Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response.
DNA damage activates checkpoint controls which block progression of cells through the division cycle. Several different checkpoints exist that control transit at different positions in the cell cycle. A role for checkpoint activation in providing resistance of cells to genotoxic anticancer therapy,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3288045?pdf=render |
| _version_ | 1818611740338814976 |
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| author | Elizabeth Richardson Simon R Stockwell He Li Wynne Aherne Maria Emanuela Cuomo Sibylle Mittnacht |
| author_facet | Elizabeth Richardson Simon R Stockwell He Li Wynne Aherne Maria Emanuela Cuomo Sibylle Mittnacht |
| author_sort | Elizabeth Richardson |
| collection | DOAJ |
| description | DNA damage activates checkpoint controls which block progression of cells through the division cycle. Several different checkpoints exist that control transit at different positions in the cell cycle. A role for checkpoint activation in providing resistance of cells to genotoxic anticancer therapy, including chemotherapy and ionizing radiation, is widely recognized. Although the core molecular functions that execute different damage activated checkpoints are known, the signals that control checkpoint activation are far from understood. We used a kinome-spanning RNA interference screen to delineate signalling required for radiation-mediated retinoblastoma protein activation, the recognized executor of G(1) checkpoint control. Our results corroborate the involvement of the p53 tumour suppressor (TP53) and its downstream targets p21(CIP1/WAF1) but infer lack of involvement of canonical double strand break (DSB) recognition known for its role in activating TP53 in damaged cells. Instead our results predict signalling involving the known TP53 phosphorylating kinase PRPK/TP53RK and the JNK/p38MAPK activating kinase STK4/MST1, both hitherto unrecognised for their contribution to DNA damage G1 checkpoint signalling. Our results further predict a network topology whereby induction of p21(CIP1/WAF1) is required but not sufficient to elicit checkpoint activation. Our experiments document a role of the kinases identified in radiation protection proposing their pharmacological inhibition as a potential strategy to increase radiation sensitivity in proliferating cancer cells. |
| first_indexed | 2024-12-16T15:35:08Z |
| format | Article |
| id | doaj.art-f62956240b124200a8685c8ed5dda4df |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T15:35:08Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f62956240b124200a8685c8ed5dda4df2022-12-21T22:26:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3162710.1371/journal.pone.0031627Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response.Elizabeth RichardsonSimon R StockwellHe LiWynne AherneMaria Emanuela CuomoSibylle MittnachtDNA damage activates checkpoint controls which block progression of cells through the division cycle. Several different checkpoints exist that control transit at different positions in the cell cycle. A role for checkpoint activation in providing resistance of cells to genotoxic anticancer therapy, including chemotherapy and ionizing radiation, is widely recognized. Although the core molecular functions that execute different damage activated checkpoints are known, the signals that control checkpoint activation are far from understood. We used a kinome-spanning RNA interference screen to delineate signalling required for radiation-mediated retinoblastoma protein activation, the recognized executor of G(1) checkpoint control. Our results corroborate the involvement of the p53 tumour suppressor (TP53) and its downstream targets p21(CIP1/WAF1) but infer lack of involvement of canonical double strand break (DSB) recognition known for its role in activating TP53 in damaged cells. Instead our results predict signalling involving the known TP53 phosphorylating kinase PRPK/TP53RK and the JNK/p38MAPK activating kinase STK4/MST1, both hitherto unrecognised for their contribution to DNA damage G1 checkpoint signalling. Our results further predict a network topology whereby induction of p21(CIP1/WAF1) is required but not sufficient to elicit checkpoint activation. Our experiments document a role of the kinases identified in radiation protection proposing their pharmacological inhibition as a potential strategy to increase radiation sensitivity in proliferating cancer cells.http://europepmc.org/articles/PMC3288045?pdf=render |
| spellingShingle | Elizabeth Richardson Simon R Stockwell He Li Wynne Aherne Maria Emanuela Cuomo Sibylle Mittnacht Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. PLoS ONE |
| title | Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. |
| title_full | Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. |
| title_fullStr | Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. |
| title_full_unstemmed | Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. |
| title_short | Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. |
| title_sort | mechanism based screen establishes signalling framework for dna damage associated g1 checkpoint response |
| url | http://europepmc.org/articles/PMC3288045?pdf=render |
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