| Summary: | Sucrose non-fermenting 1-related protein kinases (<i>SnRKs</i>) are a kind of plant-specific serine/threonine (Ser/Thr) protein kinase, which play an important role in plant stress resistance. However, the scale analysis of <i>SnRK</i> in the cucumber genome is currently unclear. In the study, a total of 30 <i>CsSnRK</i> genes were identified from genomic data. They were distributed on six chromosomes, including 1 <i>CsSnRK1</i>, 10 <i>CsSnRK2</i><i>s</i> and 19 <i>CsSnRK3</i><i>s</i>. According to the analysis of gene structure and motif composition, <i>CsSnRKs</i> showed obvious differences among the three subfamilies. The ratio of synonymous (Ks) and nonsynonymous (Ka) nucleotide substitutions (Ka/Ks) of three paralogues indicates that the <i>CsSnRK</i> gene family undergoes a purifying selection. The analysis of cis-acting elements shows that the promoter region of each <i>CsSnRK</i> gene contained different classes of hormone and stress-related cis-acting elements. Furthermore, based on RNA-sequencing data from the Short Read Archive (SRA) database of NCBI, the expression patterns of <i>CsSnRK</i> genes in six tissues were investigated, indicating that the expression of multiple <i>CsSnRK</i> genes was prevalent in these tissues. Transcription levels of <i>CsSnRK</i> genes after drought, methyl jasmonate (MeJA) and abscisic acid (ABA) treatments were analyzed by quantitative RT-PCR, and the results show that most of the <i>CsSnRK</i> genes responded to these stresses. However, under different treatments, individual genes played a major role. For example, under ABA treatment, <i>CsSnRK2.2</i> and <i>CsSnRK2.3</i> played a major role in the response to ABA. These results provide clear evidence that <i>CsSnRKs</i> may be involved in cucumber growth, development and stress response, and provide valuable information for future functional studies of <i>CsSnRKs</i>.
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