V5 and GFP Tagging of Viral Gene pp38 of Marek’s Disease Vaccine Strain CVI988 Using CRISPR/Cas9 Editing

V5 and GFP Tagging of Viral Gene pp38 of Marek’s Disease Vaccine Strain CVI988 Using CRISPR/Cas9 Editing

Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. The availability of the complete sequence of the viral genome allowed for the identification of major genes associated with pathogenicity using different...

Full description

Bibliographic Details
Main Authors:	Weicheng Li, Yaoyao Zhang, Katy Moffat, Venugopal Nair, Yongxiu Yao
Format:	Article
Language:	English
Published:	MDPI AG 2022-02-01
Series:	Viruses
Subjects:	CRISPR/Cas9 MDV pp38 tagging V5 GFP
Online Access:	https://www.mdpi.com/1999-4915/14/2/436

Similar Items

Targeted Editing of the <i>pp38</i> Gene in Marek’s Disease Virus-Transformed Cell Lines Using CRISPR/Cas9 System
by: Yaoyao Zhang, et al.
Published: (2019-04-01)

CRISPR-Mediated Gene Activation (CRISPRa) of pp38/pp24 Orchestrates Events Triggering Lytic Infection in Marek’s Disease Virus-Transformed Cell Lines
by: Poornima Roy, et al.
Published: (2021-08-01)

Pervasive Differential Splicing in Marek’s Disease Virus Can Discriminate CVI-988 Vaccine Strain from RB-1B Very Virulent Strain in Chicken Embryonic Fibroblasts
by: Yashar Sadigh, et al.
Published: (2020-03-01)

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology
by: Man Teng, et al.
Published: (2022-09-01)

First report of Serotype-1 Marek’s disease virus (MDV-1) with oncogenic form in backyard turkeys in Turkey: a molecular analysis study
by: Hasan Ongor, et al.
Published: (2022-01-01)

Use and Evaluation of a pES213-Derived Plasmid for the Constitutive Expression of gfp Protein in Pathogenic Vibrios: a Tagging Tool for In Vitro Studies
by: William A. Norfolk, et al.
Published: (2023-02-01)

Viral miRNA delivered by exosomes from Marek's disease virus-transformed lymphoma cell line exerts regulatory function in internalized primary chicken embryo fibroblast cells
by: Man Teng, et al.
Published: (2024-12-01)

Efficient Cross-Screening and Characterization of Monoclonal Antibodies against Marek’s Disease Specific Meq Oncoprotein Using CRISPR/Cas9-Gene-Edited Viruses
by: Man Teng, et al.
Published: (2023-03-01)

Pathogenicity and Pathotype Analysis of Henan Isolates of Marek’s Disease Virus Reveal Long-Term Circulation of Highly Virulent MDV Variant in China
by: Man Teng, et al.
Published: (2022-07-01)

Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline
by: Adam Hefel, et al.
Published: (2019-06-01)

Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function
by: Yaoyao Zhang, et al.
Published: (2018-05-01)

Fully Attenuated meq and pp38 Double Gene Deletion Mutant Virus Confers Superior Immunological Protection against Highly Virulent Marek’s Disease Virus Infection
by: Aijun Sun, et al.
Published: (2022-12-01)

Transcripts of antibacterial peptides in chicken erythrocytes infected with Marek’s disease virus
by: Sheng Niu, et al.
Published: (2018-11-01)

Emerging Hypervirulent Marek’s Disease Virus Variants Significantly Overcome Protection Conferred by Commercial Vaccines
by: Jin-Ling Liu, et al.
Published: (2023-06-01)

GFP markers for studying D. melanogaster spermatogenesis
by: Nerusheva Olga, et al.
Published: (2009-12-01)

An Efficient <i>Agrobacterium</i> Mediated Transformation of Pineapple with GFP-Tagged Protein Allows Easy, Non-Destructive Screening of Transgenic Pineapple Plants
by: S. V. G. N. Priyadarshani, et al.
Published: (2019-10-01)

RIOK3-Mediated Akt phosphorylation facilitates synergistic replication of Marek’s disease and reticuloendotheliosis viruses
by: Xusheng Du, et al.
Published: (2022-12-01)

Extreme thermal stability of the antiGFP nanobody – GFP complex
by: Balázs Kakasi, et al.
Published: (2023-06-01)

Sodium butyrate impedes the lymphoma caused by Marek’s disease virus via regulating the mitochondrial apoptosis pathway
by: Qiaoer Lin, et al.
Published: (2024-02-01)

Induction of lymphomas by inoculation of Marek's disease virus-derived lymphoblastoid cell lines: prevention by CVI988 vaccination.
by: Mwangi, W, et al.
Published: (2012)

A genetic toolkit for tagging intronic MiMIC containing genes
by: Sonal Nagarkar-Jaiswal, et al.
Published: (2015-06-01)

Dose-dependent Toxicity of Humanized Renilla reniformis GFP (hrGFP) Limits Its Utility as a Reporter Gene in Mouse Muscle
by: Lindsay M Wallace, et al.
Published: (2013-01-01)

Role of Virus-Encoded microRNAs in Avian Viral Diseases
by: Yongxiu Yao, et al.
Published: (2014-03-01)

Identification of the Receptor and Cellular Ortholog of the Marek's Disease Virus (MDV) CXC Chemokine
by: Sonja Haertle, et al.
Published: (2017-12-01)

The effect of Tea Misletoe (Scurrula oortiana) Stem Extract as Immuno-Modulator on Oncogenic Marek’s Disease Virus Infection
by: Mulyoto Samsi, et al.
Published: (2007-11-01)

Multifunctional miR-155 Pathway in Avian Oncogenic Virus-Induced Neoplastic Diseases
by: Megha Sravani Bondada, et al.
Published: (2019-03-01)

Identification of Disalicyloyl Curcumin as a Potential DNA Polymerase Inhibitor for Marek’s Disease Herpesvirus: A Computational Study Using Virtual Screening and Molecular Dynamics Simulations
by: Aziza Cherif, et al.
Published: (2023-09-01)

CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast
by: Lisa K. Berry, et al.
Published: (2020-03-01)

Protection Conferred by Gallid Alphaherpesvirus 2 Vaccines Against Immunosuppression Induced by Very Virulent Plus (vv+) Marek’s Disease Virus Strains in Commercial Meat Type Chickens
by: Nagwa Khaled, et al.
Published: (2025-01-01)

A novel protein-coding ORF72.2 gene was identified from Marek's disease virus strain CVI988
by: Liu Ping, et al.
Published: (2010-12-01)

N-Terminal Tagging with GFP Enhances Selectivity of Agitoxin 2 to Kv1.3-Channel Binding Site
by: Oksana V. Nekrasova, et al.
Published: (2020-12-01)

Optimizing protocols for monitoring in vivo replication of a novel chimeric Marek’s Disease vaccine with an insertion of the Long Terminal Repeat of Reticuloendotheliosis Virus in the CVI988 Strain genome (CVI-LTR)
by: Faiz, Nik M., et al.
Published: (2024)

Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Marek’s Disease Virus of GX0101 ∆ LTR
by: X Li, et al.

A Cell Culture System to Investigate Marek’s Disease Virus Integration into Host Chromosomes
by: Yu You, et al.
Published: (2021-12-01)

The Meq Gene Molecular Profile of Marek’s Disease Virus Serotype 1 From Kampung and Arabic Chicken Farms in Sukabumi, West Java, Indonesia
by: Risza Hartawan, et al.
Published: (2016-10-01)

Temporal Dynamics of Purinergic Receptor Expression in the Lungs of Marek’s Disease (MD) Virus-Infected Chickens Resistant or Susceptible to MD
by: Haji Akbar, et al.
Published: (2024-07-01)

One-step split GFP staining for sensitive protein detection and localization in mammalian cells
by: Lara Kaddoum, et al.
Published: (2010-10-01)

Toxicity of nuclear-localized GFP in reporter mice
by: Sudhir Verma, et al.
Published: (2024-10-01)

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
by: Li Wang, et al.
Published: (2021-03-01)

In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria
by: Elrike Frenzel, et al.
Published: (2018-01-01)