Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction
Glutamate receptors (GLRs) are involved in multiple functions during the plant life cycle through affecting the Ca<sup>2+</sup> concentration. However, GLRs in Brassica species have not yet been reported. In this study, 16 glutamate receptor-like channels (GLR) belonged to two groups wer...
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MDPI AG
2022-11-01
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| author | Liu Yang Yumei Zhao Xiaoyu Wu Yang Zhang Yehan Fu Qiaohong Duan Wei Ma Jiabao Huang |
| author_facet | Liu Yang Yumei Zhao Xiaoyu Wu Yang Zhang Yehan Fu Qiaohong Duan Wei Ma Jiabao Huang |
| author_sort | Liu Yang |
| collection | DOAJ |
| description | Glutamate receptors (GLRs) are involved in multiple functions during the plant life cycle through affecting the Ca<sup>2+</sup> concentration. However, GLRs in Brassica species have not yet been reported. In this study, 16 glutamate receptor-like channels (GLR) belonged to two groups were identified in the <i>Brassica rapa</i> (<i>B. rapa</i>) genome by bioinformatic analysis. Most members contain domains of ANF_receptor, Peripla_BP_6, Lig_chan, SBP_bac_3, and Lig_chan_Glu_bd that are closely related to glutamate receptor channels. This gene family contains many elements associated with drought stress, low temperature stress, methyl jasmonate (MeJA), salicylic acid (SA), and other stress resistance. Gene expression profiles showed that <i>BraGLR</i> genes were expressed in roots, stems, leaves, flowers, and siliques. <i>BraGLR5</i> expression was elevated after drought stress in drought-sensitive plants. <i>BraGLR1</i>, <i>BraGLR8</i>, and <i>BraGLR11</i> expression were significantly upregulated after salt stress. <i>BraGLR3</i> expression is higher in the female sterile-line mutants than in the wild type. The expression levels of <i>BraGLR6</i>, <i>BraGLR9</i>, <i>BraGLR12</i>, and <i>BraGLR13</i> were significantly higher in the male sterile-line mutants than in the wild type. The expression of most <i>BraGLRs</i> increased after self-pollination, with <i>BraGLR9</i> exhibiting the greatest increase. These results suggest that <i>BraGLRs</i> play an important role in abiotic stress tolerance and sexual reproduction. |
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| language | English |
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| spelling | doaj.art-f62cb3a945c54b69917ef8ab59d8b9f62023-11-24T10:42:48ZengMDPI AGCells2073-44092022-11-011123372910.3390/cells11233729Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual ReproductionLiu Yang0Yumei Zhao1Xiaoyu Wu2Yang Zhang3Yehan Fu4Qiaohong Duan5Wei Ma6Jiabao Huang7State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271000, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271000, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture, Hebei Agricultural University, Bao’ding 071000, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271000, ChinaGlutamate receptors (GLRs) are involved in multiple functions during the plant life cycle through affecting the Ca<sup>2+</sup> concentration. However, GLRs in Brassica species have not yet been reported. In this study, 16 glutamate receptor-like channels (GLR) belonged to two groups were identified in the <i>Brassica rapa</i> (<i>B. rapa</i>) genome by bioinformatic analysis. Most members contain domains of ANF_receptor, Peripla_BP_6, Lig_chan, SBP_bac_3, and Lig_chan_Glu_bd that are closely related to glutamate receptor channels. This gene family contains many elements associated with drought stress, low temperature stress, methyl jasmonate (MeJA), salicylic acid (SA), and other stress resistance. Gene expression profiles showed that <i>BraGLR</i> genes were expressed in roots, stems, leaves, flowers, and siliques. <i>BraGLR5</i> expression was elevated after drought stress in drought-sensitive plants. <i>BraGLR1</i>, <i>BraGLR8</i>, and <i>BraGLR11</i> expression were significantly upregulated after salt stress. <i>BraGLR3</i> expression is higher in the female sterile-line mutants than in the wild type. The expression levels of <i>BraGLR6</i>, <i>BraGLR9</i>, <i>BraGLR12</i>, and <i>BraGLR13</i> were significantly higher in the male sterile-line mutants than in the wild type. The expression of most <i>BraGLRs</i> increased after self-pollination, with <i>BraGLR9</i> exhibiting the greatest increase. These results suggest that <i>BraGLRs</i> play an important role in abiotic stress tolerance and sexual reproduction.https://www.mdpi.com/2073-4409/11/23/3729<i>Brassica rapa</i><i>GLR</i> gene familyexpression profileabiotic stresssexual reproduction |
| spellingShingle | Liu Yang Yumei Zhao Xiaoyu Wu Yang Zhang Yehan Fu Qiaohong Duan Wei Ma Jiabao Huang Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction Cells <i>Brassica rapa</i> <i>GLR</i> gene family expression profile abiotic stress sexual reproduction |
| title | Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction |
| title_full | Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction |
| title_fullStr | Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction |
| title_short | Genome-Wide Identification and Expression Analysis of <i>BraGLRs</i> Reveal Their Potential Roles in Abiotic Stress Tolerance and Sexual Reproduction |
| title_sort | genome wide identification and expression analysis of i braglrs i reveal their potential roles in abiotic stress tolerance and sexual reproduction |
| topic | <i>Brassica rapa</i> <i>GLR</i> gene family expression profile abiotic stress sexual reproduction |
| url | https://www.mdpi.com/2073-4409/11/23/3729 |
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