Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity
Through metabolic analysis, the present research seeks to reveal the defense mechanisms activated by a heavy metals-resistant plant, <i>Sesuvium portulacastrum</i> L. In this regard, shifting metabolisms in this plant were investigated in different heavy metals-contaminated experimental...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/676 |
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| author | Emad A. Alsherif Mohammad Yaghoubi Khanghahi Carmine Crecchio Shereen Magdy Korany Renato Lustosa Sobrinho Hamada AbdElgawad |
| author_facet | Emad A. Alsherif Mohammad Yaghoubi Khanghahi Carmine Crecchio Shereen Magdy Korany Renato Lustosa Sobrinho Hamada AbdElgawad |
| author_sort | Emad A. Alsherif |
| collection | DOAJ |
| description | Through metabolic analysis, the present research seeks to reveal the defense mechanisms activated by a heavy metals-resistant plant, <i>Sesuvium portulacastrum</i> L. In this regard, shifting metabolisms in this plant were investigated in different heavy metals-contaminated experimental sites, which were 50, 100, 500, 1000, and 5000 m away from a man-fabricated sewage dumping lake, with a wide range of pollutant concentrations. Heavy metals contaminations in contaminated soil and their impact on mineral composition and microbial population were also investigated. The significant findings to emerge from this research were the modifications of nitrogen and carbon metabolisms in plant tissues to cope with heavy metal toxicity. Increased plant amylase enzymes activity in contaminated soils increased starch degradation to soluble sugars as a mechanism to mitigate stress impact. Furthermore, increased activity of sucrose phosphate synthase in contaminated plants led to more accumulation of sucrose. Moreover, no change in the content of sucrose hydrolyzing enzymes (vacuolar invertase and cytosolic invertase) in the contaminated sites can suggest the translocation of sucrose from shoot to root under stress. Similarly, although this study demonstrated a high level of malate in plants exposed to stress, caution must be applied in suggesting a strong link between organic acids and the activation of defense mechanisms in plants, since other key organic acids were not affected by stress. Therefore, activation of other defense mechanisms, especially antioxidant defense molecules including alpha and beta tocopherols, showed a greater role in protecting plants from heavy metals stress. Moreover, the increment in the content of some amino acids (e.g., glycine, alanine, glutamate, arginine, and ornithine) in plants under metal toxicity can be attributed to a high level of stress tolerance. Moreover, strategies in the excitation of the synthesis of the unsaturated fatty acids (oleic and palmitoleic) were involved in enhancing stress tolerance, which was unexpectedly associated with an increase in the accumulation of palmitic and stearic (saturated fatty acids). Taken together, it can be concluded that these multiple mechanisms were involved in the response to stress which may be cooperative and complementary with each other in inducing resistance to the plants. |
| first_indexed | 2024-03-11T09:29:56Z |
| format | Article |
| id | doaj.art-f5ed27fe64a4463c823ac400126dc0c8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:56Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-f5ed27fe64a4463c823ac400126dc0c82023-11-16T17:45:51ZengMDPI AGPlants2223-77472023-02-0112367610.3390/plants12030676Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal ToxicityEmad A. Alsherif0Mohammad Yaghoubi Khanghahi1Carmine Crecchio2Shereen Magdy Korany3Renato Lustosa Sobrinho4Hamada AbdElgawad5Biology Department, College of Science and Arts at Khulis, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, ItalyDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Agronomy, Federal University of Technology—Paraná (UTFPR), Pato Branco 85503-390, PR, BrazilBotany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62521, EgyptThrough metabolic analysis, the present research seeks to reveal the defense mechanisms activated by a heavy metals-resistant plant, <i>Sesuvium portulacastrum</i> L. In this regard, shifting metabolisms in this plant were investigated in different heavy metals-contaminated experimental sites, which were 50, 100, 500, 1000, and 5000 m away from a man-fabricated sewage dumping lake, with a wide range of pollutant concentrations. Heavy metals contaminations in contaminated soil and their impact on mineral composition and microbial population were also investigated. The significant findings to emerge from this research were the modifications of nitrogen and carbon metabolisms in plant tissues to cope with heavy metal toxicity. Increased plant amylase enzymes activity in contaminated soils increased starch degradation to soluble sugars as a mechanism to mitigate stress impact. Furthermore, increased activity of sucrose phosphate synthase in contaminated plants led to more accumulation of sucrose. Moreover, no change in the content of sucrose hydrolyzing enzymes (vacuolar invertase and cytosolic invertase) in the contaminated sites can suggest the translocation of sucrose from shoot to root under stress. Similarly, although this study demonstrated a high level of malate in plants exposed to stress, caution must be applied in suggesting a strong link between organic acids and the activation of defense mechanisms in plants, since other key organic acids were not affected by stress. Therefore, activation of other defense mechanisms, especially antioxidant defense molecules including alpha and beta tocopherols, showed a greater role in protecting plants from heavy metals stress. Moreover, the increment in the content of some amino acids (e.g., glycine, alanine, glutamate, arginine, and ornithine) in plants under metal toxicity can be attributed to a high level of stress tolerance. Moreover, strategies in the excitation of the synthesis of the unsaturated fatty acids (oleic and palmitoleic) were involved in enhancing stress tolerance, which was unexpectedly associated with an increase in the accumulation of palmitic and stearic (saturated fatty acids). Taken together, it can be concluded that these multiple mechanisms were involved in the response to stress which may be cooperative and complementary with each other in inducing resistance to the plants.https://www.mdpi.com/2223-7747/12/3/676amino acidantioxidantfatty acidheavy metalmetabolite<i>Sesuvium portulacastrum</i> L. |
| spellingShingle | Emad A. Alsherif Mohammad Yaghoubi Khanghahi Carmine Crecchio Shereen Magdy Korany Renato Lustosa Sobrinho Hamada AbdElgawad Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity Plants amino acid antioxidant fatty acid heavy metal metabolite <i>Sesuvium portulacastrum</i> L. |
| title | Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity |
| title_full | Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity |
| title_fullStr | Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity |
| title_full_unstemmed | Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity |
| title_short | Understanding the Active Mechanisms of Plant (<i>Sesuvium portulacastrum</i> L.) against Heavy Metal Toxicity |
| title_sort | understanding the active mechanisms of plant i sesuvium portulacastrum i l against heavy metal toxicity |
| topic | amino acid antioxidant fatty acid heavy metal metabolite <i>Sesuvium portulacastrum</i> L. |
| url | https://www.mdpi.com/2223-7747/12/3/676 |
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