Viroids as Companions of a Professional Career
Since the early 1970s when “virus-like” agents were considered as the cause of two diseases (potato spindle tuber and citrus exocortis), their study and further characterization have been linked to the development and use of molecular biology tools. Sucrose density gradient centr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/11/3/245 |
| _version_ | 1819081581194641408 |
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| author | Núria Duran-Vila |
| author_facet | Núria Duran-Vila |
| author_sort | Núria Duran-Vila |
| collection | DOAJ |
| description | Since the early 1970s when “virus-like” agents were considered as the cause of two diseases (potato spindle tuber and citrus exocortis), their study and further characterization have been linked to the development and use of molecular biology tools. Sucrose density gradient centrifugation and polyacrylamide gel electrophoresis (PAGE) played a critical role in the pioneering studies of PSTVd and citrus exocortis viroid (CEVd). This was later modified by using other PAGEs (sequential PAGE, return PAGE, two-dimensional PAGE), and/or different staining methods (ethidium bromide, silver nitrate, etc.). Since then, disease-causing agents suspected to be viroids were usually subjected to a number of tests to define their: (i) Molecular nature (RNA or DNA; single stranded or double stranded; circular or linear RNA); (ii) molecular weight; (iii) secondary and tertiary structure. Further biological assays are also essential to establish the relationship of a viroid with plant disease and to fulfill Koch’s postulates. |
| first_indexed | 2024-12-21T20:03:03Z |
| format | Article |
| id | doaj.art-f5fa2ede698a406f8d177ee6954a1f93 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T20:03:03Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f5fa2ede698a406f8d177ee6954a1f932022-12-21T18:51:55ZengMDPI AGViruses1999-49152019-03-0111324510.3390/v11030245v11030245Viroids as Companions of a Professional CareerNúria Duran-Vila0Centro de Protección Vegetal y Biotecnología. Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113 Valencia, SpainSince the early 1970s when “virus-like” agents were considered as the cause of two diseases (potato spindle tuber and citrus exocortis), their study and further characterization have been linked to the development and use of molecular biology tools. Sucrose density gradient centrifugation and polyacrylamide gel electrophoresis (PAGE) played a critical role in the pioneering studies of PSTVd and citrus exocortis viroid (CEVd). This was later modified by using other PAGEs (sequential PAGE, return PAGE, two-dimensional PAGE), and/or different staining methods (ethidium bromide, silver nitrate, etc.). Since then, disease-causing agents suspected to be viroids were usually subjected to a number of tests to define their: (i) Molecular nature (RNA or DNA; single stranded or double stranded; circular or linear RNA); (ii) molecular weight; (iii) secondary and tertiary structure. Further biological assays are also essential to establish the relationship of a viroid with plant disease and to fulfill Koch’s postulates.http://www.mdpi.com/1999-4915/11/3/245Viroid diseasesSymptom expressionCooperation approaches |
| spellingShingle | Núria Duran-Vila Viroids as Companions of a Professional Career Viruses Viroid diseases Symptom expression Cooperation approaches |
| title | Viroids as Companions of a Professional Career |
| title_full | Viroids as Companions of a Professional Career |
| title_fullStr | Viroids as Companions of a Professional Career |
| title_full_unstemmed | Viroids as Companions of a Professional Career |
| title_short | Viroids as Companions of a Professional Career |
| title_sort | viroids as companions of a professional career |
| topic | Viroid diseases Symptom expression Cooperation approaches |
| url | http://www.mdpi.com/1999-4915/11/3/245 |
| work_keys_str_mv | AT nuriaduranvila viroidsascompanionsofaprofessionalcareer |