Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology
In this study, microbial cellulose production by Acetobacter xylinum 0416 using standardized liquid pineapple waste was carried out in a 4-L rotary disc reactor (RDR). The objective of this study was to optimize the process parameters for production of microbial cellulose in the RDR. The effects of...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-02-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_1858_Zahan_Fermentation_Rotary_Disc |
| _version_ | 1828416271989866496 |
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| author | Khairul Azly Zahan Norhayati Pa'e Ida Idayu Muhamad |
| author_facet | Khairul Azly Zahan Norhayati Pa'e Ida Idayu Muhamad |
| author_sort | Khairul Azly Zahan |
| collection | DOAJ |
| description | In this study, microbial cellulose production by Acetobacter xylinum 0416 using standardized liquid pineapple waste was carried out in a 4-L rotary disc reactor (RDR). The objective of this study was to optimize the process parameters for production of microbial cellulose in the RDR. The effects of the disc rotation speed (5 to 12 rpm), pH (3.5 to 7.5), fermentation period (3 to 6 days), and inoculum concentration (3 to 20% v/v) on the microbial cellulose production were investigated. The optimum microbial cellulose yield was obtained using 10% (v/v) of inoculum concentration, whereby four days’ duration gave the most productive yield. In addition, the highest production of microbial cellulose was obtained at a low disc rotation speed of 7 rpm and a pH of 5.0. Analysis of data performed a high coefficient of determination value (R2=0.875) represented by a mathematical model of optimized microbial cellulose production, Y = -200.437 + 7.180X1 + 69.869X2 + 4.369X3 + 1.867X4 – 0.512X12 – 6.766X22 – 0.585X32 – 0.079X42. From the results, it can be concluded that the foremost factors that affect the production of microbial cellulose in RDR were pH followed by inoculum concentration, disc rotation speed (rpm), and fermentation period. |
| first_indexed | 2024-12-10T14:01:13Z |
| format | Article |
| id | doaj.art-f5f64fe84fe444e282e3048bf730090b |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-10T14:01:13Z |
| publishDate | 2014-02-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-f5f64fe84fe444e282e3048bf730090b2022-12-22T01:45:48ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-02-01921858187210.15376/biores.9.2.1858-1872Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface MethodologyKhairul Azly Zahan0Norhayati Pa'e1Ida Idayu Muhamad2Universiti Teknologi MalaysiaUniversiti Teknologi MalaysiaUniversiti Teknologi MalaysiaIn this study, microbial cellulose production by Acetobacter xylinum 0416 using standardized liquid pineapple waste was carried out in a 4-L rotary disc reactor (RDR). The objective of this study was to optimize the process parameters for production of microbial cellulose in the RDR. The effects of the disc rotation speed (5 to 12 rpm), pH (3.5 to 7.5), fermentation period (3 to 6 days), and inoculum concentration (3 to 20% v/v) on the microbial cellulose production were investigated. The optimum microbial cellulose yield was obtained using 10% (v/v) of inoculum concentration, whereby four days’ duration gave the most productive yield. In addition, the highest production of microbial cellulose was obtained at a low disc rotation speed of 7 rpm and a pH of 5.0. Analysis of data performed a high coefficient of determination value (R2=0.875) represented by a mathematical model of optimized microbial cellulose production, Y = -200.437 + 7.180X1 + 69.869X2 + 4.369X3 + 1.867X4 – 0.512X12 – 6.766X22 – 0.585X32 – 0.079X42. From the results, it can be concluded that the foremost factors that affect the production of microbial cellulose in RDR were pH followed by inoculum concentration, disc rotation speed (rpm), and fermentation period.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_1858_Zahan_Fermentation_Rotary_DiscMicrobial celluloseRotary disc reactorProcess parametersOptimizationResponse surface methodologyPineapple wasteAcetobacter xylinum 0416 |
| spellingShingle | Khairul Azly Zahan Norhayati Pa'e Ida Idayu Muhamad Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology BioResources Microbial cellulose Rotary disc reactor Process parameters Optimization Response surface methodology Pineapple waste Acetobacter xylinum 0416 |
| title | Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology |
| title_full | Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology |
| title_fullStr | Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology |
| title_full_unstemmed | Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology |
| title_short | Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology |
| title_sort | process parameters for fermentation in a rotary disc reactor for optimum microbial cellulose production using response surface methodology |
| topic | Microbial cellulose Rotary disc reactor Process parameters Optimization Response surface methodology Pineapple waste Acetobacter xylinum 0416 |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_2_1858_Zahan_Fermentation_Rotary_Disc |
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