Mathematical modeling of dormant cell formation in growing biofilm
Understanding the dynamics of dormant cells in microbial biofilms, in which the bacteria are embedded in extracellular matrix, is important for developing successful antibiotic therapies against pathogenic bacteria. Although some of the molecular mechanisms leading to bacterial persistence have been...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00534/full |
| _version_ | 1819091294118477824 |
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| author | Kotaro eChihara Shinya eMatsumoto Yuki eKagawa Satoshi eTsuneda |
| author_facet | Kotaro eChihara Shinya eMatsumoto Yuki eKagawa Satoshi eTsuneda |
| author_sort | Kotaro eChihara |
| collection | DOAJ |
| description | Understanding the dynamics of dormant cells in microbial biofilms, in which the bacteria are embedded in extracellular matrix, is important for developing successful antibiotic therapies against pathogenic bacteria. Although some of the molecular mechanisms leading to bacterial persistence have been speculated in planktonic bacterial cell, how dormant cells emerge in the biofilms of pathogenic bacteria such as Pseudomonas aeruginosa remains unclear. The present study proposes four hypotheses of dormant cell formation; stochastic process, nutrient-dependent, oxygen-dependent, and time-dependent processes. These hypotheses were implemented into a three-dimensional individual-based model of biofilm formation. Numerical simulations of the different mechanisms yielded qualitatively different spatiotemporal distributions of dormant cells in the growing biofilm. Based on these simulation results, we discuss what kinds of experimental studies are effective for discriminating dormant cell formation mechanisms in biofilms. |
| first_indexed | 2024-12-21T22:37:26Z |
| format | Article |
| id | doaj.art-f60dd3983b1d469fad038affc89741bb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T22:37:26Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f60dd3983b1d469fad038affc89741bb2022-12-21T18:47:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-05-01610.3389/fmicb.2015.00534137760Mathematical modeling of dormant cell formation in growing biofilmKotaro eChihara0Shinya eMatsumoto1Yuki eKagawa2Satoshi eTsuneda3Waseda UniversityMontana State UniversityWaseda UniversityWaseda UniversityUnderstanding the dynamics of dormant cells in microbial biofilms, in which the bacteria are embedded in extracellular matrix, is important for developing successful antibiotic therapies against pathogenic bacteria. Although some of the molecular mechanisms leading to bacterial persistence have been speculated in planktonic bacterial cell, how dormant cells emerge in the biofilms of pathogenic bacteria such as Pseudomonas aeruginosa remains unclear. The present study proposes four hypotheses of dormant cell formation; stochastic process, nutrient-dependent, oxygen-dependent, and time-dependent processes. These hypotheses were implemented into a three-dimensional individual-based model of biofilm formation. Numerical simulations of the different mechanisms yielded qualitatively different spatiotemporal distributions of dormant cells in the growing biofilm. Based on these simulation results, we discuss what kinds of experimental studies are effective for discriminating dormant cell formation mechanisms in biofilms.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00534/fullBiofilmPersistencedormancymathematical modelingindividual-based modeling |
| spellingShingle | Kotaro eChihara Shinya eMatsumoto Yuki eKagawa Satoshi eTsuneda Mathematical modeling of dormant cell formation in growing biofilm Frontiers in Microbiology Biofilm Persistence dormancy mathematical modeling individual-based modeling |
| title | Mathematical modeling of dormant cell formation in growing biofilm |
| title_full | Mathematical modeling of dormant cell formation in growing biofilm |
| title_fullStr | Mathematical modeling of dormant cell formation in growing biofilm |
| title_full_unstemmed | Mathematical modeling of dormant cell formation in growing biofilm |
| title_short | Mathematical modeling of dormant cell formation in growing biofilm |
| title_sort | mathematical modeling of dormant cell formation in growing biofilm |
| topic | Biofilm Persistence dormancy mathematical modeling individual-based modeling |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00534/full |
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