Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR
Abstract Objective The objective of this study was to investigate the antibiotic susceptibility, virulence factors and clonal relationship among Pseudomonas aeruginosa isolated from environmental sources, hospitalized patients and the surfaces of cockroaches in the ICUs of four hospitals in Hamadan,...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-09-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3765-z |
| _version_ | 1818300774507085824 |
|---|---|
| author | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani |
| author_facet | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani |
| author_sort | Omid Zarei |
| collection | DOAJ |
| description | Abstract Objective The objective of this study was to investigate the antibiotic susceptibility, virulence factors and clonal relationship among Pseudomonas aeruginosa isolated from environmental sources, hospitalized patients and the surfaces of cockroaches in the ICUs of four hospitals in Hamadan, west of Iran. A total of 237, 286 and 156 bacterial isolates were collected from clinical, environmental and cockroach sources respectively from May to September, 2017. The antimicrobial susceptibility was determined using disk diffusion method. The virulence factors, exotoxins A, S and U were detected by PCR. The genetic linkage of P. aeruginosa isolates were analyzed by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. Results According to our findings, 58 (24.4%), 46 (16%) and 5 (3.25) P. aeruginosa were isolated from clinical, environmental and cockroach samples respectively. The MDR phenotypes were detected in 18 (45%) and 15 (37.5%) of clinical and environmental strains. The environmental isolates harbored more exoA and exoS than did clinical isolates. Genetic diversity was established among P. aeruginosa isolates as 14 different ERIC fingerprints were detected. The clonal relationships was detected among clinical, environmental and cockroach isolates. Our results highlighted the importance of identifying and controlling the potential sources of P. aeruginosa infections in hospitals. |
| first_indexed | 2024-12-13T05:12:28Z |
| format | Article |
| id | doaj.art-f5f3c0fb7c78404eb116267c36ed5851 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-13T05:12:28Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-f5f3c0fb7c78404eb116267c36ed58512022-12-21T23:58:30ZengBMCBMC Research Notes1756-05002018-09-011111710.1186/s13104-018-3765-zMolecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCROmid Zarei0Leili Shokoohizadeh1Hadi Hossainpour2Mohammad Yousef Alikhani3Student Research Committee, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesStudent Research Committee, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesAbstract Objective The objective of this study was to investigate the antibiotic susceptibility, virulence factors and clonal relationship among Pseudomonas aeruginosa isolated from environmental sources, hospitalized patients and the surfaces of cockroaches in the ICUs of four hospitals in Hamadan, west of Iran. A total of 237, 286 and 156 bacterial isolates were collected from clinical, environmental and cockroach sources respectively from May to September, 2017. The antimicrobial susceptibility was determined using disk diffusion method. The virulence factors, exotoxins A, S and U were detected by PCR. The genetic linkage of P. aeruginosa isolates were analyzed by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. Results According to our findings, 58 (24.4%), 46 (16%) and 5 (3.25) P. aeruginosa were isolated from clinical, environmental and cockroach samples respectively. The MDR phenotypes were detected in 18 (45%) and 15 (37.5%) of clinical and environmental strains. The environmental isolates harbored more exoA and exoS than did clinical isolates. Genetic diversity was established among P. aeruginosa isolates as 14 different ERIC fingerprints were detected. The clonal relationships was detected among clinical, environmental and cockroach isolates. Our results highlighted the importance of identifying and controlling the potential sources of P. aeruginosa infections in hospitals.http://link.springer.com/article/10.1186/s13104-018-3765-zPseudomonas aeruginosaERIC-PCRClinicalEnvironmentalCockroaches |
| spellingShingle | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR BMC Research Notes Pseudomonas aeruginosa ERIC-PCR Clinical Environmental Cockroaches |
| title | Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR |
| title_full | Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR |
| title_fullStr | Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR |
| title_full_unstemmed | Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR |
| title_short | Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR |
| title_sort | molecular analysis of pseudomonas aeruginosa isolated from clinical environmental and cockroach sources by eric pcr |
| topic | Pseudomonas aeruginosa ERIC-PCR Clinical Environmental Cockroaches |
| url | http://link.springer.com/article/10.1186/s13104-018-3765-z |
| work_keys_str_mv | AT omidzarei molecularanalysisofpseudomonasaeruginosaisolatedfromclinicalenvironmentalandcockroachsourcesbyericpcr AT leilishokoohizadeh molecularanalysisofpseudomonasaeruginosaisolatedfromclinicalenvironmentalandcockroachsourcesbyericpcr AT hadihossainpour molecularanalysisofpseudomonasaeruginosaisolatedfromclinicalenvironmentalandcockroachsourcesbyericpcr AT mohammadyousefalikhani molecularanalysisofpseudomonasaeruginosaisolatedfromclinicalenvironmentalandcockroachsourcesbyericpcr |