A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells
The modification of chitosan (CS) has greatly expanded its application in the field of medicine. In this study, low-molecular-weight chitosan was modified with arginine (Arg) by a simple method. The identification by the Fourier transform infrared spectra (FTIR) showed that Arg was successfully cova...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1040 |
| _version_ | 1797441656193024000 |
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| author | Jixian Luo Jiangfeng Chen Yan Liu Yongji He Wenjuan Dong |
| author_facet | Jixian Luo Jiangfeng Chen Yan Liu Yongji He Wenjuan Dong |
| author_sort | Jixian Luo |
| collection | DOAJ |
| description | The modification of chitosan (CS) has greatly expanded its application in the field of medicine. In this study, low-molecular-weight chitosan was modified with arginine (Arg) by a simple method. The identification by the Fourier transform infrared spectra (FTIR) showed that Arg was successfully covalently attached to the CS. Interestingly, Arg-CS was identified as nanoparticles by atomic force microscopy (AFM) and transmission electron microscopy (TEM), whose particle size was 75.76 ± 12.07 nm based on Dynamic Light Scattering (DLS) characterization. Then, whether the prepared Arg-CS nanoparticles could encapsulate and deliver siRNA safely was investigated. Arg-CS was found to be able to encapsulate siRNAs in vitro via electrostatic interaction with siRNA; the Arg-CS/siRNA complex was safe for L1210 leukemia cells. Therefore, modification of chitosan by Arg produces novel nanoparticles to deliver siRNA into leukemia cells. This is the first time to identify Arg-CS as nanoparticles and explore their ability to deliver <i>Rhoa</i> siRNA into T-cell acute lymphoblastic leukemia (T-ALL) cells to advance therapies targeting Rhoa in the future. |
| first_indexed | 2024-03-09T12:27:18Z |
| format | Article |
| id | doaj.art-f5ef024121374d589d25f934b767fca9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:27:18Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f5ef024121374d589d25f934b767fca92023-11-30T22:34:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242104010.3390/ijms24021040A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia CellsJixian Luo0Jiangfeng Chen1Yan Liu2Yongji He3Wenjuan Dong4School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, ChinaInstitute of Functional Food of Shanxi, Shanxi Agricultural University, 79 Longcheng Street, Taiyuan 030031, ChinaInstitute of Environmental Science, Shanxi University, 92 Wucheng Road, Taiyuan 030006, ChinaThe modification of chitosan (CS) has greatly expanded its application in the field of medicine. In this study, low-molecular-weight chitosan was modified with arginine (Arg) by a simple method. The identification by the Fourier transform infrared spectra (FTIR) showed that Arg was successfully covalently attached to the CS. Interestingly, Arg-CS was identified as nanoparticles by atomic force microscopy (AFM) and transmission electron microscopy (TEM), whose particle size was 75.76 ± 12.07 nm based on Dynamic Light Scattering (DLS) characterization. Then, whether the prepared Arg-CS nanoparticles could encapsulate and deliver siRNA safely was investigated. Arg-CS was found to be able to encapsulate siRNAs in vitro via electrostatic interaction with siRNA; the Arg-CS/siRNA complex was safe for L1210 leukemia cells. Therefore, modification of chitosan by Arg produces novel nanoparticles to deliver siRNA into leukemia cells. This is the first time to identify Arg-CS as nanoparticles and explore their ability to deliver <i>Rhoa</i> siRNA into T-cell acute lymphoblastic leukemia (T-ALL) cells to advance therapies targeting Rhoa in the future.https://www.mdpi.com/1422-0067/24/2/1040chitosanargininemodificationsiRNAtransfection |
| spellingShingle | Jixian Luo Jiangfeng Chen Yan Liu Yongji He Wenjuan Dong A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells International Journal of Molecular Sciences chitosan arginine modification siRNA transfection |
| title | A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells |
| title_full | A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells |
| title_fullStr | A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells |
| title_full_unstemmed | A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells |
| title_short | A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells |
| title_sort | novel form of arginine chitosan as nanoparticles efficient for sirna delivery into mouse leukemia cells |
| topic | chitosan arginine modification siRNA transfection |
| url | https://www.mdpi.com/1422-0067/24/2/1040 |
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