The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts
The anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH–GHR–IGF1 signali...
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MDPI AG
2023-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1207 |
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| author | Weilu Zhang Shudai Lin Zhenhai Jiao Lilong An Tingting Xie Jiang Wu Li Zhang |
| author_facet | Weilu Zhang Shudai Lin Zhenhai Jiao Lilong An Tingting Xie Jiang Wu Li Zhang |
| author_sort | Weilu Zhang |
| collection | DOAJ |
| description | The anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH–GHR–IGF1 signaling pathway. Therefore, the amount of GHR and the integrity of its structure will affect animal growth and development. In the previous study, we found that the mouse <i>GHR</i> gene can transcribe a circular transcript named circGHR. Our group cloned the full-length of the mouse circGHR and analyzed its spatiotemporal expression profile. In this study, we further predicted the open reading frame of circGHR with bioinformatics, subsequently constructed a Flag-tagged protein vector and preliminarily verified its coding potential with western blot. Additionally, we found that circGHR could inhibit the proliferation of NCTC469 cells and has a tendency to inhibit cell apoptosis, while for C2C12 cells, it showed a tendency to inhibit cell proliferation and promote its differentiation. Overall, these results suggested that the mouse circGHR had the potential to encode proteins and affect cell proliferation, differentiation and apoptosis. |
| first_indexed | 2024-03-11T02:25:56Z |
| format | Article |
| id | doaj.art-f5eb19e680a14e878588a181430fca2f |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:25:56Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-f5eb19e680a14e878588a181430fca2f2023-11-18T10:34:17ZengMDPI AGGenes2073-44252023-05-01146120710.3390/genes14061207The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and MyoblastsWeilu Zhang0Shudai Lin1Zhenhai Jiao2Lilong An3Tingting Xie4Jiang Wu5Li Zhang6College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaExperimental Teaching Center, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH–GHR–IGF1 signaling pathway. Therefore, the amount of GHR and the integrity of its structure will affect animal growth and development. In the previous study, we found that the mouse <i>GHR</i> gene can transcribe a circular transcript named circGHR. Our group cloned the full-length of the mouse circGHR and analyzed its spatiotemporal expression profile. In this study, we further predicted the open reading frame of circGHR with bioinformatics, subsequently constructed a Flag-tagged protein vector and preliminarily verified its coding potential with western blot. Additionally, we found that circGHR could inhibit the proliferation of NCTC469 cells and has a tendency to inhibit cell apoptosis, while for C2C12 cells, it showed a tendency to inhibit cell proliferation and promote its differentiation. Overall, these results suggested that the mouse circGHR had the potential to encode proteins and affect cell proliferation, differentiation and apoptosis.https://www.mdpi.com/2073-4425/14/6/1207mousecircGHRNCTC1469C2C12cell proliferation |
| spellingShingle | Weilu Zhang Shudai Lin Zhenhai Jiao Lilong An Tingting Xie Jiang Wu Li Zhang The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts Genes mouse circGHR NCTC1469 C2C12 cell proliferation |
| title | The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts |
| title_full | The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts |
| title_fullStr | The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts |
| title_full_unstemmed | The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts |
| title_short | The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts |
| title_sort | mouse circghr regulates proliferation differentiation and apoptosis of hepatocytes and myoblasts |
| topic | mouse circGHR NCTC1469 C2C12 cell proliferation |
| url | https://www.mdpi.com/2073-4425/14/6/1207 |
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