Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis
Objective(s): Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-10-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_18867_53c0ab5c33f399b321bba16a4af5280a.pdf |
| _version_ | 1819095167082168320 |
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| author | Seyed Masih Etemad aubi Hosein Honari Hamed Bagheri Rohollah Ghasemi Mojtaba Noofeli Seyed Mojtaba Aghaie |
| author_facet | Seyed Masih Etemad aubi Hosein Honari Hamed Bagheri Rohollah Ghasemi Mojtaba Noofeli Seyed Mojtaba Aghaie |
| author_sort | Seyed Masih Etemad aubi |
| collection | DOAJ |
| description | Objective(s): Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods: To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated by cell culture testing.Results: The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion: The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax. |
| first_indexed | 2024-12-21T23:38:59Z |
| format | Article |
| id | doaj.art-f6283f8548364094a1cbd6ae675767e4 |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-21T23:38:59Z |
| publishDate | 2021-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-f6283f8548364094a1cbd6ae675767e42022-12-21T18:46:17ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042021-10-018427027810.22038/nmj.2021.59843.161618867Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracisSeyed Masih Etemad aubi0Hosein Honari1Hamed Bagheri2Rohollah Ghasemi3Mojtaba Noofeli4Seyed Mojtaba Aghaie5Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, IranDepartment of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, IranFaculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, 14115-336, IranDepartment of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-175, IranDepartment of Human Bacterial Vaccines Production & Research, Razi Vaccine and Serum Research Institute, Karaj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, IranDepartment of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, IranObjective(s): Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.Materials and Methods: To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated by cell culture testing.Results: The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.Conclusion: The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.https://nmj.mums.ac.ir/article_18867_53c0ab5c33f399b321bba16a4af5280a.pdfanthraxprotective antigenvaccinempeg-pla |
| spellingShingle | Seyed Masih Etemad aubi Hosein Honari Hamed Bagheri Rohollah Ghasemi Mojtaba Noofeli Seyed Mojtaba Aghaie Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis Nanomedicine Journal anthrax protective antigen vaccine mpeg-pla |
| title | Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis |
| title_full | Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis |
| title_fullStr | Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis |
| title_full_unstemmed | Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis |
| title_short | Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis |
| title_sort | evaluation of mpeg pla nanoparticles as vaccine delivery system for modified protective antigen of bacillus anthracis |
| topic | anthrax protective antigen vaccine mpeg-pla |
| url | https://nmj.mums.ac.ir/article_18867_53c0ab5c33f399b321bba16a4af5280a.pdf |
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