In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes
Impaired skeletal muscle growth in utero can result in reduced birth weight and poor carcass quality in pigs. Recently, we showed the role of microRNAs (miRNAs) and their target genes in prenatal skeletal muscle development and pathogenesis of intrauterine growth restriction (IUGR). In this study, w...
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MDPI AG
2021-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/8/1264 |
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| author | Asghar Ali Eduard Murani Frieder Hadlich Xuan Liu Klaus Wimmers Siriluck Ponsuksili |
| author_facet | Asghar Ali Eduard Murani Frieder Hadlich Xuan Liu Klaus Wimmers Siriluck Ponsuksili |
| author_sort | Asghar Ali |
| collection | DOAJ |
| description | Impaired skeletal muscle growth in utero can result in reduced birth weight and poor carcass quality in pigs. Recently, we showed the role of microRNAs (miRNAs) and their target genes in prenatal skeletal muscle development and pathogenesis of intrauterine growth restriction (IUGR). In this study, we performed an integrative miRNA-mRNA transcriptomic analysis in longissimus dorsi muscle (LDM) of pig fetuses at 63 days post conception (dpc) to identify miRNAs and genes correlated to fetal weight. We found 13 miRNAs in LDM significantly correlated to fetal weight, including miR-140, miR-186, miR-101, miR-15, miR-24, miR-29, miR-449, miR-27, miR-142, miR-99, miR-181, miR-199, and miR-210. The expression of these miRNAs decreased with an increase in fetal weight. We also identified 1315 genes significantly correlated to fetal weight at 63 dpc, of which 135 genes were negatively correlated as well as identified as potential targets of the above-listed 13 miRNAs. These miRNAs and their target genes enriched pathways and biological processes important for fetal growth, development, and metabolism. These results indicate that the transcriptomic profile of skeletal muscle can be used to predict fetal weight, and miRNAs correlated to fetal weight can serve as potential biomarkers of prenatal fetal health and growth. |
| first_indexed | 2024-03-10T08:47:20Z |
| format | Article |
| id | doaj.art-f6099af4894445bdab273e2e3cd07059 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T08:47:20Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-f6099af4894445bdab273e2e3cd070592023-11-22T07:46:53ZengMDPI AGGenes2073-44252021-08-01128126410.3390/genes12081264In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target GenesAsghar Ali0Eduard Murani1Frieder Hadlich2Xuan Liu3Klaus Wimmers4Siriluck Ponsuksili5Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyImpaired skeletal muscle growth in utero can result in reduced birth weight and poor carcass quality in pigs. Recently, we showed the role of microRNAs (miRNAs) and their target genes in prenatal skeletal muscle development and pathogenesis of intrauterine growth restriction (IUGR). In this study, we performed an integrative miRNA-mRNA transcriptomic analysis in longissimus dorsi muscle (LDM) of pig fetuses at 63 days post conception (dpc) to identify miRNAs and genes correlated to fetal weight. We found 13 miRNAs in LDM significantly correlated to fetal weight, including miR-140, miR-186, miR-101, miR-15, miR-24, miR-29, miR-449, miR-27, miR-142, miR-99, miR-181, miR-199, and miR-210. The expression of these miRNAs decreased with an increase in fetal weight. We also identified 1315 genes significantly correlated to fetal weight at 63 dpc, of which 135 genes were negatively correlated as well as identified as potential targets of the above-listed 13 miRNAs. These miRNAs and their target genes enriched pathways and biological processes important for fetal growth, development, and metabolism. These results indicate that the transcriptomic profile of skeletal muscle can be used to predict fetal weight, and miRNAs correlated to fetal weight can serve as potential biomarkers of prenatal fetal health and growth.https://www.mdpi.com/2073-4425/12/8/1264fetal growthlongissimus dorsi muscleprenatal developmentskeletal muscleintrauterine growth |
| spellingShingle | Asghar Ali Eduard Murani Frieder Hadlich Xuan Liu Klaus Wimmers Siriluck Ponsuksili In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes Genes fetal growth longissimus dorsi muscle prenatal development skeletal muscle intrauterine growth |
| title | In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes |
| title_full | In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes |
| title_fullStr | In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes |
| title_full_unstemmed | In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes |
| title_short | In Utero Fetal Weight in Pigs Is Regulated by microRNAs and Their Target Genes |
| title_sort | in utero fetal weight in pigs is regulated by micrornas and their target genes |
| topic | fetal growth longissimus dorsi muscle prenatal development skeletal muscle intrauterine growth |
| url | https://www.mdpi.com/2073-4425/12/8/1264 |
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