Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal
In this study, mycelia of eight recently described species of <i>Halophytophthora</i> and <i>H. avicennae</i> collected in Southern Portugal were analysed for lipids and fatty acids (FA) content to evaluate their possible use as alternative sources of FAs and understand how e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/4/227 |
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| author | Cristiana Maia Thomas Jung Aschwin Engelen Marília Horta Jung Luísa Custódio |
| author_facet | Cristiana Maia Thomas Jung Aschwin Engelen Marília Horta Jung Luísa Custódio |
| author_sort | Cristiana Maia |
| collection | DOAJ |
| description | In this study, mycelia of eight recently described species of <i>Halophytophthora</i> and <i>H. avicennae</i> collected in Southern Portugal were analysed for lipids and fatty acids (FA) content to evaluate their possible use as alternative sources of FAs and understand how each species FAs profile relates to their phylogenetic position. All species had a low lipid percentage (0.06% in <i>H. avicennae</i> to 0.28% in <i>H. frigida</i>). Subclade 6b species contained more lipids. All species produced monounsaturated (MUFA), polyunsaturated (PUFA) and saturated (SFA) FAs, the latter being most abundant in all species. <i>H. avicennae</i> had the highest FA variety and was the only producer of γ-linolenic acid, while <i>H. brevisporangia</i> produced the lowest number of FAs. The best producer of arachidonic acid (ARA) and eicosapentaenoic acid (EPA) was <i>H. thermoambigua</i> with 3.89% and 9.09% of total FAs, respectively. In all species, palmitic acid (SFA) was most abundant and among the MUFAs produced oleic acid had the highest relative percentage. Principal component analysis (PCA) showed partial segregation of species by phylogenetic clade and subclade based on their FA profile. <i>H. avicennae</i> (Clade 4) differed from all other Clade 6 species due to the production of γ-linolenic and lauric acids. Our results disclosed interesting FA profiles in the tested species, adequate for energy (biodiesel), pharmaceutical and food industries (bioactive FAs). Despite the low amounts of lipids produced, this can be boosted by manipulating culture growth conditions. The observed interspecific variations in FA production provide preliminary insights into an evolutionary background of its production. |
| first_indexed | 2024-03-11T04:49:37Z |
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| id | doaj.art-f60a93aabf7748118fbe679ea46bb549 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T04:49:37Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f60a93aabf7748118fbe679ea46bb5492023-11-17T20:10:16ZengMDPI AGMarine Drugs1660-33972023-03-0121422710.3390/md21040227Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of PortugalCristiana Maia0Thomas Jung1Aschwin Engelen2Marília Horta Jung3Luísa Custódio4Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, PortugalPhytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, 613 00 Brno, Czech RepublicCentre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, PortugalPhytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, 613 00 Brno, Czech RepublicCentre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, PortugalIn this study, mycelia of eight recently described species of <i>Halophytophthora</i> and <i>H. avicennae</i> collected in Southern Portugal were analysed for lipids and fatty acids (FA) content to evaluate their possible use as alternative sources of FAs and understand how each species FAs profile relates to their phylogenetic position. All species had a low lipid percentage (0.06% in <i>H. avicennae</i> to 0.28% in <i>H. frigida</i>). Subclade 6b species contained more lipids. All species produced monounsaturated (MUFA), polyunsaturated (PUFA) and saturated (SFA) FAs, the latter being most abundant in all species. <i>H. avicennae</i> had the highest FA variety and was the only producer of γ-linolenic acid, while <i>H. brevisporangia</i> produced the lowest number of FAs. The best producer of arachidonic acid (ARA) and eicosapentaenoic acid (EPA) was <i>H. thermoambigua</i> with 3.89% and 9.09% of total FAs, respectively. In all species, palmitic acid (SFA) was most abundant and among the MUFAs produced oleic acid had the highest relative percentage. Principal component analysis (PCA) showed partial segregation of species by phylogenetic clade and subclade based on their FA profile. <i>H. avicennae</i> (Clade 4) differed from all other Clade 6 species due to the production of γ-linolenic and lauric acids. Our results disclosed interesting FA profiles in the tested species, adequate for energy (biodiesel), pharmaceutical and food industries (bioactive FAs). Despite the low amounts of lipids produced, this can be boosted by manipulating culture growth conditions. The observed interspecific variations in FA production provide preliminary insights into an evolutionary background of its production.https://www.mdpi.com/1660-3397/21/4/227marine oomycetesFAMEslipidsmarine biotechnology |
| spellingShingle | Cristiana Maia Thomas Jung Aschwin Engelen Marília Horta Jung Luísa Custódio Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal Marine Drugs marine oomycetes FAMEs lipids marine biotechnology |
| title | Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal |
| title_full | Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal |
| title_fullStr | Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal |
| title_full_unstemmed | Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal |
| title_short | Unravelling the Lipids Content and the Fatty Acid Profiles of Eight Recently Described <i>Halophytophthora</i> Species and <i>H. avicennae</i> from the South Coast of Portugal |
| title_sort | unravelling the lipids content and the fatty acid profiles of eight recently described i halophytophthora i species and i h avicennae i from the south coast of portugal |
| topic | marine oomycetes FAMEs lipids marine biotechnology |
| url | https://www.mdpi.com/1660-3397/21/4/227 |
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