Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells
Isocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechani...
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MDPI AG
2024-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/1/42 |
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| author | Qiaozhen Zhou Qianqian Zhang Lingzi Liao Qian Li Huidan Qu Xinyu Wang Ying Zhou Guangzeng Zhang Mingliang Sun Kailiang Zhang Baoping Zhang |
| author_facet | Qiaozhen Zhou Qianqian Zhang Lingzi Liao Qian Li Huidan Qu Xinyu Wang Ying Zhou Guangzeng Zhang Mingliang Sun Kailiang Zhang Baoping Zhang |
| author_sort | Qiaozhen Zhou |
| collection | DOAJ |
| description | Isocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechanical properties of ICD in OSCC were determined. Changes in the contents of reactive oxygen species (ROS), the mitochondrial membrane potential (MMP), ATP, and mitochondrial respiratory chain complex enzymes Ⅰ–Ⅳ in cancer cells were studied. ICD significantly inhibited the proliferation of oral tongue squamous cells (Cal-27), with an IC50 of 0.61 mM after 24 h of treatment. The invasion, migration, and adhesion of cancer cells were decreased, and cytoskeletal actin was deformed and depolymerized. In comparison to an untreated group, the activities of mitochondrial respiratory chain complex enzymes I-IV were significantly decreased by 50.72%, 27.39%, 77.27%, and 73.89%, respectively. The ROS production increased, the MMP decreased by 43.65%, and the ATP content decreased to 17.1 ± 0.001 (mmol/mL); ultimately, the apoptosis rate of cancer cells increased up to 10.57% after 24 h of action. These findings suggest that ICD exerted an obvious anticancer activity against OSCC and may inhibit Cal-27 proliferation and growth by causing mitochondrial dysfunction and interrupting cellular energy. |
| first_indexed | 2024-03-08T11:01:40Z |
| format | Article |
| id | doaj.art-f618d166f16b4255bdd64ce14f5810a6 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T11:01:40Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-f618d166f16b4255bdd64ce14f5810a62024-01-26T15:50:52ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-01-0146165066210.3390/cimb46010042Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma CellsQiaozhen Zhou0Qianqian Zhang1Lingzi Liao2Qian Li3Huidan Qu4Xinyu Wang5Ying Zhou6Guangzeng Zhang7Mingliang Sun8Kailiang Zhang9Baoping Zhang10Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, ChinaIsocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechanical properties of ICD in OSCC were determined. Changes in the contents of reactive oxygen species (ROS), the mitochondrial membrane potential (MMP), ATP, and mitochondrial respiratory chain complex enzymes Ⅰ–Ⅳ in cancer cells were studied. ICD significantly inhibited the proliferation of oral tongue squamous cells (Cal-27), with an IC50 of 0.61 mM after 24 h of treatment. The invasion, migration, and adhesion of cancer cells were decreased, and cytoskeletal actin was deformed and depolymerized. In comparison to an untreated group, the activities of mitochondrial respiratory chain complex enzymes I-IV were significantly decreased by 50.72%, 27.39%, 77.27%, and 73.89%, respectively. The ROS production increased, the MMP decreased by 43.65%, and the ATP content decreased to 17.1 ± 0.001 (mmol/mL); ultimately, the apoptosis rate of cancer cells increased up to 10.57% after 24 h of action. These findings suggest that ICD exerted an obvious anticancer activity against OSCC and may inhibit Cal-27 proliferation and growth by causing mitochondrial dysfunction and interrupting cellular energy.https://www.mdpi.com/1467-3045/46/1/42isocorydineoral squamous cell carcinomaanticancer activityenergy metabolism |
| spellingShingle | Qiaozhen Zhou Qianqian Zhang Lingzi Liao Qian Li Huidan Qu Xinyu Wang Ying Zhou Guangzeng Zhang Mingliang Sun Kailiang Zhang Baoping Zhang Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells Current Issues in Molecular Biology isocorydine oral squamous cell carcinoma anticancer activity energy metabolism |
| title | Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells |
| title_full | Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells |
| title_fullStr | Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells |
| title_full_unstemmed | Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells |
| title_short | Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells |
| title_sort | isocorydine exerts anticancer activity by disrupting the energy metabolism and filamentous actin structures of oral squamous carcinoma cells |
| topic | isocorydine oral squamous cell carcinoma anticancer activity energy metabolism |
| url | https://www.mdpi.com/1467-3045/46/1/42 |
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