Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city

Purpose: The aim is to evaluate the bacterial contamination in dental unit waterlines (DUWLs) before and after the use of the disinfectant 1% (Lysoformin 3000) for its efficacy also assess the awareness about the same among the practicing dental surgeons of Vadodara city. Methodology: A volume of 20...

Full description

Bibliographic Details
Main Authors: Dhaval Bhadra, Nimisha Shah, Parth Patel, Meetkumar Dedania
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2018-01-01
Series:Endodontology
Subjects:
Online Access:http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2018;volume=30;issue=1;spage=62;epage=65;aulast=Bhadra
_version_ 1811207520971128832
author Dhaval Bhadra
Nimisha Shah
Parth Patel
Meetkumar Dedania
author_facet Dhaval Bhadra
Nimisha Shah
Parth Patel
Meetkumar Dedania
author_sort Dhaval Bhadra
collection DOAJ
description Purpose: The aim is to evaluate the bacterial contamination in dental unit waterlines (DUWLs) before and after the use of the disinfectant 1% (Lysoformin 3000) for its efficacy also assess the awareness about the same among the practicing dental surgeons of Vadodara city. Methodology: A volume of 20 ml water samples from the DUWLs from the clinics of the practicing dental surgeons of Vadodara city was collected. And were asked to answer a validated questionnaire in private. Disinfection of the waterlines was performed twice a week for 2 weeks with 1% disinfectant (Lysoformin 3000) and the samples were again collected. Qualitative and quantitative microbiological analysis was performed for both the predisinfected and postdisinfected samples. Results: Predisinfection samples showed a mean colony count of 670.35 colony forming unit (CFU)/ml which was reduced to 63.90 CFU/ml (P < 0.001) postdisinfection. Questionnaire assessment showed that 70% awareness of the participants about DUWL infections and the microorganisms associated. However, only 20% practiced any type of DUWL disinfection. Conclusion: Lysoformin 3000 can be promising to be used a DUWL disinfection solution as an easy to use and readily available solution with great antibacterial properties. However, still more long-term studies are required to check for its efficacy in anaerobic bacteria as well as resistant species of microorganisms.
first_indexed 2024-04-12T04:05:44Z
format Article
id doaj.art-f620f88808ba49839b9f00d225170366
institution Directory Open Access Journal
issn 0970-7212
language English
last_indexed 2024-04-12T04:05:44Z
publishDate 2018-01-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Endodontology
spelling doaj.art-f620f88808ba49839b9f00d2251703662022-12-22T03:48:37ZengWolters Kluwer Medknow PublicationsEndodontology0970-72122018-01-01301626510.4103/endo.endo_47_17Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara cityDhaval BhadraNimisha ShahParth PatelMeetkumar DedaniaPurpose: The aim is to evaluate the bacterial contamination in dental unit waterlines (DUWLs) before and after the use of the disinfectant 1% (Lysoformin 3000) for its efficacy also assess the awareness about the same among the practicing dental surgeons of Vadodara city. Methodology: A volume of 20 ml water samples from the DUWLs from the clinics of the practicing dental surgeons of Vadodara city was collected. And were asked to answer a validated questionnaire in private. Disinfection of the waterlines was performed twice a week for 2 weeks with 1% disinfectant (Lysoformin 3000) and the samples were again collected. Qualitative and quantitative microbiological analysis was performed for both the predisinfected and postdisinfected samples. Results: Predisinfection samples showed a mean colony count of 670.35 colony forming unit (CFU)/ml which was reduced to 63.90 CFU/ml (P < 0.001) postdisinfection. Questionnaire assessment showed that 70% awareness of the participants about DUWL infections and the microorganisms associated. However, only 20% practiced any type of DUWL disinfection. Conclusion: Lysoformin 3000 can be promising to be used a DUWL disinfection solution as an easy to use and readily available solution with great antibacterial properties. However, still more long-term studies are required to check for its efficacy in anaerobic bacteria as well as resistant species of microorganisms.http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2018;volume=30;issue=1;spage=62;epage=65;aulast=Bhadra1% Lysoformin 3000biofilmdental unit waterline
spellingShingle Dhaval Bhadra
Nimisha Shah
Parth Patel
Meetkumar Dedania
Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
Endodontology
1% Lysoformin 3000
biofilm
dental unit waterline
title Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
title_full Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
title_fullStr Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
title_full_unstemmed Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
title_short Evaluation of bacterial contamination of dental unit water lines before and after the use of 1% (Lysoformin 3000) disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of Vadodara city
title_sort evaluation of bacterial contamination of dental unit water lines before and after the use of 1 lysoformin 3000 disinfectant and awareness of the dental unit waterline disinfection among the practicing dental surgeons of vadodara city
topic 1% Lysoformin 3000
biofilm
dental unit waterline
url http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2018;volume=30;issue=1;spage=62;epage=65;aulast=Bhadra
work_keys_str_mv AT dhavalbhadra evaluationofbacterialcontaminationofdentalunitwaterlinesbeforeandaftertheuseof1lysoformin3000disinfectantandawarenessofthedentalunitwaterlinedisinfectionamongthepracticingdentalsurgeonsofvadodaracity
AT nimishashah evaluationofbacterialcontaminationofdentalunitwaterlinesbeforeandaftertheuseof1lysoformin3000disinfectantandawarenessofthedentalunitwaterlinedisinfectionamongthepracticingdentalsurgeonsofvadodaracity
AT parthpatel evaluationofbacterialcontaminationofdentalunitwaterlinesbeforeandaftertheuseof1lysoformin3000disinfectantandawarenessofthedentalunitwaterlinedisinfectionamongthepracticingdentalsurgeonsofvadodaracity
AT meetkumardedania evaluationofbacterialcontaminationofdentalunitwaterlinesbeforeandaftertheuseof1lysoformin3000disinfectantandawarenessofthedentalunitwaterlinedisinfectionamongthepracticingdentalsurgeonsofvadodaracity