Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line
Background: Renal cell carcinoma is considered to be a radioresistant tumor. Consequently, radiotherapy is used mainly for palliation of metastases or local tumor growth. The aim of this study was to evaluate the efficacy of the radiation protocols with small fractions on cell killing of ACHN human...
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Isfahan University of Medical Sciences
2015-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/4724 |
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| author | Maryam Khorramizadeh Seyed Ali Hosein Saberi Parvaneh Shokrani Mohammad Javad Tahmasebi-Birgani Seyed Hosein Hejazi Ali Reza Amouhedari |
| author_facet | Maryam Khorramizadeh Seyed Ali Hosein Saberi Parvaneh Shokrani Mohammad Javad Tahmasebi-Birgani Seyed Hosein Hejazi Ali Reza Amouhedari |
| author_sort | Maryam Khorramizadeh |
| collection | DOAJ |
| description | Background: Renal cell carcinoma is considered to be a radioresistant tumor. Consequently, radiotherapy is used mainly for palliation of metastases or local tumor growth. The aim of this study was to evaluate the efficacy of the radiation protocols with small fractions on cell killing of ACHN human renal cell carcinoma cell line.
Methods: After cell culture, the ACHN cells were exposed to 6, 8 and 10 Gy of single fraction and 3 × 2, 4 × 2 and 5 × 2 Gy of two fractions. Viability was calculated using MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium].
Findings: Exposing cultured cells to small fractions of radiation resulted to a mean decrease of 70% in cell survival over 120 hours after radiation. Survival fraction reduced with increasing the dose per fraction. Two fractions 5 × 2 Gy showed significant effect on cell survival compared to single fraction 10 Gy (P = 0.02)
Conclusion: Our results indicate that the small fraction is a valuable alternative to provide local tumor control. |
| first_indexed | 2024-03-12T08:18:30Z |
| format | Article |
| id | doaj.art-f61755ed30db466bb50994a8dda4b0cd |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:18:30Z |
| publishDate | 2015-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-f61755ed30db466bb50994a8dda4b0cd2023-09-02T18:39:48ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2015-05-01333284004091845Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell LineMaryam Khorramizadeh0Seyed Ali Hosein Saberi1Parvaneh Shokrani2Mohammad Javad Tahmasebi-Birgani3Seyed Hosein Hejazi4Ali Reza Amouhedari5Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAssociate Professor, Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranProfessor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranProfessor, Skin Disease and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranRadiation Oncologist, Milad Hospital, Isfahan, IranBackground: Renal cell carcinoma is considered to be a radioresistant tumor. Consequently, radiotherapy is used mainly for palliation of metastases or local tumor growth. The aim of this study was to evaluate the efficacy of the radiation protocols with small fractions on cell killing of ACHN human renal cell carcinoma cell line. Methods: After cell culture, the ACHN cells were exposed to 6, 8 and 10 Gy of single fraction and 3 × 2, 4 × 2 and 5 × 2 Gy of two fractions. Viability was calculated using MTS assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. Findings: Exposing cultured cells to small fractions of radiation resulted to a mean decrease of 70% in cell survival over 120 hours after radiation. Survival fraction reduced with increasing the dose per fraction. Two fractions 5 × 2 Gy showed significant effect on cell survival compared to single fraction 10 Gy (P = 0.02) Conclusion: Our results indicate that the small fraction is a valuable alternative to provide local tumor control.http://jims.mui.ac.ir/index.php/jims/article/view/4724RadiotherapySmall radiation fractionationRenal cell carcinoma |
| spellingShingle | Maryam Khorramizadeh Seyed Ali Hosein Saberi Parvaneh Shokrani Mohammad Javad Tahmasebi-Birgani Seyed Hosein Hejazi Ali Reza Amouhedari Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line مجله دانشکده پزشکی اصفهان Radiotherapy Small radiation fractionation Renal cell carcinoma |
| title | Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line |
| title_full | Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line |
| title_fullStr | Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line |
| title_full_unstemmed | Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line |
| title_short | Evaluating the Effectiveness of Small Fractionation Radiotherapy Regimens on the ACHN Renal Cell Carcinoma Cell Line |
| title_sort | evaluating the effectiveness of small fractionation radiotherapy regimens on the achn renal cell carcinoma cell line |
| topic | Radiotherapy Small radiation fractionation Renal cell carcinoma |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/4724 |
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