Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice
Edible canna rhizomes contain extremely high levels of resistant starch among cereals and potatoes. We previously showed that feeding canna rhizome starch to mice may increase intestinal barrier function and improve the intestinal environment. Here, we investigated the effects of canna starch intake...
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MDPI AG
2024-02-01
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| author | Ayaka Koida Mamoru Tanaka Rina Kosaka Shoei Okuda Shiro Takei Suzuno Ota Sayaka Yokoyama Kaho Miyake Hiroyuki Watanabe |
| author_facet | Ayaka Koida Mamoru Tanaka Rina Kosaka Shoei Okuda Shiro Takei Suzuno Ota Sayaka Yokoyama Kaho Miyake Hiroyuki Watanabe |
| author_sort | Ayaka Koida |
| collection | DOAJ |
| description | Edible canna rhizomes contain extremely high levels of resistant starch among cereals and potatoes. We previously showed that feeding canna rhizome starch to mice may increase intestinal barrier function and improve the intestinal environment. Here, we investigated the effects of canna starch intake in a murine food allergy model. Five-week-old female BALB/c mice were divided into four groups: Control and OVA groups fed on the control diet (AIN-93G) ad libitum and Canna and OVA-Canna groups fed on the canna diet (AIN-93G with 10% replaced with canna starch). The OVA and OVA-Canna groups were sensitized to ovalbumin (OVA), and the anaphylactic response was assessed by measuring body temperature. Body temperature was significantly lower in the OVA group than in the non-sensitized group, but no decrease was observed in the OVA-Canna group. Fecal weight, fecal mucin content, and goblet cells of colorectal tissue were significantly increased in the Canna and OVA-Canna groups compared with those in the Control and OVA groups. Allergen uptake into the liver was also increased in the OVA group and decreased in the OVA-Canna group to the same level as in the non-sensitized group. These results indicate that canna starch supplementation in a murine food allergy model suppresses anaphylactic symptoms by improving the intestinal environment and reducing allergen uptake by increasing intestinal barrier function. |
| first_indexed | 2024-03-07T22:41:10Z |
| format | Article |
| id | doaj.art-f61c9d0e15cb42ee9ac953e8e74e830c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-07T22:41:10Z |
| publishDate | 2024-02-01 |
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| series | Biomolecules |
| spelling | doaj.art-f61c9d0e15cb42ee9ac953e8e74e830c2024-02-23T15:09:23ZengMDPI AGBiomolecules2218-273X2024-02-0114221510.3390/biom14020215Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in MiceAyaka Koida0Mamoru Tanaka1Rina Kosaka2Shoei Okuda3Shiro Takei4Suzuno Ota5Sayaka Yokoyama6Kaho Miyake7Hiroyuki Watanabe8Faculty of Health and Medical Sciences, Aichi Shukutoku University, 2-9 Katahira, Nagakute 480-1197, Aichi, JapanGraduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, JapanGraduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, JapanGraduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, JapanGraduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, JapanFaculty of Health Science, Suzuka University of Medical Science, 1001-1 Kishioka, Suzuka 510-0293, Mie, JapanDepartment of Food and Nutritional Environment, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya 463-8521, Aichi, JapanGraduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Aichi, JapanFaculty of Nutrition, University of Kochi, 2751-1 Ike, Kochi 781-8515, Kochi, JapanEdible canna rhizomes contain extremely high levels of resistant starch among cereals and potatoes. We previously showed that feeding canna rhizome starch to mice may increase intestinal barrier function and improve the intestinal environment. Here, we investigated the effects of canna starch intake in a murine food allergy model. Five-week-old female BALB/c mice were divided into four groups: Control and OVA groups fed on the control diet (AIN-93G) ad libitum and Canna and OVA-Canna groups fed on the canna diet (AIN-93G with 10% replaced with canna starch). The OVA and OVA-Canna groups were sensitized to ovalbumin (OVA), and the anaphylactic response was assessed by measuring body temperature. Body temperature was significantly lower in the OVA group than in the non-sensitized group, but no decrease was observed in the OVA-Canna group. Fecal weight, fecal mucin content, and goblet cells of colorectal tissue were significantly increased in the Canna and OVA-Canna groups compared with those in the Control and OVA groups. Allergen uptake into the liver was also increased in the OVA group and decreased in the OVA-Canna group to the same level as in the non-sensitized group. These results indicate that canna starch supplementation in a murine food allergy model suppresses anaphylactic symptoms by improving the intestinal environment and reducing allergen uptake by increasing intestinal barrier function.https://www.mdpi.com/2218-273X/14/2/215canna starchtype 1 allergyintestinal barrier functionanaphylactic symptommouse |
| spellingShingle | Ayaka Koida Mamoru Tanaka Rina Kosaka Shoei Okuda Shiro Takei Suzuno Ota Sayaka Yokoyama Kaho Miyake Hiroyuki Watanabe Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice Biomolecules canna starch type 1 allergy intestinal barrier function anaphylactic symptom mouse |
| title | Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice |
| title_full | Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice |
| title_fullStr | Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice |
| title_full_unstemmed | Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice |
| title_short | Canna Starch Improves Intestinal Barrier Function, Inhibits Allergen Uptake, and Suppresses Anaphylactic Symptoms in Ovalbumin-Induced Food Allergy in Mice |
| title_sort | canna starch improves intestinal barrier function inhibits allergen uptake and suppresses anaphylactic symptoms in ovalbumin induced food allergy in mice |
| topic | canna starch type 1 allergy intestinal barrier function anaphylactic symptom mouse |
| url | https://www.mdpi.com/2218-273X/14/2/215 |
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