Irene Han-Juo Cheng25Institute of Brain Science, National Yang Ming Chiao Tung University, Taipei, TaiwanInstitute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei, TaiwanProgram in Genetics and Genomics, Baylor College of Medicine, Houston, TX, United StatesInstitute of Brain Science, National Yang Ming Chiao Tung University, Taipei, TaiwanProgram in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanInstitute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei, TaiwanInstitute of Neuroscience, National Yang Ming Chiao Tung University, Taipei, TaiwanTaiwan International Graduate Program in Interdisciplinary Neuroscience, College of Life Sciences, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanInstitute of Neuroscience, National Yang Ming Chiao Tung University, Taipei, TaiwanTaiwan International Graduate Program in Interdisciplinary Neuroscience, College of Life Sciences, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanDepartment of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Ilorin, NigeriaInstitute of Neuroscience, National Yang Ming Chiao Tung University, Taipei, TaiwanInstitute of Neuroscience, National Yang Ming Chiao Tung University, Taipei, TaiwanInstitute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei, TaiwanBrain Research Center, National Yang Ming Chiao Tung University, Taipei, TaiwanProgram in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanInstitute of Neuroscience, National Yang Ming Chiao Tung University, Taipei, TaiwanTaiwan International Graduate Program in Interdisciplinary Neuroscience, College of Life Sciences, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanBrain Research Center, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartment of Neurosurgery, China Medical University Hospital, Taichung, Taiwan0Spine Center, China Medical University Hospital, Taichung, Taiwan1Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan2School of Medicine, China Medical University, Taichung, TaiwanInstitute of Brain Science, National Yang Ming Chiao Tung University, Taipei, TaiwanProgram in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, TaiwanBrain Research Center, National Yang Ming Chiao Tung University, Taipei, TaiwanInhibitory γ-aminobutyric acid (GABA)-ergic interneurons mediate inhibition in neuronal circuitry and support normal brain function. Consequently, dysregulation of inhibition is implicated in various brain disorders. Parvalbumin (PV) and somatostatin (SST) interneurons, the two major types of GABAergic inhibitory interneurons in the hippocampus, exhibit distinct morpho-physiological properties and coordinate information processing and memory formation. However, the molecular mechanisms underlying the specialized properties of PV and SST interneurons remain unclear. This study aimed to compare the transcriptomic differences between these two classes of interneurons in the hippocampus using the ribosome tagging approach. The results revealed distinct expressions of genes such as voltage-gated ion channels and GABAA receptor subunits between PV and SST interneurons. Gabrd and Gabra6 were identified as contributors to the contrasting tonic GABAergic inhibition observed in PV and SST interneurons. Moreover, some of the differentially expressed genes were associated with schizophrenia and epilepsy. In conclusion, our results provide molecular insights into the distinct roles of PV and SST interneurons in health and disease.https://www.frontiersin.org/articles/10.3389/fncel.2023.1146278/fulltonic inhibitionsomatostatin (SST)parvalbumin (PV)RiboTagGABAA receptorGABRD |
| spellingShingle | Tzu-Hsuan Huang Yi-Sian Lin Yi-Sian Lin Chiao-Wan Hsiao Chiao-Wan Hsiao Liang-Yun Wang Musa Iyiola Ajibola Musa Iyiola Ajibola Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Wahab Imam Abdulmajeed Yu-Ling Lin Yu-Jui Li Cho-Yi Chen Cho-Yi Chen Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Chang Lien Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Cheng-Di Chiu Irene Han-Juo Cheng Irene Han-Juo Cheng Irene Han-Juo Cheng Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus Frontiers in Cellular Neuroscience tonic inhibition somatostatin (SST) parvalbumin (PV) RiboTag GABAA receptor GABRD |
| title | Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| title_full | Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| title_fullStr | Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| title_full_unstemmed | Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| title_short | Differential expression of GABAA receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| title_sort | differential expression of gabaa receptor subunits δ and α6 mediates tonic inhibition in parvalbumin and somatostatin interneurons in the mouse hippocampus |
| topic | tonic inhibition somatostatin (SST) parvalbumin (PV) RiboTag GABAA receptor GABRD |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1146278/full |
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