The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients
Objectives: The results of the T-SPOT.TB (T-SPOT) assay are reduced in immunocompromised patients with active tuberculosis (ATB), and it is difficult using T-SPOT results to distinguish ATB from latent tuberculosis infection (LTBI) in this condition. The aim of this study was to determine the perfor...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971217301108 |
| _version_ | 1819212938805772288 |
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| author | Munyemana Jean Bosco Hongyan Hou Lie Mao Xiaohui Wu Kreeti Devi Ramroop Yanfang Lu Liyan Mao Yu Zhou Ziyong Sun Feng Wang |
| author_facet | Munyemana Jean Bosco Hongyan Hou Lie Mao Xiaohui Wu Kreeti Devi Ramroop Yanfang Lu Liyan Mao Yu Zhou Ziyong Sun Feng Wang |
| author_sort | Munyemana Jean Bosco |
| collection | DOAJ |
| description | Objectives: The results of the T-SPOT.TB (T-SPOT) assay are reduced in immunocompromised patients with active tuberculosis (ATB), and it is difficult using T-SPOT results to distinguish ATB from latent tuberculosis infection (LTBI) in this condition. The aim of this study was to determine the performance of the TBAg/PHA ratio in T-SPOT assay in the diagnosis of ATB in immunocompromised patients.
Methods: One hundred and forty three immunocompromised ATB patients and 124 LTBI individuals were diagnosed according to conventional tests and T-SPOT assay.
Results: The results of T-SPOT assay are of no value in the diagnosis of ATB in immunocompromised patients. However, the number of phytohaemagglutinin (PHA) spot-forming cells (sfc) in T-SPOT assay was substantially decreased in immunocompromised ATB patients compared with that in LTBI individuals. Receiver operating characteristic (ROC) analysis revealed that a further calculation of the TBAg/PHA ratio (the larger of the ESAT-6/PHA and CFP-10/PHA) showed a better performance in distinguishing these two diseases. Using the threshold value of 0.316, the sensitivity and specificity for distinguishing immunocompromised ATB patients from LTBI individuals were respectively 79.21 and 94.05%.
Conclusions: Our findings suggest that the TBAg/PHA ratio might have some significance for the diagnosis of TB disease in immunocompromised patients. |
| first_indexed | 2024-12-23T06:50:55Z |
| format | Article |
| id | doaj.art-f5f0a5db2529483a8104ef98af1654c9 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-23T06:50:55Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-f5f0a5db2529483a8104ef98af1654c92022-12-21T17:56:27ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112017-06-0159C556010.1016/j.ijid.2017.03.025The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patientsMunyemana Jean BoscoHongyan HouLie MaoXiaohui WuKreeti Devi RamroopYanfang LuLiyan MaoYu ZhouZiyong SunFeng WangObjectives: The results of the T-SPOT.TB (T-SPOT) assay are reduced in immunocompromised patients with active tuberculosis (ATB), and it is difficult using T-SPOT results to distinguish ATB from latent tuberculosis infection (LTBI) in this condition. The aim of this study was to determine the performance of the TBAg/PHA ratio in T-SPOT assay in the diagnosis of ATB in immunocompromised patients. Methods: One hundred and forty three immunocompromised ATB patients and 124 LTBI individuals were diagnosed according to conventional tests and T-SPOT assay. Results: The results of T-SPOT assay are of no value in the diagnosis of ATB in immunocompromised patients. However, the number of phytohaemagglutinin (PHA) spot-forming cells (sfc) in T-SPOT assay was substantially decreased in immunocompromised ATB patients compared with that in LTBI individuals. Receiver operating characteristic (ROC) analysis revealed that a further calculation of the TBAg/PHA ratio (the larger of the ESAT-6/PHA and CFP-10/PHA) showed a better performance in distinguishing these two diseases. Using the threshold value of 0.316, the sensitivity and specificity for distinguishing immunocompromised ATB patients from LTBI individuals were respectively 79.21 and 94.05%. Conclusions: Our findings suggest that the TBAg/PHA ratio might have some significance for the diagnosis of TB disease in immunocompromised patients.http://www.sciencedirect.com/science/article/pii/S1201971217301108T-SPOTactive TBimmunocompromised patientsTBAg/PHA ratio |
| spellingShingle | Munyemana Jean Bosco Hongyan Hou Lie Mao Xiaohui Wu Kreeti Devi Ramroop Yanfang Lu Liyan Mao Yu Zhou Ziyong Sun Feng Wang The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients International Journal of Infectious Diseases T-SPOT active TB immunocompromised patients TBAg/PHA ratio |
| title | The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients |
| title_full | The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients |
| title_fullStr | The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients |
| title_full_unstemmed | The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients |
| title_short | The performance of the TBAg/PHA ratio in the diagnosis of active TB disease in immunocompromised patients |
| title_sort | performance of the tbag pha ratio in the diagnosis of active tb disease in immunocompromised patients |
| topic | T-SPOT active TB immunocompromised patients TBAg/PHA ratio |
| url | http://www.sciencedirect.com/science/article/pii/S1201971217301108 |
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