MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species.
Dengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are p...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0276488 |
| _version_ | 1811329556702822400 |
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| author | Antsa Rakotonirina Morgane Pol Fara Nantenaina Raharimalala Valentine Ballan Malia Kainiu Sébastien Boyer Sosiasi Kilama Sébastien Marcombe Sylvie Russet Emilie Barsac Rama Vineshwaran Malia Kaleméli Selemago Vincent Jessop Geneviève Robic Romain Girod Paul T Brey Julien Colot Myrielle Dupont-Rouzeyrol Vincent Richard Nicolas Pocquet |
| author_facet | Antsa Rakotonirina Morgane Pol Fara Nantenaina Raharimalala Valentine Ballan Malia Kainiu Sébastien Boyer Sosiasi Kilama Sébastien Marcombe Sylvie Russet Emilie Barsac Rama Vineshwaran Malia Kaleméli Selemago Vincent Jessop Geneviève Robic Romain Girod Paul T Brey Julien Colot Myrielle Dupont-Rouzeyrol Vincent Richard Nicolas Pocquet |
| author_sort | Antsa Rakotonirina |
| collection | DOAJ |
| description | Dengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are present. Due to the expansion of human travel and international trade, the threat of their dispersal in other world regions is on the rise. Strengthening of entomological surveillance ensuring rapid detection of introduced vector species is therefore required in order to avoid their establishment and the risk of arbovirus outbreaks. This surveillance relies on accurate species identification. The aim of this study was to assess the use of the Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) as a tool for an international identification and surveillance of these mosquito vectors of arboviruses. Field-mosquitoes belonging to 8 species (Ae. aegypti, Ae. albopictus, Aedes polynesiensis, Aedes scutellaris, Aedes pseudoscutellaris, Aedes malayensis, Aedes futunae and Culex quinquefasciatus) from 6 countries in the Pacific, Asian and Madagascar, were included in this study. Analysis provided evidence that a MALDI-TOF database created using mosquitoes from the Pacific region allowed suitable identification of mosquito species from the other regions. This technic was as efficient as the DNA sequencing method in identifying mosquito species. Indeed, with the exception of two Ae. pseudoscutellaris, an exact species identification was obtained for all individual mosquitoes. These findings highlight that the MALDI-TOF MS is a promising tool that could be used for a global comprehensive arbovirus vector surveillance. |
| first_indexed | 2024-04-13T15:45:59Z |
| format | Article |
| id | doaj.art-f604786dab544cb480c9d2f9d71082f4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T15:45:59Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f604786dab544cb480c9d2f9d71082f42022-12-22T02:40:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710e027648810.1371/journal.pone.0276488MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species.Antsa RakotonirinaMorgane PolFara Nantenaina RaharimalalaValentine BallanMalia KainiuSébastien BoyerSosiasi KilamaSébastien MarcombeSylvie RussetEmilie BarsacRama VineshwaranMalia Kaleméli SelemagoVincent JessopGeneviève RobicRomain GirodPaul T BreyJulien ColotMyrielle Dupont-RouzeyrolVincent RichardNicolas PocquetDengue, Zika and chikungunya viruses cause significant human public health burdens in the world. These arboviruses are transmitted by vector mosquito species notably Aedes aegypti and Aedes albopictus. In the Pacific region, more vector species of arboviruses belonging to the Scutellaris Group are present. Due to the expansion of human travel and international trade, the threat of their dispersal in other world regions is on the rise. Strengthening of entomological surveillance ensuring rapid detection of introduced vector species is therefore required in order to avoid their establishment and the risk of arbovirus outbreaks. This surveillance relies on accurate species identification. The aim of this study was to assess the use of the Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) as a tool for an international identification and surveillance of these mosquito vectors of arboviruses. Field-mosquitoes belonging to 8 species (Ae. aegypti, Ae. albopictus, Aedes polynesiensis, Aedes scutellaris, Aedes pseudoscutellaris, Aedes malayensis, Aedes futunae and Culex quinquefasciatus) from 6 countries in the Pacific, Asian and Madagascar, were included in this study. Analysis provided evidence that a MALDI-TOF database created using mosquitoes from the Pacific region allowed suitable identification of mosquito species from the other regions. This technic was as efficient as the DNA sequencing method in identifying mosquito species. Indeed, with the exception of two Ae. pseudoscutellaris, an exact species identification was obtained for all individual mosquitoes. These findings highlight that the MALDI-TOF MS is a promising tool that could be used for a global comprehensive arbovirus vector surveillance.https://doi.org/10.1371/journal.pone.0276488 |
| spellingShingle | Antsa Rakotonirina Morgane Pol Fara Nantenaina Raharimalala Valentine Ballan Malia Kainiu Sébastien Boyer Sosiasi Kilama Sébastien Marcombe Sylvie Russet Emilie Barsac Rama Vineshwaran Malia Kaleméli Selemago Vincent Jessop Geneviève Robic Romain Girod Paul T Brey Julien Colot Myrielle Dupont-Rouzeyrol Vincent Richard Nicolas Pocquet MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. PLoS ONE |
| title | MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. |
| title_full | MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. |
| title_fullStr | MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. |
| title_full_unstemmed | MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. |
| title_short | MALDI-TOF MS: An effective tool for a global surveillance of dengue vector species. |
| title_sort | maldi tof ms an effective tool for a global surveillance of dengue vector species |
| url | https://doi.org/10.1371/journal.pone.0276488 |
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