Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p
AbstractBackground Long non-coding RNAs (lncRNAs) have been widely used in the exploration of diseases in recent years. This paper introduced the significance of lncRNA LINC00324 (LINC00324) on the progression of cervical cancer and explored the mechanism of action and potential prognosis of LINC003...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Journal of Obstetrics and Gynaecology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2023.2285384 |
| _version_ | 1797401586680463360 |
|---|---|
| author | Suna Ni Zhixia Wei Dandan Li |
| author_facet | Suna Ni Zhixia Wei Dandan Li |
| author_sort | Suna Ni |
| collection | DOAJ |
| description | AbstractBackground Long non-coding RNAs (lncRNAs) have been widely used in the exploration of diseases in recent years. This paper introduced the significance of lncRNA LINC00324 (LINC00324) on the progression of cervical cancer and explored the mechanism of action and potential prognosis of LINC00324.Methods The cervical cancer tissues and adjacent normal tissues of 120 people were collected as research samples. The expression level of LINC00324 was assessed by RT-qPCR, as was miR-195-5p. Knockdown of LINC00324 on the proliferation ability of cervical cancer cells was determined with the help of cell counting kit-8 (CCK-8), and the number of cell migration and invasion was detected by the Transwell method. Luciferase reporter gene assay was used to analyse the correlation of LINC00324 and miR-195-5p. Kaplan–Meier survival curves and multivariate Cox analysis explained the potential prognostic significance of LINC00324 in cervical cancer.Results Significantly increased expression of LINC00324 and down-regulated miR-195-5p were negatively correlated in cervical cancer. Knockdown of LINC00324 inhibited the progression of cervical cancer, which was related to its mechanism of targeting and downregulating miR-195-5p. In addition, low expression of LINC00324 may prolong the survival period of patients with cervical cancer.Conclusions LINC00324 targets miR-195-5p to regulate the progression of cervical cancer and have potential as a prognostic molecular marker for cervical cancer. |
| first_indexed | 2024-03-09T02:12:16Z |
| format | Article |
| id | doaj.art-f60420ad546744e0a36804e9dedfe5e1 |
| institution | Directory Open Access Journal |
| issn | 0144-3615 1364-6893 |
| language | English |
| last_indexed | 2024-03-09T02:12:16Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj.art-f60420ad546744e0a36804e9dedfe5e12023-12-07T10:34:37ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932023-12-0143210.1080/01443615.2023.2285384Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5pSuna Ni0Zhixia Wei1Dandan Li2Department of Obstetrics Clinic, ShiJiaZhuang Maternity & Child Healthcare Hospital, ShiJiaZhuang, ChinaDepartment of Obstetrics Clinic, ShiJiaZhuang Maternity & Child Healthcare Hospital, ShiJiaZhuang, ChinaDepartment of Breast Clinic, ShiJiaZhuang Maternity & Child Healthcare Hospital, ShiJiaZhuang, ChinaAbstractBackground Long non-coding RNAs (lncRNAs) have been widely used in the exploration of diseases in recent years. This paper introduced the significance of lncRNA LINC00324 (LINC00324) on the progression of cervical cancer and explored the mechanism of action and potential prognosis of LINC00324.Methods The cervical cancer tissues and adjacent normal tissues of 120 people were collected as research samples. The expression level of LINC00324 was assessed by RT-qPCR, as was miR-195-5p. Knockdown of LINC00324 on the proliferation ability of cervical cancer cells was determined with the help of cell counting kit-8 (CCK-8), and the number of cell migration and invasion was detected by the Transwell method. Luciferase reporter gene assay was used to analyse the correlation of LINC00324 and miR-195-5p. Kaplan–Meier survival curves and multivariate Cox analysis explained the potential prognostic significance of LINC00324 in cervical cancer.Results Significantly increased expression of LINC00324 and down-regulated miR-195-5p were negatively correlated in cervical cancer. Knockdown of LINC00324 inhibited the progression of cervical cancer, which was related to its mechanism of targeting and downregulating miR-195-5p. In addition, low expression of LINC00324 may prolong the survival period of patients with cervical cancer.Conclusions LINC00324 targets miR-195-5p to regulate the progression of cervical cancer and have potential as a prognostic molecular marker for cervical cancer.https://www.tandfonline.com/doi/10.1080/01443615.2023.2285384lncRNA LINC00324cervical cancermiR-195-5p |
| spellingShingle | Suna Ni Zhixia Wei Dandan Li Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p Journal of Obstetrics and Gynaecology lncRNA LINC00324 cervical cancer miR-195-5p |
| title | Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p |
| title_full | Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p |
| title_fullStr | Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p |
| title_full_unstemmed | Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p |
| title_short | Effect of lncRNA LINC00324 on cervical cancer progression through down-regulation of miR-195-5p |
| title_sort | effect of lncrna linc00324 on cervical cancer progression through down regulation of mir 195 5p |
| topic | lncRNA LINC00324 cervical cancer miR-195-5p |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2023.2285384 |
| work_keys_str_mv | AT sunani effectoflncrnalinc00324oncervicalcancerprogressionthroughdownregulationofmir1955p AT zhixiawei effectoflncrnalinc00324oncervicalcancerprogressionthroughdownregulationofmir1955p AT dandanli effectoflncrnalinc00324oncervicalcancerprogressionthroughdownregulationofmir1955p |