Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet
As the link between plants and soils, rhizosphere soil microorganisms play an important role in the element cycle. This study aimed to understand the response of the rhizosphere soil microbial community structure and interaction network to grassland utilization in the alpine steppe of the northern T...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.931795/full |
| _version_ | 1811288703693225984 |
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| author | Lijiao Fu Lijiao Fu Yan Yan Xueqin Li Xueqin Li Yanling Liu Yanling Liu Xuyang Lu |
| author_facet | Lijiao Fu Lijiao Fu Yan Yan Xueqin Li Xueqin Li Yanling Liu Yanling Liu Xuyang Lu |
| author_sort | Lijiao Fu |
| collection | DOAJ |
| description | As the link between plants and soils, rhizosphere soil microorganisms play an important role in the element cycle. This study aimed to understand the response of the rhizosphere soil microbial community structure and interaction network to grassland utilization in the alpine steppe of the northern Tibet Plateau. High-throughput sequencing was employed to study the composition, diversity, and species interaction network of rhizosphere soil microbial communities under grazing, mowing, and enclosing treatments. Proteobacteria (47.19%) and Actinobacteria (42.20%) were the dominant bacteria in the rhizosphere soil. There was no significant difference in relative abundance among rhizosphere soil microorganisms at phylum and genus levels, but differences were found in Chlorobi, Ignavibacteriae, and Micromonospora. The alpha diversity index based on Shannon, Chao1, and Simpson indices revealed that except for a significant difference in the Shannon index of the Artemisia nanschanica group, the richness and evenness of rhizosphere soil microbial communities among all groups were similar. Non-metric multidimensional scaling (NMDS) and multi-response permutation procedure (MRPP) analyses showed that the inter-group differences of three plants (Stipa purpurea, Carex moorcroftii, and Artemisia nanschanica) were greater than the differences within the groups; however, only the inter-group difference with the Stipa purpurea group was significant. The microbial interaction network showed that the network complexity of the Artemisia nanschanica group and the enclosing treatment, which were not easily influenced by external factors, were higher than those of the other groups and treatments; this again demonstrated that Proteobacteria and Actinobacteria were the network core microbial species in alpine steppe of the northern Tibet Plateau and were crucial for maintaining stability of the microbial communities. Findings from this study provide a theoretical basis for the restoration of degraded alpine grassland and the development of microbial functions. |
| first_indexed | 2024-04-13T03:41:59Z |
| format | Article |
| id | doaj.art-f5f3075aeeea47adbca1a2c6d3881d57 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T03:41:59Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f5f3075aeeea47adbca1a2c6d3881d572022-12-22T03:04:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.931795931795Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern TibetLijiao Fu0Lijiao Fu1Yan Yan2Xueqin Li3Xueqin Li4Yanling Liu5Yanling Liu6Xuyang Lu7Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, ChinaChinese Academy of Sciences, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, ChinaChinese Academy of Sciences, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, ChinaChinese Academy of Sciences, University of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, ChinaAs the link between plants and soils, rhizosphere soil microorganisms play an important role in the element cycle. This study aimed to understand the response of the rhizosphere soil microbial community structure and interaction network to grassland utilization in the alpine steppe of the northern Tibet Plateau. High-throughput sequencing was employed to study the composition, diversity, and species interaction network of rhizosphere soil microbial communities under grazing, mowing, and enclosing treatments. Proteobacteria (47.19%) and Actinobacteria (42.20%) were the dominant bacteria in the rhizosphere soil. There was no significant difference in relative abundance among rhizosphere soil microorganisms at phylum and genus levels, but differences were found in Chlorobi, Ignavibacteriae, and Micromonospora. The alpha diversity index based on Shannon, Chao1, and Simpson indices revealed that except for a significant difference in the Shannon index of the Artemisia nanschanica group, the richness and evenness of rhizosphere soil microbial communities among all groups were similar. Non-metric multidimensional scaling (NMDS) and multi-response permutation procedure (MRPP) analyses showed that the inter-group differences of three plants (Stipa purpurea, Carex moorcroftii, and Artemisia nanschanica) were greater than the differences within the groups; however, only the inter-group difference with the Stipa purpurea group was significant. The microbial interaction network showed that the network complexity of the Artemisia nanschanica group and the enclosing treatment, which were not easily influenced by external factors, were higher than those of the other groups and treatments; this again demonstrated that Proteobacteria and Actinobacteria were the network core microbial species in alpine steppe of the northern Tibet Plateau and were crucial for maintaining stability of the microbial communities. Findings from this study provide a theoretical basis for the restoration of degraded alpine grassland and the development of microbial functions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.931795/fullrhizosphere soil microorganismscommunity structurediversityinteraction networkDNBSEQ sequencingalpine steppe |
| spellingShingle | Lijiao Fu Lijiao Fu Yan Yan Xueqin Li Xueqin Li Yanling Liu Yanling Liu Xuyang Lu Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet Frontiers in Microbiology rhizosphere soil microorganisms community structure diversity interaction network DNBSEQ sequencing alpine steppe |
| title | Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet |
| title_full | Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet |
| title_fullStr | Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet |
| title_full_unstemmed | Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet |
| title_short | Rhizosphere soil microbial community and its response to different utilization patterns in the semi-arid alpine grassland of northern Tibet |
| title_sort | rhizosphere soil microbial community and its response to different utilization patterns in the semi arid alpine grassland of northern tibet |
| topic | rhizosphere soil microorganisms community structure diversity interaction network DNBSEQ sequencing alpine steppe |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.931795/full |
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