Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice
Inflammatory bowel disease (IBD) is a chronic disease characterised by repeated relapses and remissions and a high recurrence rate even after symptom resolution. The primary method for IBD diagnosis is endoscopy; however, this method is expensive, invasive, and cumbersome to use serially. Therefore,...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-01-01
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| Series: | Gut Microbiome |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2632289723000178/type/journal_article |
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| author | Yuta Fujiki Takahisa Tanaka Kyosuke Yakabe Natsumi Seki Masahiro Akiyama Ken Uchida Yun-Gi Kim |
| author_facet | Yuta Fujiki Takahisa Tanaka Kyosuke Yakabe Natsumi Seki Masahiro Akiyama Ken Uchida Yun-Gi Kim |
| author_sort | Yuta Fujiki |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is a chronic disease characterised by repeated relapses and remissions and a high recurrence rate even after symptom resolution. The primary method for IBD diagnosis is endoscopy; however, this method is expensive, invasive, and cumbersome to use serially. Therefore, more convenient and non-invasive methods for IBD diagnosis are needed. In this study, we aimed to identify biological gas markers for the development of gut inflammation. Using dextran sulphate sodium (DSS)-induced colitis mouse models, five biological gases were analysed to identify predictive markers for the development of gut inflammation. Additionally, the correlation between the changes in gas composition, gut microbiota, and inflammatory markers was assessed. The hydrogen (H2) level was found to be negatively correlated with the level of lipocalin-2 (LCN2), a gut inflammation biomarker, and weight loss due to DSS-induced colitis. Furthermore, gut microbes belonging to the Rikenellaceae and Akkermansiaceae families were positively correlated with LCN2 levels and weight loss, whereas Tannerellaceae abundance was negatively correlated with LCN2 level and weight loss and positively correlated with H2 levels. This study provides new insights for IBD diagnosis; the H2 levels in biological gases are a potential biomarker for intestinal inflammation, and specific gut microbes are associated with H2 level changes. |
| first_indexed | 2024-03-08T13:17:55Z |
| format | Article |
| id | doaj.art-f61919da31ba4fb786c3e012bba36e32 |
| institution | Directory Open Access Journal |
| issn | 2632-2897 |
| language | English |
| last_indexed | 2024-03-08T13:17:55Z |
| publishDate | 2024-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Gut Microbiome |
| spelling | doaj.art-f61919da31ba4fb786c3e012bba36e322024-01-18T05:46:15ZengCambridge University PressGut Microbiome2632-28972024-01-01510.1017/gmb.2023.17Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in miceYuta Fujiki0Takahisa Tanaka1Kyosuke Yakabe2Natsumi Seki3Masahiro Akiyama4Ken Uchida5https://orcid.org/0000-0001-8307-5232Yun-Gi Kim6https://orcid.org/0000-0003-4251-1848Research Center for Drug Discovery, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, Japan Division of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, JapanDepartment of Materials Engineering, The University of Tokyo, Tokyo 113-8656, JapanResearch Center for Drug Discovery, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, Japan Division of Biochemistry, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, JapanResearch Center for Drug Discovery, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, JapanResearch Center for Drug Discovery, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, JapanDepartment of Materials Engineering, The University of Tokyo, Tokyo 113-8656, JapanResearch Center for Drug Discovery, Faculty of Pharmacy and Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, JapanInflammatory bowel disease (IBD) is a chronic disease characterised by repeated relapses and remissions and a high recurrence rate even after symptom resolution. The primary method for IBD diagnosis is endoscopy; however, this method is expensive, invasive, and cumbersome to use serially. Therefore, more convenient and non-invasive methods for IBD diagnosis are needed. In this study, we aimed to identify biological gas markers for the development of gut inflammation. Using dextran sulphate sodium (DSS)-induced colitis mouse models, five biological gases were analysed to identify predictive markers for the development of gut inflammation. Additionally, the correlation between the changes in gas composition, gut microbiota, and inflammatory markers was assessed. The hydrogen (H2) level was found to be negatively correlated with the level of lipocalin-2 (LCN2), a gut inflammation biomarker, and weight loss due to DSS-induced colitis. Furthermore, gut microbes belonging to the Rikenellaceae and Akkermansiaceae families were positively correlated with LCN2 levels and weight loss, whereas Tannerellaceae abundance was negatively correlated with LCN2 level and weight loss and positively correlated with H2 levels. This study provides new insights for IBD diagnosis; the H2 levels in biological gases are a potential biomarker for intestinal inflammation, and specific gut microbes are associated with H2 level changes.https://www.cambridge.org/core/product/identifier/S2632289723000178/type/journal_articlegut microbiotahydrogen gasexperimental colitisinflammatory bowel diseasebiomarker |
| spellingShingle | Yuta Fujiki Takahisa Tanaka Kyosuke Yakabe Natsumi Seki Masahiro Akiyama Ken Uchida Yun-Gi Kim Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice Gut Microbiome gut microbiota hydrogen gas experimental colitis inflammatory bowel disease biomarker |
| title | Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| title_full | Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| title_fullStr | Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| title_full_unstemmed | Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| title_short | Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| title_sort | hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice |
| topic | gut microbiota hydrogen gas experimental colitis inflammatory bowel disease biomarker |
| url | https://www.cambridge.org/core/product/identifier/S2632289723000178/type/journal_article |
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