Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas
Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Allergy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/falgy.2021.691627/full |
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| author | Andrea Wangorsch Annette Jamin Stephanie Eichhorn Isabel Pablos Swati Sharma Bettina Schweidler Bianca Kastner Sabrina Wildner Joachim Saloga Frank Führer Reinaldo Rafael Reyna Orozco Roya Sherkat Somayeh Sadeghi Fardis Teifoori Jung-Won Park Peter Briza Stefan Vieths Fatima Ferreira Naveen Arora Jonas Lidholm Gabriele Gadermaier Stephan Scheurer |
| author_facet | Andrea Wangorsch Annette Jamin Stephanie Eichhorn Isabel Pablos Swati Sharma Bettina Schweidler Bianca Kastner Sabrina Wildner Joachim Saloga Frank Führer Reinaldo Rafael Reyna Orozco Roya Sherkat Somayeh Sadeghi Fardis Teifoori Jung-Won Park Peter Briza Stefan Vieths Fatima Ferreira Naveen Arora Jonas Lidholm Gabriele Gadermaier Stephan Scheurer |
| author_sort | Andrea Wangorsch |
| collection | DOAJ |
| description | Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD).Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy.Methods: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing.Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered.Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens. |
| first_indexed | 2024-12-18T20:56:29Z |
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| id | doaj.art-f607cf4b7067471ba120fea7e497b538 |
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| issn | 2673-6101 |
| language | English |
| last_indexed | 2024-12-18T20:56:29Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Allergy |
| spelling | doaj.art-f607cf4b7067471ba120fea7e497b5382022-12-21T20:53:16ZengFrontiers Media S.A.Frontiers in Allergy2673-61012021-06-01210.3389/falgy.2021.691627691627Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical AreasAndrea Wangorsch0Annette Jamin1Stephanie Eichhorn2Isabel Pablos3Swati Sharma4Bettina Schweidler5Bianca Kastner6Sabrina Wildner7Joachim Saloga8Frank Führer9Reinaldo Rafael Reyna Orozco10Roya Sherkat11Somayeh Sadeghi12Fardis Teifoori13Jung-Won Park14Peter Briza15Stefan Vieths16Fatima Ferreira17Naveen Arora18Jonas Lidholm19Gabriele Gadermaier20Stephan Scheurer21Molecular Allergology, Paul-Ehrlich-Institut, Langen, GermanyMolecular Allergology, Paul-Ehrlich-Institut, Langen, GermanyDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaAllergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, New Delhi, IndiaDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaDepartment of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, GermanyBatch Control and Allergen Analysis, Paul-Ehrlich-Institut, Langen, GermanyClinical Immunology Service in Hospital Militar Dr. Carlos Arvelo, Caracas, VenezuelaAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranAcquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Internal Medicine, Yonsei University College of Medicine, Seoul, South KoreaDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaMolecular Allergology, Paul-Ehrlich-Institut, Langen, GermanyDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaAllergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, New Delhi, IndiaThermo Fisher Scientific, Immunodiagnostics, Uppsala, SwedenDepartment of Biosciences, Paris Lodron University of Salzburg, Salzburg, AustriaMolecular Allergology, Paul-Ehrlich-Institut, Langen, GermanyBackground: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD).Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy.Methods: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing.Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered.Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens.https://www.frontiersin.org/articles/10.3389/falgy.2021.691627/fullcockroach allergyAmerican cockroachBlattella germanicacomponent-resolved diagnosisPeriplaneta americana (Insecta) |
| spellingShingle | Andrea Wangorsch Annette Jamin Stephanie Eichhorn Isabel Pablos Swati Sharma Bettina Schweidler Bianca Kastner Sabrina Wildner Joachim Saloga Frank Führer Reinaldo Rafael Reyna Orozco Roya Sherkat Somayeh Sadeghi Fardis Teifoori Jung-Won Park Peter Briza Stefan Vieths Fatima Ferreira Naveen Arora Jonas Lidholm Gabriele Gadermaier Stephan Scheurer Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas Frontiers in Allergy cockroach allergy American cockroach Blattella germanica component-resolved diagnosis Periplaneta americana (Insecta) |
| title | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
| title_full | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
| title_fullStr | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
| title_full_unstemmed | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
| title_short | Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas |
| title_sort | component resolved diagnosis of american cockroach periplaneta americana allergy in patients from different geographical areas |
| topic | cockroach allergy American cockroach Blattella germanica component-resolved diagnosis Periplaneta americana (Insecta) |
| url | https://www.frontiersin.org/articles/10.3389/falgy.2021.691627/full |
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