Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme
Enzymes/Nanoparticles (NPs) bioconjugates are massively used nowadays to develop thin films for optical and electrochemical biosensors. Nevertheless, their full characterization as a thin coating onto electrodes remains little discussed, in particular the influence of NPs size and enzyme/NPs ratio u...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/10/3309 |
| _version_ | 1797497474766602240 |
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| author | Rémy Savin Christian Blanck Nour-Ouda Benzaamia Fouzia Boulmedais |
| author_facet | Rémy Savin Christian Blanck Nour-Ouda Benzaamia Fouzia Boulmedais |
| author_sort | Rémy Savin |
| collection | DOAJ |
| description | Enzymes/Nanoparticles (NPs) bioconjugates are massively used nowadays to develop thin films for optical and electrochemical biosensors. Nevertheless, their full characterization as a thin coating onto electrodes remains little discussed, in particular the influence of NPs size and enzyme/NPs ratio used in the electrodeposition solution. In this study, GOx (160 kDa) and HRP (44 kDa) were used in association with tannic acid capped gold NPs (a series with sizes from 7 to 40 nm) to electrodeposit biosensor coatings, sensitive towards glucose and H<sub>2</sub>O<sub>2</sub>, respectively. The electrodeposition process was based on a mussel-inspired electro-crosslinking between gallol moieties of tannic acid (at the surface of NPs) and amine moieties of the enzymes. On one hand, the sensitivity of the GOx/NPs coatings depends strongly on the NP size and the enzyme/NPs molar ratio of the electrodeposition solution. An optimal sensitivity was obtained by electrodeposition of 11 nm NPs at a GOx/NPs molar ratio close to the theoretical value of the enzyme monolayer. On the other hand, a modest influence of the NPs size was found on the sensitivity in the case of the electrodeposited HRP/NPs coatings, reaching a plateau at the HRP/NPs molar ratio close to the value of the theoretical enzyme monolayer. In both cases, the enzyme/NPs molar ratio played a role in the sensitivity. To fully understand the parameters driving the biosensor sensitivity, a comprehensive evaluation of the colloidal state of the bioconjugates is proposed here. |
| first_indexed | 2024-03-10T03:18:45Z |
| format | Article |
| id | doaj.art-f6283490b5e047bb9569ca020b3e06db |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:18:45Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f6283490b5e047bb9569ca020b3e06db2023-11-23T12:24:26ZengMDPI AGMolecules1420-30492022-05-012710330910.3390/molecules27103309Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/EnzymeRémy Savin0Christian Blanck1Nour-Ouda Benzaamia2Fouzia Boulmedais3Institut Charles Sadron, University of Strasbourg CNRS, UPR 22, 67034 Strasbourg, FranceInstitut Charles Sadron, University of Strasbourg CNRS, UPR 22, 67034 Strasbourg, FranceInstitut Charles Sadron, University of Strasbourg CNRS, UPR 22, 67034 Strasbourg, FranceInstitut Charles Sadron, University of Strasbourg CNRS, UPR 22, 67034 Strasbourg, FranceEnzymes/Nanoparticles (NPs) bioconjugates are massively used nowadays to develop thin films for optical and electrochemical biosensors. Nevertheless, their full characterization as a thin coating onto electrodes remains little discussed, in particular the influence of NPs size and enzyme/NPs ratio used in the electrodeposition solution. In this study, GOx (160 kDa) and HRP (44 kDa) were used in association with tannic acid capped gold NPs (a series with sizes from 7 to 40 nm) to electrodeposit biosensor coatings, sensitive towards glucose and H<sub>2</sub>O<sub>2</sub>, respectively. The electrodeposition process was based on a mussel-inspired electro-crosslinking between gallol moieties of tannic acid (at the surface of NPs) and amine moieties of the enzymes. On one hand, the sensitivity of the GOx/NPs coatings depends strongly on the NP size and the enzyme/NPs molar ratio of the electrodeposition solution. An optimal sensitivity was obtained by electrodeposition of 11 nm NPs at a GOx/NPs molar ratio close to the theoretical value of the enzyme monolayer. On the other hand, a modest influence of the NPs size was found on the sensitivity in the case of the electrodeposited HRP/NPs coatings, reaching a plateau at the HRP/NPs molar ratio close to the value of the theoretical enzyme monolayer. In both cases, the enzyme/NPs molar ratio played a role in the sensitivity. To fully understand the parameters driving the biosensor sensitivity, a comprehensive evaluation of the colloidal state of the bioconjugates is proposed here.https://www.mdpi.com/1420-3049/27/10/3309bioconjugatesglucose oxidaseperoxidaseelectrodepositionelectropolymerizationcatechol |
| spellingShingle | Rémy Savin Christian Blanck Nour-Ouda Benzaamia Fouzia Boulmedais Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme Molecules bioconjugates glucose oxidase peroxidase electrodeposition electropolymerization catechol |
| title | Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme |
| title_full | Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme |
| title_fullStr | Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme |
| title_full_unstemmed | Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme |
| title_short | Optimization of Nanohybrid Biosensors Based on Electro-Crosslinked Tannic Acid Capped Nanoparticles/Enzyme |
| title_sort | optimization of nanohybrid biosensors based on electro crosslinked tannic acid capped nanoparticles enzyme |
| topic | bioconjugates glucose oxidase peroxidase electrodeposition electropolymerization catechol |
| url | https://www.mdpi.com/1420-3049/27/10/3309 |
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