| Summary: | Background: Many research studies have investigated the antimicrobial activity of nanoparticles and herbal extracts on pathogenic bacteria. The aim of this study was to evaluate the antibacterial activity of silver nanoparticles in combination with L. angusifolia leaf extract against Escherichia coli and Staphylococcus aureus using response surface methodology.Methods: To evaluate the antibacterial activity of silver nanoparticles and L. angusifolia extract at different pH values against E.coli and S. aureus, the response surface methodology was used along with a central composite design. Agar well diffusion method was used to determine the antibacterial activity. Results: The results showed that the antibacterial activity of the combination of silver nanoparticles and L. angusifolia extract on E.coli (15.4 - 23.6 mm) was greater than that on S. aureus (11.7 – 21.6 mm). In addition, the antibacterial activity of the silver nanoparticles against E. coli and S. aureus was higher than that of L. angusifolia extract. The pH values had no effect on the antibacterial activity of the silver nanoparticles and L. angusifolia extract.Conclusion: The findings of this study showed that the combination of silver nanoparticles and L. angusifolia extract could be used as a possible source of effective antibacterial agent in infections.
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