Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota
The potato is the fourth major food crop in the world. Its cultivation can encounter problems, resulting in poor growth and reduced yield. Plant microbiota has shown an ability to increase growth and resistance. However, in the development of effective microbiota manipulation strategies, it is essen...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1743 |
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| author | Lisa Cangioli Marco Mancini Ada Baldi Camilla Fagorzi Simone Orlandini Francesca Vaccaro Alessio Mengoni |
| author_facet | Lisa Cangioli Marco Mancini Ada Baldi Camilla Fagorzi Simone Orlandini Francesca Vaccaro Alessio Mengoni |
| author_sort | Lisa Cangioli |
| collection | DOAJ |
| description | The potato is the fourth major food crop in the world. Its cultivation can encounter problems, resulting in poor growth and reduced yield. Plant microbiota has shown an ability to increase growth and resistance. However, in the development of effective microbiota manipulation strategies, it is essential to know the effect of environmental variables on microbiota composition and function. Here, we aimed to identify the differential impact of the site of cultivation and plant growth stage on potato rhizosphere microbiota. We performed a 16S rRNA gene amplicon sequencing analysis of rhizospheric soil collected from potato plants grown at four sites in central Italy during two phenological stages. Rhizomicrobiota was mainly composed of members of phyla Acidobacteriota, Actinobacteriota, Chloroflexi, and Proteobacteria and was affected by both the site of cultivation and the plant stages. However, cultivation sites overcome the effect of plant phenological stages. The PiCRUST analysis suggested a high abundance of functions related to the biosynthesis of the siderophore enterobactin. The presence of site-specific taxa and functional profiling of the microbiota could be further exploited in long-term studies to evaluate the possibility of developing biomarkers for traceability of the products and to exploit plant growth-promoting abilities in the native potato microbiota. |
| first_indexed | 2024-03-09T23:07:24Z |
| format | Article |
| id | doaj.art-f5ed4c62a008485fa5ef91e08673e334 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:07:24Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f5ed4c62a008485fa5ef91e08673e3342023-11-23T17:52:30ZengMDPI AGMicroorganisms2076-26072022-08-01109174310.3390/microorganisms10091743Effect of Site and Phenological Status on the Potato Bacterial RhizomicrobiotaLisa Cangioli0Marco Mancini1Ada Baldi2Camilla Fagorzi3Simone Orlandini4Francesca Vaccaro5Alessio Mengoni6Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyDepartment of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Firenze, ItalyDepartment of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Firenze, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyDepartment of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Firenze, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino, ItalyThe potato is the fourth major food crop in the world. Its cultivation can encounter problems, resulting in poor growth and reduced yield. Plant microbiota has shown an ability to increase growth and resistance. However, in the development of effective microbiota manipulation strategies, it is essential to know the effect of environmental variables on microbiota composition and function. Here, we aimed to identify the differential impact of the site of cultivation and plant growth stage on potato rhizosphere microbiota. We performed a 16S rRNA gene amplicon sequencing analysis of rhizospheric soil collected from potato plants grown at four sites in central Italy during two phenological stages. Rhizomicrobiota was mainly composed of members of phyla Acidobacteriota, Actinobacteriota, Chloroflexi, and Proteobacteria and was affected by both the site of cultivation and the plant stages. However, cultivation sites overcome the effect of plant phenological stages. The PiCRUST analysis suggested a high abundance of functions related to the biosynthesis of the siderophore enterobactin. The presence of site-specific taxa and functional profiling of the microbiota could be further exploited in long-term studies to evaluate the possibility of developing biomarkers for traceability of the products and to exploit plant growth-promoting abilities in the native potato microbiota.https://www.mdpi.com/2076-2607/10/9/1743potatorhizospheremicrobiotaecosystem functions |
| spellingShingle | Lisa Cangioli Marco Mancini Ada Baldi Camilla Fagorzi Simone Orlandini Francesca Vaccaro Alessio Mengoni Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota Microorganisms potato rhizosphere microbiota ecosystem functions |
| title | Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota |
| title_full | Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota |
| title_fullStr | Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota |
| title_full_unstemmed | Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota |
| title_short | Effect of Site and Phenological Status on the Potato Bacterial Rhizomicrobiota |
| title_sort | effect of site and phenological status on the potato bacterial rhizomicrobiota |
| topic | potato rhizosphere microbiota ecosystem functions |
| url | https://www.mdpi.com/2076-2607/10/9/1743 |
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