Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates
Dogs are reservoirs of different <i>Staphylococcus</i> species, but at the same time, they could develop several clinical forms caused by these bacteria. The aim of the present investigation was to characterize 50 clinical <i>Staphylococcus</i> isolates cultured from sick dog...
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/3/515 |
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| author | Fabrizio Bertelloni Giulia Cagnoli Valentina Virginia Ebani |
| author_facet | Fabrizio Bertelloni Giulia Cagnoli Valentina Virginia Ebani |
| author_sort | Fabrizio Bertelloni |
| collection | DOAJ |
| description | Dogs are reservoirs of different <i>Staphylococcus</i> species, but at the same time, they could develop several clinical forms caused by these bacteria. The aim of the present investigation was to characterize 50 clinical <i>Staphylococcus</i> isolates cultured from sick dogs. Bacterial species determination, hemolysins, protease, lipase, gelatinase, slime, and biofilm production, presence of virulence genes (<i>lukS/F-PV</i>, <i>eta</i>, <i>etb</i>, <i>tsst</i>, <i>icaA</i>, and <i>icaD</i>), methicillin resistance, and antimicrobial resistance were investigated. Most isolates (52%) were <i>Staphylococcus pseudointermedius</i>, but 20% and 8% belonged to <i>Staphylococcus</i><i>xylosus</i> and <i>Staphylococcus chromogenes</i>, respectively. Gelatinase, biofilm, and slime production were very common characters among the investigated strains with 80%, 86%, and 76% positive isolates, respectively. Virulence genes were detected in a very small number of the tested strains. A percentage of 14% of isolates were <i>mecA</i>-positive and phenotypically-resistant to methicillin. Multi-drug resistance was detected in 76% of tested staphylococci; in particular, high levels of resistance were detected for ampicillin, amoxicillin, clindamycin, and erythromycin. In conclusion, although staphylococci are considered to be opportunistic bacteria, the obtained data showed that dogs may be infected by <i>Staphylococcus</i> strains with important virulence characteristics and a high antimicrobial resistance. |
| first_indexed | 2024-03-09T05:54:51Z |
| format | Article |
| id | doaj.art-f6057d9cc9934f78909d3bf366a7ac9e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T05:54:51Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f6057d9cc9934f78909d3bf366a7ac9e2023-12-03T12:14:57ZengMDPI AGMicroorganisms2076-26072021-03-019351510.3390/microorganisms9030515Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. IsolatesFabrizio Bertelloni0Giulia Cagnoli1Valentina Virginia Ebani2Department of Veterinary Sciences, University of Pisa, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, 56124 Pisa, ItalyDogs are reservoirs of different <i>Staphylococcus</i> species, but at the same time, they could develop several clinical forms caused by these bacteria. The aim of the present investigation was to characterize 50 clinical <i>Staphylococcus</i> isolates cultured from sick dogs. Bacterial species determination, hemolysins, protease, lipase, gelatinase, slime, and biofilm production, presence of virulence genes (<i>lukS/F-PV</i>, <i>eta</i>, <i>etb</i>, <i>tsst</i>, <i>icaA</i>, and <i>icaD</i>), methicillin resistance, and antimicrobial resistance were investigated. Most isolates (52%) were <i>Staphylococcus pseudointermedius</i>, but 20% and 8% belonged to <i>Staphylococcus</i><i>xylosus</i> and <i>Staphylococcus chromogenes</i>, respectively. Gelatinase, biofilm, and slime production were very common characters among the investigated strains with 80%, 86%, and 76% positive isolates, respectively. Virulence genes were detected in a very small number of the tested strains. A percentage of 14% of isolates were <i>mecA</i>-positive and phenotypically-resistant to methicillin. Multi-drug resistance was detected in 76% of tested staphylococci; in particular, high levels of resistance were detected for ampicillin, amoxicillin, clindamycin, and erythromycin. In conclusion, although staphylococci are considered to be opportunistic bacteria, the obtained data showed that dogs may be infected by <i>Staphylococcus</i> strains with important virulence characteristics and a high antimicrobial resistance.https://www.mdpi.com/2076-2607/9/3/515<i>Staphylococcus</i>dogantimicrobial resistancevirulencebiofilm |
| spellingShingle | Fabrizio Bertelloni Giulia Cagnoli Valentina Virginia Ebani Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates Microorganisms <i>Staphylococcus</i> dog antimicrobial resistance virulence biofilm |
| title | Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates |
| title_full | Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates |
| title_fullStr | Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates |
| title_full_unstemmed | Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates |
| title_short | Virulence and Antimicrobial Resistance in Canine <i>Staphylococcus</i> spp. Isolates |
| title_sort | virulence and antimicrobial resistance in canine i staphylococcus i spp isolates |
| topic | <i>Staphylococcus</i> dog antimicrobial resistance virulence biofilm |
| url | https://www.mdpi.com/2076-2607/9/3/515 |
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