Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract
Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule R...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006754 |
| _version_ | 1819099593923624960 |
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| author | Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri |
| author_facet | Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri |
| author_sort | Rajendra Kumar Gurumurthy |
| collection | DOAJ |
| description | Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021). |
| first_indexed | 2024-12-22T00:49:21Z |
| format | Article |
| id | doaj.art-f5eabe965e014c81a882de798a74ac0d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:49:21Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f5eabe965e014c81a882de798a74ac0d2022-12-21T18:44:29ZengElsevierSTAR Protocols2666-16672021-12-0124100969Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tractRajendra Kumar Gurumurthy0Naveen Kumar1Cindrilla Chumduri2Department of Molecular Biology, Max Planck Institute for Infection Biology, 10117 Berlin, GermanyChair of Microbiology, University of Würzburg, 97074 Würzburg, GermanyChair of Microbiology, University of Würzburg, 97074 Würzburg, Germany; Corresponding authorSummary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development. Here, we describe an optimized protocol for single-molecule RNA in situ hybridization (smRNA-ISH), immunohistochemistry, and cell lineage analysis of the female reproductive tract organs using commercially available smRNA-ISH probes, antibodies, and inducible Cre-mice. The high-resolution multispectral fluorescence imaging is performed using wide-field epifluorescence or confocal microscopy combined with a slide scanner.For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721006754MicroscopyGene ExpressionIn Situ Hybridization |
| spellingShingle | Rajendra Kumar Gurumurthy Naveen Kumar Cindrilla Chumduri Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract STAR Protocols Microscopy Gene Expression In Situ Hybridization |
| title | Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract |
| title_full | Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract |
| title_fullStr | Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract |
| title_full_unstemmed | Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract |
| title_short | Spatial analysis of organ-wide RNA, protein expression, and lineage tracing in the female mouse reproductive tract |
| title_sort | spatial analysis of organ wide rna protein expression and lineage tracing in the female mouse reproductive tract |
| topic | Microscopy Gene Expression In Situ Hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006754 |
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