Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats
A microbial optosensor for nitrite was constructed based on biomimetic silica nanoparticles, which were doped with R5, a polypeptide component of silaffin, as a robust biosilica immobilization matrix entrapped with <i>Raoultella planticola</i> and NAD(P)H cofactor during the in vitro bio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/6/388 |
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| author | Siti Nur Syazni Mohd Zuki Choo Ta Goh Mohammad B. Kassim Ling Ling Tan |
| author_facet | Siti Nur Syazni Mohd Zuki Choo Ta Goh Mohammad B. Kassim Ling Ling Tan |
| author_sort | Siti Nur Syazni Mohd Zuki |
| collection | DOAJ |
| description | A microbial optosensor for nitrite was constructed based on biomimetic silica nanoparticles, which were doped with R5, a polypeptide component of silaffin, as a robust biosilica immobilization matrix entrapped with <i>Raoultella planticola</i> and NAD(P)H cofactor during the in vitro biosilicification process of silica nanoparticles. Ruthenium(II)(bipy)2(phenanthroline-benzoylthiourea), the chromophoric pH probe, was physically adsorbed on the resulting biogenic nanosilica. Optical quantitation of the nitrite concentration was performed via reflectance transduction of the bio-doped microbial nanosilica at a maximum reflectance of 608 nm, due to the deprotonation of phen-BT ligands in the ruthenium complex, while the intracellular enzyme expression system catalyzed the enzymatic reduction of nitrite. Reflectance enhancement of the microbial optosensor was linearly proportional to the nitrite concentration from 1–100 mg L<sup>−1</sup>, with a 0.25 mg L<sup>−1</sup> limit of detection and a rapid response time of 4 min. The proposed microbial optosensor showed good stability of >2 weeks, great repeatability for 5 repetitive assays (relative standard deviation, (RSD) = 0.2–1.4%), high reproducibility (RSD = 2.5%), and a negligible response to common interferents found in processed meats, such as NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup> ions, was observed. The microbial biosensor demonstrated an excellent capacity to provide an accurate estimation of nitrite in several cured meat samples via validation using a standard UV-vis spectrophotometric Griess assay. |
| first_indexed | 2024-03-10T00:18:26Z |
| format | Article |
| id | doaj.art-f6009087e79e4ff1bb435e6f7c513df1 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:18:26Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f6009087e79e4ff1bb435e6f7c513df12023-11-23T15:48:47ZengMDPI AGBiosensors2079-63742022-06-0112638810.3390/bios12060388Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured MeatsSiti Nur Syazni Mohd Zuki0Choo Ta Goh1Mohammad B. Kassim2Ling Ling Tan3Southeast Asia Disaster Prevention Research Initiative (SEADPRI), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor Darul Ehsan, MalaysiaSoutheast Asia Disaster Prevention Research Initiative (SEADPRI), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor Darul Ehsan, MalaysiaDepartment of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor Darul Ehsan, MalaysiaSoutheast Asia Disaster Prevention Research Initiative (SEADPRI), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, Bangi 43600, Selangor Darul Ehsan, MalaysiaA microbial optosensor for nitrite was constructed based on biomimetic silica nanoparticles, which were doped with R5, a polypeptide component of silaffin, as a robust biosilica immobilization matrix entrapped with <i>Raoultella planticola</i> and NAD(P)H cofactor during the in vitro biosilicification process of silica nanoparticles. Ruthenium(II)(bipy)2(phenanthroline-benzoylthiourea), the chromophoric pH probe, was physically adsorbed on the resulting biogenic nanosilica. Optical quantitation of the nitrite concentration was performed via reflectance transduction of the bio-doped microbial nanosilica at a maximum reflectance of 608 nm, due to the deprotonation of phen-BT ligands in the ruthenium complex, while the intracellular enzyme expression system catalyzed the enzymatic reduction of nitrite. Reflectance enhancement of the microbial optosensor was linearly proportional to the nitrite concentration from 1–100 mg L<sup>−1</sup>, with a 0.25 mg L<sup>−1</sup> limit of detection and a rapid response time of 4 min. The proposed microbial optosensor showed good stability of >2 weeks, great repeatability for 5 repetitive assays (relative standard deviation, (RSD) = 0.2–1.4%), high reproducibility (RSD = 2.5%), and a negligible response to common interferents found in processed meats, such as NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup> ions, was observed. The microbial biosensor demonstrated an excellent capacity to provide an accurate estimation of nitrite in several cured meat samples via validation using a standard UV-vis spectrophotometric Griess assay.https://www.mdpi.com/2079-6374/12/6/388biosensornitritesilaffinsilicaoptic |
| spellingShingle | Siti Nur Syazni Mohd Zuki Choo Ta Goh Mohammad B. Kassim Ling Ling Tan Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats Biosensors biosensor nitrite silaffin silica optic |
| title | Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats |
| title_full | Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats |
| title_fullStr | Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats |
| title_full_unstemmed | Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats |
| title_short | Bio-Doped Microbial Nanosilica as Optosensing Biomaterial for Visual Quantitation of Nitrite in Cured Meats |
| title_sort | bio doped microbial nanosilica as optosensing biomaterial for visual quantitation of nitrite in cured meats |
| topic | biosensor nitrite silaffin silica optic |
| url | https://www.mdpi.com/2079-6374/12/6/388 |
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