Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071
The marine-derived fungus <i>Aspergillus</i> <i>fumigatus</i> MF071, isolated from sediment collected from the Bohai Sea, China, yielded two new compounds 19<i>S</i>,20-epoxy-18-oxotryprostatin A (<b>1</b>) and 20-hydroxy-18-oxotryprostatin A (<b>...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/7/352 |
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| author | Jianying Han Miaomiao Liu Ian D. Jenkins Xueting Liu Lixin Zhang Ronald J. Quinn Yunjiang Feng |
| author_facet | Jianying Han Miaomiao Liu Ian D. Jenkins Xueting Liu Lixin Zhang Ronald J. Quinn Yunjiang Feng |
| author_sort | Jianying Han |
| collection | DOAJ |
| description | The marine-derived fungus <i>Aspergillus</i> <i>fumigatus</i> MF071, isolated from sediment collected from the Bohai Sea, China, yielded two new compounds 19<i>S</i>,20-epoxy-18-oxotryprostatin A (<b>1</b>) and 20-hydroxy-18-oxotryprostatin A (<b>2</b>), in addition to 28 known compounds (<b>3</b>–<b>30</b>). The chemical structures were established on the basis of 1D, 2D NMR and HRESIMS spectroscopic data. This is the first report on NMR data of monomethylsulochrin-4-sulphate (<b>4</b>) and pseurotin H (<b>10</b>) as naturally occurring compounds. Compounds <b>15</b>, <b>16</b>, <b>20</b>, <b>23</b>, and <b>30</b> displayed weak antibacterial activity (minimum inhibitory concentration: 100 μg/mL). Compounds <b>18</b> and <b>19</b> exhibited strong activity against <i>S. aureus</i> (minimum inhibitory concentration: 6.25 and 3.13 μg/mL, respectively) and <i>E. coli</i> (minimum inhibitory concentration: 6.25 and 3.13 μg/mL, respectively). A genomic data analysis revealed the putative biosynthetic gene clusters <i>ftm</i> for fumitremorgins, <i>pso</i> for pseurotins, <i>fga</i> for fumigaclavines, and <i>hel</i> for helvolinic acid. These putative biosynthetic gene clusters fundamentally underpinned the enzymatic and mechanistic function study for the biosynthesis of these compounds. The current study reported two new compounds and biosynthetic gene clusters of fumitremorgins, pseurotins, fumigaclavines and helvolinic acid from <i>Aspergillus</i> <i>fumigatus</i> MF071. |
| first_indexed | 2024-03-10T18:40:08Z |
| format | Article |
| id | doaj.art-f629e2bf1bec46839b8b697aeaae6218 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T18:40:08Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f629e2bf1bec46839b8b697aeaae62182023-11-20T05:55:58ZengMDPI AGMarine Drugs1660-33972020-07-0118735210.3390/md18070352Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071Jianying Han0Miaomiao Liu1Ian D. Jenkins2Xueting Liu3Lixin Zhang4Ronald J. Quinn5Yunjiang Feng6Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, AustraliaGriffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, AustraliaGriffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, AustraliaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaGriffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, AustraliaGriffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, AustraliaThe marine-derived fungus <i>Aspergillus</i> <i>fumigatus</i> MF071, isolated from sediment collected from the Bohai Sea, China, yielded two new compounds 19<i>S</i>,20-epoxy-18-oxotryprostatin A (<b>1</b>) and 20-hydroxy-18-oxotryprostatin A (<b>2</b>), in addition to 28 known compounds (<b>3</b>–<b>30</b>). The chemical structures were established on the basis of 1D, 2D NMR and HRESIMS spectroscopic data. This is the first report on NMR data of monomethylsulochrin-4-sulphate (<b>4</b>) and pseurotin H (<b>10</b>) as naturally occurring compounds. Compounds <b>15</b>, <b>16</b>, <b>20</b>, <b>23</b>, and <b>30</b> displayed weak antibacterial activity (minimum inhibitory concentration: 100 μg/mL). Compounds <b>18</b> and <b>19</b> exhibited strong activity against <i>S. aureus</i> (minimum inhibitory concentration: 6.25 and 3.13 μg/mL, respectively) and <i>E. coli</i> (minimum inhibitory concentration: 6.25 and 3.13 μg/mL, respectively). A genomic data analysis revealed the putative biosynthetic gene clusters <i>ftm</i> for fumitremorgins, <i>pso</i> for pseurotins, <i>fga</i> for fumigaclavines, and <i>hel</i> for helvolinic acid. These putative biosynthetic gene clusters fundamentally underpinned the enzymatic and mechanistic function study for the biosynthesis of these compounds. The current study reported two new compounds and biosynthetic gene clusters of fumitremorgins, pseurotins, fumigaclavines and helvolinic acid from <i>Aspergillus</i> <i>fumigatus</i> MF071.https://www.mdpi.com/1660-3397/18/7/352<i>Aspergillus fumigatus</i>genome miningchemical diversityantimicrobial activitybiosynthetic gene clusterprenyltransferase |
| spellingShingle | Jianying Han Miaomiao Liu Ian D. Jenkins Xueting Liu Lixin Zhang Ronald J. Quinn Yunjiang Feng Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 Marine Drugs <i>Aspergillus fumigatus</i> genome mining chemical diversity antimicrobial activity biosynthetic gene cluster prenyltransferase |
| title | Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 |
| title_full | Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 |
| title_fullStr | Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 |
| title_full_unstemmed | Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 |
| title_short | Genome-Inspired Chemical Exploration of Marine Fungus <i>Aspergillus fumigatus</i> MF071 |
| title_sort | genome inspired chemical exploration of marine fungus i aspergillus fumigatus i mf071 |
| topic | <i>Aspergillus fumigatus</i> genome mining chemical diversity antimicrobial activity biosynthetic gene cluster prenyltransferase |
| url | https://www.mdpi.com/1660-3397/18/7/352 |
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