Citral modulates human monocyte responses to Staphylococcus aureus infection
Abstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01536-4 |
| _version_ | 1818918892246925312 |
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| author | Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques |
| author_facet | Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques |
| author_sort | Hellen Braga Martins Oliveira |
| collection | DOAJ |
| description | Abstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the present study aimed to investigate the modulating role of citral in inflammation caused by S. aureus infection. For this, we used an isolate obtained from a nasal swab sample of a healthy child attending a day-care centre in Vitória da Conquista, Bahia, Brazil. The role of citral in modulating immunological factors against S. aureus infection was evaluated by isolating and cultivating human peripheral blood mononuclear cells. The monocytes were treated with 4%, 2%, and 1% citral before and after inoculation with S. aureus. The cells were analysed by immunophenotyping of monocyte cell surface molecules (CD54, CD282, CD80, HLA-DR, and CD86) and cytokine dosage (IL-1β, IL-6, IL-10, IL-12p70, IL-23, IFN-γ, TGF-β, and TNF-α), and evaluated for the expression of 84 genes related to innate and adaptive immune system responses. GraphPad Prism software and variables with P values < 0.05, were used for statistical analysis. Our data demonstrated citral’s action on the expression of surface markers involved in recognition, presentation, and migration, such as CD14, CD54, and CD80, in global negative regulation of inflammation with inhibitory effects on NF-κB, JNK/p38, and IFN pathways. Consequently, IL-1β, IL-6, IL-12p70, IL-23, IFN-γ, and TNF-α cytokine expression was reduced in groups treated with citral and groups treated with citral at 4%, 2%, and 1% and infected, and levels of anti-inflammatory cytokines such as IL-10 were increased. Furthermore, citral could be used as a supporting anti-inflammatory agent against infections caused by S. aureus. There are no data correlating citral, S. aureus, and the markers analysed here; thus, our study addresses this gap in the literature. |
| first_indexed | 2024-12-20T00:57:11Z |
| format | Article |
| id | doaj.art-f5e3d110964d44c484a655bfbafd9696 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T00:57:11Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f5e3d110964d44c484a655bfbafd96962022-12-21T19:59:05ZengNature PortfolioScientific Reports2045-23222021-11-0111111310.1038/s41598-021-01536-4Citral modulates human monocyte responses to Staphylococcus aureus infectionHellen Braga Martins Oliveira0Nathan das Neves Selis1Thamara Louisy Santos Brito2Beatriz Almeida Sampaio3Rafaela de Souza Bittencourt4Caline Novais Teixeira Oliveira5Manoel Neres Santos Júnior6Carolline Florentino Almeida7Palloma Porto Almeida8Guilherme Barreto Campos9Aline Teixeira Amorim10Jorge Timenetsky11Carla Cristina Romano12Ana Paula Trovatti Uetanabaro13Regiane Yatsuda14Lucas Miranda Marques15Universidade Estadual de Santa CruzUniversidade Estadual de Santa CruzInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaUniversidade Estadual de Santa CruzUniversidade Estadual de Santa CruzInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaDepartamento de Biologia Geral, Universidade Federal da ViçosaInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaInstituto de Ciências Biomédicas, Universidade de São PauloInstituto de Ciências Biomédicas, Universidade de São PauloUniversidade Estadual de Santa CruzUniversidade Estadual de Santa CruzInstituto Multidisciplinar em Saúde, Universidade Federal da BahiaUniversidade Estadual de Santa CruzAbstract Staphylococcus aureus is a Gram-positive bacterium that is considered an important human pathogen. Due to its virulence and ability to acquire mechanisms of resistance to antibiotics, the clinical severity of S. aureus infection is driven by inflammatory responses to the bacteria. Thus, the present study aimed to investigate the modulating role of citral in inflammation caused by S. aureus infection. For this, we used an isolate obtained from a nasal swab sample of a healthy child attending a day-care centre in Vitória da Conquista, Bahia, Brazil. The role of citral in modulating immunological factors against S. aureus infection was evaluated by isolating and cultivating human peripheral blood mononuclear cells. The monocytes were treated with 4%, 2%, and 1% citral before and after inoculation with S. aureus. The cells were analysed by immunophenotyping of monocyte cell surface molecules (CD54, CD282, CD80, HLA-DR, and CD86) and cytokine dosage (IL-1β, IL-6, IL-10, IL-12p70, IL-23, IFN-γ, TGF-β, and TNF-α), and evaluated for the expression of 84 genes related to innate and adaptive immune system responses. GraphPad Prism software and variables with P values < 0.05, were used for statistical analysis. Our data demonstrated citral’s action on the expression of surface markers involved in recognition, presentation, and migration, such as CD14, CD54, and CD80, in global negative regulation of inflammation with inhibitory effects on NF-κB, JNK/p38, and IFN pathways. Consequently, IL-1β, IL-6, IL-12p70, IL-23, IFN-γ, and TNF-α cytokine expression was reduced in groups treated with citral and groups treated with citral at 4%, 2%, and 1% and infected, and levels of anti-inflammatory cytokines such as IL-10 were increased. Furthermore, citral could be used as a supporting anti-inflammatory agent against infections caused by S. aureus. There are no data correlating citral, S. aureus, and the markers analysed here; thus, our study addresses this gap in the literature.https://doi.org/10.1038/s41598-021-01536-4 |
| spellingShingle | Hellen Braga Martins Oliveira Nathan das Neves Selis Thamara Louisy Santos Brito Beatriz Almeida Sampaio Rafaela de Souza Bittencourt Caline Novais Teixeira Oliveira Manoel Neres Santos Júnior Carolline Florentino Almeida Palloma Porto Almeida Guilherme Barreto Campos Aline Teixeira Amorim Jorge Timenetsky Carla Cristina Romano Ana Paula Trovatti Uetanabaro Regiane Yatsuda Lucas Miranda Marques Citral modulates human monocyte responses to Staphylococcus aureus infection Scientific Reports |
| title | Citral modulates human monocyte responses to Staphylococcus aureus infection |
| title_full | Citral modulates human monocyte responses to Staphylococcus aureus infection |
| title_fullStr | Citral modulates human monocyte responses to Staphylococcus aureus infection |
| title_full_unstemmed | Citral modulates human monocyte responses to Staphylococcus aureus infection |
| title_short | Citral modulates human monocyte responses to Staphylococcus aureus infection |
| title_sort | citral modulates human monocyte responses to staphylococcus aureus infection |
| url | https://doi.org/10.1038/s41598-021-01536-4 |
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