Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken
ObjectiveChronic stress leads to a high circulating level of glucocorticoids, which disrupts lipid metabolism and causes non-alcoholic fatty liver disease in mice and humans. Meanwhile, bile acid (BA), a class of metabolites initially synthesized in the liver and further metabolized by gut microbiot...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1147024/full |
| _version_ | 1797825568307150848 |
|---|---|
| author | Lei Wu Xinyi Liu Aijia Zhang Huimin Chen Ruqian Zhao Yimin Jia Yimin Jia |
| author_facet | Lei Wu Xinyi Liu Aijia Zhang Huimin Chen Ruqian Zhao Yimin Jia Yimin Jia |
| author_sort | Lei Wu |
| collection | DOAJ |
| description | ObjectiveChronic stress leads to a high circulating level of glucocorticoids, which disrupts lipid metabolism and causes non-alcoholic fatty liver disease in mice and humans. Meanwhile, bile acid (BA), a class of metabolites initially synthesized in the liver and further metabolized by gut microbiota, plays a vital role in lipid metabolism. This study aimed to investigate the effects of glucocorticoids on BA metabolism and gut microbiota in chickens.MethodsIn this study, 35-day-old chickens were injected with 4 mg/kg/day corticosterone (Cort) for 14 days to simulate chronic stress.ResultsCort treatment significantly increased the triglyceride contents in the plasma and the liver. HE and oil-red staining showed that Cort treatment induced fatty liver in chickens. Meanwhile, Cort exposure downregulated total bile acid (TBA) content in the liver while increasing the TBA in feces. UPLC-HRMS results showed that Cort exposure significantly decreased the hepatic levels of CDCA, T-alpha-MCA, and T-beta-MCA. Moreover, Cort exposure significantly reduced the expression of genes related to BA synthesis (CYP8B1 and CYP27A1), conjugation (BACS), and regulation (KLβ and FGFR4). 16s sequencing results showed that Cort treatment significantly decreased the amount of Lachnospiraceae, Eisenbergiella, Blautia, and Eubacterium and increased the abundance of Barnesiella, Lactobacillus, and Helicobacter. Spearman correlation analysis showed a significant positive correlation between fecal TBA and the abundance of Lactobacillales, Lactobacillus, and Barnesiella. In comparison, TBA in the liver was positively correlated with Eubacterium, and negatively correlated with Helicobacter.ConclusionIn summary, chronic Cort exposure disrupts hepatic and intestinal bile acid metabolism inducing gut microbiome dysbiosis, which might associate with the development of fatty liver in chickens. |
| first_indexed | 2024-03-13T10:54:52Z |
| format | Article |
| id | doaj.art-f6125baf1ee84cf68faa728d36634a04 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T10:54:52Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-f6125baf1ee84cf68faa728d36634a042023-05-17T05:15:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-05-011010.3389/fvets.2023.11470241147024Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chickenLei Wu0Xinyi Liu1Aijia Zhang2Huimin Chen3Ruqian Zhao4Yimin Jia5Yimin Jia6Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaJiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing, ChinaObjectiveChronic stress leads to a high circulating level of glucocorticoids, which disrupts lipid metabolism and causes non-alcoholic fatty liver disease in mice and humans. Meanwhile, bile acid (BA), a class of metabolites initially synthesized in the liver and further metabolized by gut microbiota, plays a vital role in lipid metabolism. This study aimed to investigate the effects of glucocorticoids on BA metabolism and gut microbiota in chickens.MethodsIn this study, 35-day-old chickens were injected with 4 mg/kg/day corticosterone (Cort) for 14 days to simulate chronic stress.ResultsCort treatment significantly increased the triglyceride contents in the plasma and the liver. HE and oil-red staining showed that Cort treatment induced fatty liver in chickens. Meanwhile, Cort exposure downregulated total bile acid (TBA) content in the liver while increasing the TBA in feces. UPLC-HRMS results showed that Cort exposure significantly decreased the hepatic levels of CDCA, T-alpha-MCA, and T-beta-MCA. Moreover, Cort exposure significantly reduced the expression of genes related to BA synthesis (CYP8B1 and CYP27A1), conjugation (BACS), and regulation (KLβ and FGFR4). 16s sequencing results showed that Cort treatment significantly decreased the amount of Lachnospiraceae, Eisenbergiella, Blautia, and Eubacterium and increased the abundance of Barnesiella, Lactobacillus, and Helicobacter. Spearman correlation analysis showed a significant positive correlation between fecal TBA and the abundance of Lactobacillales, Lactobacillus, and Barnesiella. In comparison, TBA in the liver was positively correlated with Eubacterium, and negatively correlated with Helicobacter.ConclusionIn summary, chronic Cort exposure disrupts hepatic and intestinal bile acid metabolism inducing gut microbiome dysbiosis, which might associate with the development of fatty liver in chickens.https://www.frontiersin.org/articles/10.3389/fvets.2023.1147024/fullchronic stressglucocorticoidsfatty liverbile acidgut microbiota |
| spellingShingle | Lei Wu Xinyi Liu Aijia Zhang Huimin Chen Ruqian Zhao Yimin Jia Yimin Jia Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken Frontiers in Veterinary Science chronic stress glucocorticoids fatty liver bile acid gut microbiota |
| title | Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| title_full | Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| title_fullStr | Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| title_full_unstemmed | Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| title_short | Chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| title_sort | chronic corticosterone exposure disrupts hepatic and intestinal bile acid metabolism in chicken |
| topic | chronic stress glucocorticoids fatty liver bile acid gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1147024/full |
| work_keys_str_mv | AT leiwu chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT xinyiliu chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT aijiazhang chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT huiminchen chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT ruqianzhao chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT yiminjia chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken AT yiminjia chroniccorticosteroneexposuredisruptshepaticandintestinalbileacidmetabolisminchicken |