Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children
Allergen exposure and rhinovirus (RV) infections are common triggers of acute wheezing exacerbations in early childhood. The identification of such trigger factors is difficult but may have therapeutic implications. Increases of IgE and IgG in sera, were shown against allergens and the N-terminal po...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/5/915 |
| _version_ | 1797533988741447680 |
|---|---|
| author | Katarzyna Niespodziana Katarina Stenberg-Hammar Nikolaos G. Papadopoulos Margarete Focke-Tejkl Peter Errhalt Jon R. Konradsen Cilla Söderhäll Marianne van Hage Gunilla Hedlin Rudolf Valenta |
| author_facet | Katarzyna Niespodziana Katarina Stenberg-Hammar Nikolaos G. Papadopoulos Margarete Focke-Tejkl Peter Errhalt Jon R. Konradsen Cilla Söderhäll Marianne van Hage Gunilla Hedlin Rudolf Valenta |
| author_sort | Katarzyna Niespodziana |
| collection | DOAJ |
| description | Allergen exposure and rhinovirus (RV) infections are common triggers of acute wheezing exacerbations in early childhood. The identification of such trigger factors is difficult but may have therapeutic implications. Increases of IgE and IgG in sera, were shown against allergens and the N-terminal portion of the VP1 proteins of RV species, respectively, several weeks after allergen exposure or RV infection. Hence, increases in VP1-specific IgG and in allergen-specific IgE may serve as biomarkers for RV infections or allergen exposure. The MeDALL-allergen chip containing comprehensive panels of allergens and the PreDicta RV chip equipped with VP1-derived peptides, representative of three genetic RV species, were used to measure allergen-specific IgE levels and RV-species-specific IgG levels in sera obtained from 120 preschool children at the time of an acute wheezing attack and convalescence. Nearly 20% of the children (22/120) showed specific IgE sensitizations to at least one of the allergen molecules on the MeDALL chip. For 87% of the children, increases in RV-specific IgG could be detected in the follow-up sera. This percentage of RV-specific IgG increases was equal in IgE-positive and -negative children. In 10% of the children, increases or <i>de novo</i> appearances of IgE sensitizations indicative of allergen exposure could be detected. Our results suggest that, in the majority of preschool children, RV infections trigger wheezing attacks, but, in addition, allergen exposure seems to play a role as a trigger factor. RV-induced wheezing attacks occur in IgE-sensitized and non-IgE-sensitized children, indicating that allergic sensitization is not a prerequisite for RV-induced wheeze. |
| first_indexed | 2024-03-10T11:23:29Z |
| format | Article |
| id | doaj.art-f62191fd38924329b627f376e67793cc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:23:29Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f62191fd38924329b627f376e67793cc2023-11-21T19:53:12ZengMDPI AGViruses1999-49152021-05-0113591510.3390/v13050915Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool ChildrenKatarzyna Niespodziana0Katarina Stenberg-Hammar1Nikolaos G. Papadopoulos2Margarete Focke-Tejkl3Peter Errhalt4Jon R. Konradsen5Cilla Söderhäll6Marianne van Hage7Gunilla Hedlin8Rudolf Valenta9Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaAstrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 77 Stockholm, SwedenDivision of Infection, Immunity & Respiratory Medicine, University of Manchester, Manchester M13 9NT, UKDivision of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pneumology, University Hospital Krems, Austria, and Karl Landsteiner University of Health Sciences, 3500 Krems, AustriaAstrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 77 Stockholm, SwedenAstrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 77 Stockholm, SwedenDivision of Immunology and Allergy, Department of Medicine, Solna, Karolinska Institutet and Karolinska University Hospital, 171 77 Stockholm, SwedenAstrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 77 Stockholm, SwedenDivision of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaAllergen exposure and rhinovirus (RV) infections are common triggers of acute wheezing exacerbations in early childhood. The identification of such trigger factors is difficult but may have therapeutic implications. Increases of IgE and IgG in sera, were shown against allergens and the N-terminal portion of the VP1 proteins of RV species, respectively, several weeks after allergen exposure or RV infection. Hence, increases in VP1-specific IgG and in allergen-specific IgE may serve as biomarkers for RV infections or allergen exposure. The MeDALL-allergen chip containing comprehensive panels of allergens and the PreDicta RV chip equipped with VP1-derived peptides, representative of three genetic RV species, were used to measure allergen-specific IgE levels and RV-species-specific IgG levels in sera obtained from 120 preschool children at the time of an acute wheezing attack and convalescence. Nearly 20% of the children (22/120) showed specific IgE sensitizations to at least one of the allergen molecules on the MeDALL chip. For 87% of the children, increases in RV-specific IgG could be detected in the follow-up sera. This percentage of RV-specific IgG increases was equal in IgE-positive and -negative children. In 10% of the children, increases or <i>de novo</i> appearances of IgE sensitizations indicative of allergen exposure could be detected. Our results suggest that, in the majority of preschool children, RV infections trigger wheezing attacks, but, in addition, allergen exposure seems to play a role as a trigger factor. RV-induced wheezing attacks occur in IgE-sensitized and non-IgE-sensitized children, indicating that allergic sensitization is not a prerequisite for RV-induced wheeze.https://www.mdpi.com/1999-4915/13/5/915rhinoviruswheezeasthmapreschool childrenallergyallergen |
| spellingShingle | Katarzyna Niespodziana Katarina Stenberg-Hammar Nikolaos G. Papadopoulos Margarete Focke-Tejkl Peter Errhalt Jon R. Konradsen Cilla Söderhäll Marianne van Hage Gunilla Hedlin Rudolf Valenta Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children Viruses rhinovirus wheeze asthma preschool children allergy allergen |
| title | Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children |
| title_full | Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children |
| title_fullStr | Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children |
| title_full_unstemmed | Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children |
| title_short | Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children |
| title_sort | microarray technology may reveal the contribution of allergen exposure and rhinovirus infections as possible triggers for acute wheezing attacks in preschool children |
| topic | rhinovirus wheeze asthma preschool children allergy allergen |
| url | https://www.mdpi.com/1999-4915/13/5/915 |
| work_keys_str_mv | AT katarzynaniespodziana microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT katarinastenberghammar microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT nikolaosgpapadopoulos microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT margaretefocketejkl microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT petererrhalt microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT jonrkonradsen microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT cillasoderhall microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT mariannevanhage microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT gunillahedlin microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren AT rudolfvalenta microarraytechnologymayrevealthecontributionofallergenexposureandrhinovirusinfectionsaspossibletriggersforacutewheezingattacksinpreschoolchildren |