Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica
Depletion of hydrocarbons is forcing to find alternative resources to meet the energy demand of the growing population. Microbial biodiesel as a fuel can act as a cheaper and eco-friendly alternative to fossil fuel. Single-cell oil (SCO) consisting of carbon, hydrogen and oxygen grown over the ligno...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Energy Conversion and Management: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590174521000921 |
| _version_ | 1819261416987688960 |
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| author | Madhu Vasaki Murugan Sithan Gobinath Ravindran Balasubramanian Paramasivan Gayathiri Ekambaram Rama Rao Karri |
| author_facet | Madhu Vasaki Murugan Sithan Gobinath Ravindran Balasubramanian Paramasivan Gayathiri Ekambaram Rama Rao Karri |
| author_sort | Madhu Vasaki |
| collection | DOAJ |
| description | Depletion of hydrocarbons is forcing to find alternative resources to meet the energy demand of the growing population. Microbial biodiesel as a fuel can act as a cheaper and eco-friendly alternative to fossil fuel. Single-cell oil (SCO) consisting of carbon, hydrogen and oxygen grown over the lignocellulosic biomass using oleaginous microorganisms are triacylglycerols which can be converted to biodiesel, with physicochemical properties similar to conventional diesel. However, several cost-effective pretreatment methods are required to utilize lignocellulosic biomasses. The current research study investigates the SCO yield (and biodiesel characteristics) obtained from sugarcane bagasse hydrolysate through various pretreatment techniques. The pretreatment with 4% v/v H2SO4 at 25 min of ultra-sonication provided the best depolymerisation results (based on the glucose concentration). Yarrowia lipolytica was inoculated into the hydrolysates, allowed to grow at 25 °C, pH of 6.5 and rapid mixing for six days yielded biomass of 16.39 g/l. Biodiesel was extracted from the biomass via in-situ and ex-situ transesterification. In-situ transesterification carried out with the catalyst K2CO3 yields 80% biodiesel. In comparison, 63% were achieved with ex-situ transesterification, where lipid extraction was carried out as a first step and transesterified further in the presence of catalyst KOH to obtain biodiesel. The obtained fatty acid methyl esters (FAME) was subjected to FTIR analysis, and the observed physicochemical properties were within the international standards. |
| first_indexed | 2024-12-23T19:41:28Z |
| format | Article |
| id | doaj.art-f600b7df50b248aeb3dfc706eb5d6e72 |
| institution | Directory Open Access Journal |
| issn | 2590-1745 |
| language | English |
| last_indexed | 2024-12-23T19:41:28Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Energy Conversion and Management: X |
| spelling | doaj.art-f600b7df50b248aeb3dfc706eb5d6e722022-12-21T17:33:40ZengElsevierEnergy Conversion and Management: X2590-17452022-01-0113100167Biodiesel production from lignocellulosic biomass using Yarrowia lipolyticaMadhu Vasaki0Murugan Sithan1Gobinath Ravindran2Balasubramanian Paramasivan3Gayathiri Ekambaram4Rama Rao Karri5Department of Environmental Engineering, Government College of Technology Coimbatore, IndiaDepartment of Environmental Engineering, Government College of Technology Coimbatore, IndiaDepartment of Civil Engineering, S R Engineering College Warangal, Telangana, IndiaDepartment of Biotechnology & Medical Engineering, National Institute of Technology Rourkela, IndiaGuru Nanak College, Chennai, Tamilnadu, IndiaPetroleum and Chemical Engineering, Faculty of Engineering, Universiti Teknologi Brunei, Brunei Darussalam; Corresponding author.Depletion of hydrocarbons is forcing to find alternative resources to meet the energy demand of the growing population. Microbial biodiesel as a fuel can act as a cheaper and eco-friendly alternative to fossil fuel. Single-cell oil (SCO) consisting of carbon, hydrogen and oxygen grown over the lignocellulosic biomass using oleaginous microorganisms are triacylglycerols which can be converted to biodiesel, with physicochemical properties similar to conventional diesel. However, several cost-effective pretreatment methods are required to utilize lignocellulosic biomasses. The current research study investigates the SCO yield (and biodiesel characteristics) obtained from sugarcane bagasse hydrolysate through various pretreatment techniques. The pretreatment with 4% v/v H2SO4 at 25 min of ultra-sonication provided the best depolymerisation results (based on the glucose concentration). Yarrowia lipolytica was inoculated into the hydrolysates, allowed to grow at 25 °C, pH of 6.5 and rapid mixing for six days yielded biomass of 16.39 g/l. Biodiesel was extracted from the biomass via in-situ and ex-situ transesterification. In-situ transesterification carried out with the catalyst K2CO3 yields 80% biodiesel. In comparison, 63% were achieved with ex-situ transesterification, where lipid extraction was carried out as a first step and transesterified further in the presence of catalyst KOH to obtain biodiesel. The obtained fatty acid methyl esters (FAME) was subjected to FTIR analysis, and the observed physicochemical properties were within the international standards.http://www.sciencedirect.com/science/article/pii/S2590174521000921Single-cell oilLignocellulosic biomass hydrolysatesOleaginous microorganismYarrowia lipolyticaIn-situ transesterificationBiodiesel |
| spellingShingle | Madhu Vasaki Murugan Sithan Gobinath Ravindran Balasubramanian Paramasivan Gayathiri Ekambaram Rama Rao Karri Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica Energy Conversion and Management: X Single-cell oil Lignocellulosic biomass hydrolysates Oleaginous microorganism Yarrowia lipolytica In-situ transesterification Biodiesel |
| title | Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica |
| title_full | Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica |
| title_fullStr | Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica |
| title_full_unstemmed | Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica |
| title_short | Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica |
| title_sort | biodiesel production from lignocellulosic biomass using yarrowia lipolytica |
| topic | Single-cell oil Lignocellulosic biomass hydrolysates Oleaginous microorganism Yarrowia lipolytica In-situ transesterification Biodiesel |
| url | http://www.sciencedirect.com/science/article/pii/S2590174521000921 |
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