A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken
4,4′-dinitrocarbanilide (DNC) is a key component and marker residue of nicarbazin, which forms residues in edible tissue and then causes nephrotoxicity and hepatotoxicity in humans if used excessively. To simplify sample preparation and monitor the DNC rapidly and accurately, a comparable icELISA an...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/7/628 |
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| author | Qianxin Liang Chen Chen Wenqing Xu Ning Zhang Jielin Yang Wei Song Huimei Cai Ruyan Hou Hongfang Li Xiya Zhang |
| author_facet | Qianxin Liang Chen Chen Wenqing Xu Ning Zhang Jielin Yang Wei Song Huimei Cai Ruyan Hou Hongfang Li Xiya Zhang |
| author_sort | Qianxin Liang |
| collection | DOAJ |
| description | 4,4′-dinitrocarbanilide (DNC) is a key component and marker residue of nicarbazin, which forms residues in edible tissue and then causes nephrotoxicity and hepatotoxicity in humans if used excessively. To simplify sample preparation and monitor the DNC rapidly and accurately, a comparable icELISA and lateral flow immunoassay (LFIA) was developed in this study. Briefly, the reaction parameters were explored for improving the sensitivity of icELISA and LFIA. Under the optimal conditions, methanol was selected as the extracting solvent for DNC in chicken, and 20- and 10-fold dilutions of sample extraction eliminated the matrix effect for icELISA and LFIA, separately. After sample pretreatment, the analysis properties of icELISA and LFIA were compared. The limit of detection of icELISA for DNC was 0.8 μg/kg, and the visual and quantitative limits of detection of LFIA were 8 and 2.5 μg/kg. Compared with icELISA, LFIA showed lower sensitivity but obvious advantages in terms of matrix tolerance and detection time (within 15 min). The sensitivity, specificity, and accuracy of the developed assays satisfied the detection requirement even if using simple sample pretreatment. This comparable icELISA and LFIA provided mutual verifiability methods for the accurate detection of DNC in chicken. |
| first_indexed | 2024-03-11T00:35:44Z |
| format | Article |
| id | doaj.art-f6252f94ea8f4ce19f1f89d4e6936835 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T00:35:44Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-f6252f94ea8f4ce19f1f89d4e69368352023-11-18T21:37:32ZengMDPI AGToxics2305-63042023-07-0111762810.3390/toxics11070628A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in ChickenQianxin Liang0Chen Chen1Wenqing Xu2Ning Zhang3Jielin Yang4Wei Song5Huimei Cai6Ruyan Hou7Hongfang Li8Xiya Zhang9Animal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaHefei Customs District Technical Center, Anhui Key Lab of Analysis and Detection for Food Safety, Hefei 230022, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China4,4′-dinitrocarbanilide (DNC) is a key component and marker residue of nicarbazin, which forms residues in edible tissue and then causes nephrotoxicity and hepatotoxicity in humans if used excessively. To simplify sample preparation and monitor the DNC rapidly and accurately, a comparable icELISA and lateral flow immunoassay (LFIA) was developed in this study. Briefly, the reaction parameters were explored for improving the sensitivity of icELISA and LFIA. Under the optimal conditions, methanol was selected as the extracting solvent for DNC in chicken, and 20- and 10-fold dilutions of sample extraction eliminated the matrix effect for icELISA and LFIA, separately. After sample pretreatment, the analysis properties of icELISA and LFIA were compared. The limit of detection of icELISA for DNC was 0.8 μg/kg, and the visual and quantitative limits of detection of LFIA were 8 and 2.5 μg/kg. Compared with icELISA, LFIA showed lower sensitivity but obvious advantages in terms of matrix tolerance and detection time (within 15 min). The sensitivity, specificity, and accuracy of the developed assays satisfied the detection requirement even if using simple sample pretreatment. This comparable icELISA and LFIA provided mutual verifiability methods for the accurate detection of DNC in chicken.https://www.mdpi.com/2305-6304/11/7/628icELISAlateral flow immunoassay4,4′-dinitrocarbaniliderapid detectionchicken |
| spellingShingle | Qianxin Liang Chen Chen Wenqing Xu Ning Zhang Jielin Yang Wei Song Huimei Cai Ruyan Hou Hongfang Li Xiya Zhang A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken Toxics icELISA lateral flow immunoassay 4,4′-dinitrocarbanilide rapid detection chicken |
| title | A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken |
| title_full | A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken |
| title_fullStr | A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken |
| title_full_unstemmed | A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken |
| title_short | A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4′-Dinitrocarbanilide in Chicken |
| title_sort | comparable icelisa and lateral flow immunoassay for the sensitive and rapid detection of 4 4 dinitrocarbanilide in chicken |
| topic | icELISA lateral flow immunoassay 4,4′-dinitrocarbanilide rapid detection chicken |
| url | https://www.mdpi.com/2305-6304/11/7/628 |
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