The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia
Objectives: Pomegranate (Punica granatum L.) is frequently affected by fungal infections during the pre-and post-harvesting periods, resulting in severe losses to the farming economy. The common pre-and post-harvest pomegranate pathogens affecting the final yield and quality of the fruits are Botryt...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722003664 |
| _version_ | 1828198296328339456 |
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| author | Mohamed M. Hassan Mohamed M. Soliman Saad Al-Otaibi Ahmed M. El-Shehawi El-Kazafy A. Taha Samy Sayed |
| author_facet | Mohamed M. Hassan Mohamed M. Soliman Saad Al-Otaibi Ahmed M. El-Shehawi El-Kazafy A. Taha Samy Sayed |
| author_sort | Mohamed M. Hassan |
| collection | DOAJ |
| description | Objectives: Pomegranate (Punica granatum L.) is frequently affected by fungal infections during the pre-and post-harvesting periods, resulting in severe losses to the farming economy. The common pre-and post-harvest pomegranate pathogens affecting the final yield and quality of the fruits are Botrytis cinerea, Alternaria alternata, Penicillium implicatum, and Aspergillus niger. This study aimed to isolate and identify the pathogens causing pomegranate rot diseases. Also, the values of Xanthium strumarium extracts as eco-friendly control agents and three Trichoderma strains as biological control agents against the pomegranate fungal pathogen were determined. Methods: The plate dilution method was used to isolate fungi populations from pomegranate plants. The pathogens fungi were identified by the morphological and molecular method by sequenced of internal transcript spacer (ITS) region. The antifungal activities of X. strumarium extracted either by both ethanol or methanol and Trichoderma were investigated. Results and conclusions: The isolated and identified pathogens were P. implicatum, A. alternata, A. niger, Fusarium oxysporum, and F. chlamydosporum. The obtained sequences of these fungi were deposited in NCBI gene bank with accession numbers OK562113–OK562125. The efficiency of X. strumarium extract against pomegranate fungal pathogens ranged from 40.32 to 69.53% for ethanol extract and from 44.40 to 70.28% for methanol extract compared to 44.43%–85.71% for the antifungal Nystatin. Moreover, the efficiency of Trichoderma was 42.22%–72.50%, 42.30%–70.21%, and 44.54%–72.50% for strains ABSA-16, TSA-17, and ABSA-18, respectively. It could be concluded that the isolated pomegranate's pathogenic fungi in Taif were P. implicatum, A. alternata, A. niger, F. oxysporum, and F. chlamydosporum. The extract of X. strumarium obtained from the Taif region and Trichoderma can successfully assist control the pomegranate's pathogenic fungi at pre-and post-harvest, and hence can be successfully included in the IPM programs. |
| first_indexed | 2024-04-12T10:35:23Z |
| format | Article |
| id | doaj.art-f60335277af54c1cbbc368f29ecf9151 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T10:35:23Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-f60335277af54c1cbbc368f29ecf91512022-12-22T03:36:45ZengElsevierJournal of King Saud University: Science1018-36472022-08-01346102185The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi ArabiaMohamed M. Hassan0Mohamed M. Soliman1Saad Al-Otaibi2Ahmed M. El-Shehawi3El-Kazafy A. Taha4Samy Sayed5Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia; Corresponding authors.Clinical Laboratory Sciences Department, Turabah University College, Taif University, 21995, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaEconomic Entomology Department, College of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt; Corresponding authors.Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi ArabiaObjectives: Pomegranate (Punica granatum L.) is frequently affected by fungal infections during the pre-and post-harvesting periods, resulting in severe losses to the farming economy. The common pre-and post-harvest pomegranate pathogens affecting the final yield and quality of the fruits are Botrytis cinerea, Alternaria alternata, Penicillium implicatum, and Aspergillus niger. This study aimed to isolate and identify the pathogens causing pomegranate rot diseases. Also, the values of Xanthium strumarium extracts as eco-friendly control agents and three Trichoderma strains as biological control agents against the pomegranate fungal pathogen were determined. Methods: The plate dilution method was used to isolate fungi populations from pomegranate plants. The pathogens fungi were identified by the morphological and molecular method by sequenced of internal transcript spacer (ITS) region. The antifungal activities of X. strumarium extracted either by both ethanol or methanol and Trichoderma were investigated. Results and conclusions: The isolated and identified pathogens were P. implicatum, A. alternata, A. niger, Fusarium oxysporum, and F. chlamydosporum. The obtained sequences of these fungi were deposited in NCBI gene bank with accession numbers OK562113–OK562125. The efficiency of X. strumarium extract against pomegranate fungal pathogens ranged from 40.32 to 69.53% for ethanol extract and from 44.40 to 70.28% for methanol extract compared to 44.43%–85.71% for the antifungal Nystatin. Moreover, the efficiency of Trichoderma was 42.22%–72.50%, 42.30%–70.21%, and 44.54%–72.50% for strains ABSA-16, TSA-17, and ABSA-18, respectively. It could be concluded that the isolated pomegranate's pathogenic fungi in Taif were P. implicatum, A. alternata, A. niger, F. oxysporum, and F. chlamydosporum. The extract of X. strumarium obtained from the Taif region and Trichoderma can successfully assist control the pomegranate's pathogenic fungi at pre-and post-harvest, and hence can be successfully included in the IPM programs.http://www.sciencedirect.com/science/article/pii/S1018364722003664Internal transcript spacerPathogensPomegranateTaifTrichodermaXanthium strumarium |
| spellingShingle | Mohamed M. Hassan Mohamed M. Soliman Saad Al-Otaibi Ahmed M. El-Shehawi El-Kazafy A. Taha Samy Sayed The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia Journal of King Saud University: Science Internal transcript spacer Pathogens Pomegranate Taif Trichoderma Xanthium strumarium |
| title | The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia |
| title_full | The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia |
| title_fullStr | The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia |
| title_full_unstemmed | The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia |
| title_short | The effectiveness of Xanthium strumamrium L. extract and Trichoderma spp. against pomegranate isolated pathogenic fungi in Taif, Saudi Arabia |
| title_sort | effectiveness of xanthium strumamrium l extract and trichoderma spp against pomegranate isolated pathogenic fungi in taif saudi arabia |
| topic | Internal transcript spacer Pathogens Pomegranate Taif Trichoderma Xanthium strumarium |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722003664 |
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