Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation
In the past three decades, a significant progress has been made in the prevention and treatment of gastric ulcers. The incidence of the disease has decreased, but gastric ulcer is still a medical problem. Currently, the available drugs for gastric ulcer treatment have many side effects; therefore, s...
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Elsevier
2023-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023028840 |
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| author | Maha B. Salem Mohamed Elzallat Dina Mostafa Mohammed Safia Samir Olfat A. Hammam Marwa Tamim A. Abdel-Wareth |
| author_facet | Maha B. Salem Mohamed Elzallat Dina Mostafa Mohammed Safia Samir Olfat A. Hammam Marwa Tamim A. Abdel-Wareth |
| author_sort | Maha B. Salem |
| collection | DOAJ |
| description | In the past three decades, a significant progress has been made in the prevention and treatment of gastric ulcers. The incidence of the disease has decreased, but gastric ulcer is still a medical problem. Currently, the available drugs for gastric ulcer treatment have many side effects; therefore, searching for new and safe therapeutic agents is mandatory. The present study aims to investigate the gastroprotective potential of Cornu aspersum (C. aspersum) mucin against gastric ulcers, and the mechanisms related to oxidative stress and inflammation. C. aspersum mucin was collected from 50 snails. The characteristics of C. aspersum mucin (chemical and microbiological) were evaluated. Mice were pretreated with famotidine and C. aspersum mucin (7.5 and 15 ml/kg b.w.) for 5 days, and then gastric ulcers were induced by indomethacin. Macroscopic examination, biochemical estimations, and Quantitative real-time PCR were carried out. Also, histopathological and immunohistopathological examinations were evaluated. We found that the high dose of the mucin significantly decreased the gastric mucosal malondialdehyde (MDA) and nitric oxide (NO) contents as well as interleukin 1β (IL-1β) and nuclear factor kappa β (NF-ҡB) expression, and inducible nitric oxide synthase (iNOS) immunostaining. It also increased the gastric mucosal GSH and catalase contents as well as hemoxygenase-1 (HO-1) and nuclear factor-erythroid 2-related factor 2 (Nrf2) expressions with regressions in gastric mucosal lesions. In conclusion, C. aspersum mucin could be a potential therapeutic candidate to protect against gastric ulceration. |
| first_indexed | 2024-03-13T08:26:23Z |
| format | Article |
| id | doaj.art-f5f906b4012848ff8fc6cac0ee661a8e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:26:23Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f5f906b4012848ff8fc6cac0ee661a8e2023-05-31T04:45:08ZengElsevierHeliyon2405-84402023-05-0195e15677Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammationMaha B. Salem0Mohamed Elzallat1Dina Mostafa Mohammed2Safia Samir3Olfat A. Hammam4Marwa Tamim A. Abdel-Wareth5Pharmacology Department, Theodor Bilharz Research Institute, Giza, EgyptImmunology Department, Theodor Bilharz Research Institute, Giza, EgyptNutrition and Food Sciences Department, National Research Centre, Giza, Egypt; Corresponding author.Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza, EgyptPathology Department, Theodor Bilharz Research Institute, Giza, EgyptEnvironmental Research Department, Theodor Bilharz Research Institute, Giza, EgyptIn the past three decades, a significant progress has been made in the prevention and treatment of gastric ulcers. The incidence of the disease has decreased, but gastric ulcer is still a medical problem. Currently, the available drugs for gastric ulcer treatment have many side effects; therefore, searching for new and safe therapeutic agents is mandatory. The present study aims to investigate the gastroprotective potential of Cornu aspersum (C. aspersum) mucin against gastric ulcers, and the mechanisms related to oxidative stress and inflammation. C. aspersum mucin was collected from 50 snails. The characteristics of C. aspersum mucin (chemical and microbiological) were evaluated. Mice were pretreated with famotidine and C. aspersum mucin (7.5 and 15 ml/kg b.w.) for 5 days, and then gastric ulcers were induced by indomethacin. Macroscopic examination, biochemical estimations, and Quantitative real-time PCR were carried out. Also, histopathological and immunohistopathological examinations were evaluated. We found that the high dose of the mucin significantly decreased the gastric mucosal malondialdehyde (MDA) and nitric oxide (NO) contents as well as interleukin 1β (IL-1β) and nuclear factor kappa β (NF-ҡB) expression, and inducible nitric oxide synthase (iNOS) immunostaining. It also increased the gastric mucosal GSH and catalase contents as well as hemoxygenase-1 (HO-1) and nuclear factor-erythroid 2-related factor 2 (Nrf2) expressions with regressions in gastric mucosal lesions. In conclusion, C. aspersum mucin could be a potential therapeutic candidate to protect against gastric ulceration.http://www.sciencedirect.com/science/article/pii/S2405844023028840Gastric ulcerIndomethacinCornu aspersumMucinOxidative stressInflammatory markers |
| spellingShingle | Maha B. Salem Mohamed Elzallat Dina Mostafa Mohammed Safia Samir Olfat A. Hammam Marwa Tamim A. Abdel-Wareth Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation Heliyon Gastric ulcer Indomethacin Cornu aspersum Mucin Oxidative stress Inflammatory markers |
| title | Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| title_full | Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| title_fullStr | Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| title_full_unstemmed | Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| title_short | Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| title_sort | cornu aspersum mucin attenuates indomethacins induced gastric ulcers in mice via alleviating oxidative stress and inflammation |
| topic | Gastric ulcer Indomethacin Cornu aspersum Mucin Oxidative stress Inflammatory markers |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023028840 |
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