Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2019-04-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401&lng=en&tlng=en |
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| author | Vanessa Ghiggi Sorgatto Julio Cesar de Carvalho Eduardo Bittencourt Sydney Adriane Bianchi Pedroni Medeiros Luciana Porto de Souza Vandenberghe Carlos Ricardo Soccol |
| author_facet | Vanessa Ghiggi Sorgatto Julio Cesar de Carvalho Eduardo Bittencourt Sydney Adriane Bianchi Pedroni Medeiros Luciana Porto de Souza Vandenberghe Carlos Ricardo Soccol |
| author_sort | Vanessa Ghiggi Sorgatto |
| collection | DOAJ |
| description | Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes. |
| first_indexed | 2024-12-11T10:19:51Z |
| format | Article |
| id | doaj.art-f60176c7d5e848e381172d71bc4b8336 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T10:19:51Z |
| publishDate | 2019-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-f60176c7d5e848e381172d71bc4b83362022-12-22T01:11:29ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242019-04-0162010.1590/1678-4324-2019180178S1516-89132019000100401Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid contentVanessa Ghiggi SorgattoJulio Cesar de CarvalhoEduardo Bittencourt SydneyAdriane Bianchi Pedroni MedeirosLuciana Porto de Souza VandenbergheCarlos Ricardo SoccolAbstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401&lng=en&tlng=enmicrobial oilethanolysisFAEEmicroscalescreening |
| spellingShingle | Vanessa Ghiggi Sorgatto Julio Cesar de Carvalho Eduardo Bittencourt Sydney Adriane Bianchi Pedroni Medeiros Luciana Porto de Souza Vandenberghe Carlos Ricardo Soccol Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content Brazilian Archives of Biology and Technology microbial oil ethanolysis FAEE microscale screening |
| title | Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| title_full | Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| title_fullStr | Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| title_full_unstemmed | Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| title_short | Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| title_sort | microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content |
| topic | microbial oil ethanolysis FAEE microscale screening |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401&lng=en&tlng=en |
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