Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells
Epidemiology studies indicate that fruit and vegetable (FV) rich diets are associated with lower risk of Alzheimer's disease (AD), while, the underlying mechanism is not clear. The health promoting effects of diet-derived antioxidants (such as purified vitamin C, β-carotene, flavonoids) were in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-01-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615005733 |
| _version_ | 1819055310838431744 |
|---|---|
| author | Linhong Yuan Jinmeng Liu Jie Zhen Can Cai Sisi Wang Rong Xiao |
| author_facet | Linhong Yuan Jinmeng Liu Jie Zhen Can Cai Sisi Wang Rong Xiao |
| author_sort | Linhong Yuan |
| collection | DOAJ |
| description | Epidemiology studies indicate that fruit and vegetable (FV) rich diets are associated with lower risk of Alzheimer's disease (AD), while, the underlying mechanism is not clear. The health promoting effects of diet-derived antioxidants (such as purified vitamin C, β-carotene, flavonoids) were indicated in dietary intervention experiments, However, the practical biological efficiency of FV consumed through diet could not be simply mimicked by purified antioxidants or their mixture. An in vitro neuron cell (PC12) culture experiment was carried out in the present study to observe the oxidative damage preventing effects of serum collected from FV juice treated rats. Our results showed that serum collected from FV juice treated rats antagonized the oxidative damage caused by H2O2 treatment, and regulated the antioxidant gene expression. FV juice increased antioxidant biomarker levels and antioxidant enzyme activity in rat serum. Our results indicate that multiple mechanisms are involved in the neuron protective effects of FV-rich diet. |
| first_indexed | 2024-12-21T13:05:30Z |
| format | Article |
| id | doaj.art-f61ffcb6926e4a94864f40c66f7d4680 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T13:05:30Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f61ffcb6926e4a94864f40c66f7d46802022-12-21T19:03:03ZengElsevierJournal of Functional Foods1756-46462016-01-0120496505Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cellsLinhong Yuan0Jinmeng Liu1Jie Zhen2Can Cai3Sisi Wang4Rong Xiao5School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, ChinaSchool of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, ChinaSchool of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, ChinaSchool of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, ChinaSchool of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, ChinaSchool of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China; Corresponding author. No.10 Xitoutiao, You An Men, Beijing 100069, China. Tel.: +86 010 83911651; fax: +86 010 83911512.Epidemiology studies indicate that fruit and vegetable (FV) rich diets are associated with lower risk of Alzheimer's disease (AD), while, the underlying mechanism is not clear. The health promoting effects of diet-derived antioxidants (such as purified vitamin C, β-carotene, flavonoids) were indicated in dietary intervention experiments, However, the practical biological efficiency of FV consumed through diet could not be simply mimicked by purified antioxidants or their mixture. An in vitro neuron cell (PC12) culture experiment was carried out in the present study to observe the oxidative damage preventing effects of serum collected from FV juice treated rats. Our results showed that serum collected from FV juice treated rats antagonized the oxidative damage caused by H2O2 treatment, and regulated the antioxidant gene expression. FV juice increased antioxidant biomarker levels and antioxidant enzyme activity in rat serum. Our results indicate that multiple mechanisms are involved in the neuron protective effects of FV-rich diet.http://www.sciencedirect.com/science/article/pii/S1756464615005733DietFruit and vegetableAntioxidantOxidative damagePC12 cell |
| spellingShingle | Linhong Yuan Jinmeng Liu Jie Zhen Can Cai Sisi Wang Rong Xiao Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells Journal of Functional Foods Diet Fruit and vegetable Antioxidant Oxidative damage PC12 cell |
| title | Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells |
| title_full | Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells |
| title_fullStr | Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells |
| title_full_unstemmed | Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells |
| title_short | Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells |
| title_sort | serum collected from fruit and vegetable juice treated rats antagonizing h2o2 induced oxidative damage in pc12 cells |
| topic | Diet Fruit and vegetable Antioxidant Oxidative damage PC12 cell |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615005733 |
| work_keys_str_mv | AT linhongyuan serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells AT jinmengliu serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells AT jiezhen serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells AT cancai serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells AT sisiwang serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells AT rongxiao serumcollectedfromfruitandvegetablejuicetreatedratsantagonizingh2o2inducedoxidativedamageinpc12cells |