Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals
We report the spatial structure of phosphoribosyl pyrophosphate synthetase 2 from the thermophilic bacterium <i>Thermus thermophilus</i> HB27 (TthPRPPS2) obtained at a 1.85 Å resolution using a diffraction set collected from rhombohedral crystals (space group <i>R3<sub>2</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/11/9/1128 |
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| author | Yulia Abramchik Evgeniy Zayats Maria Kostromina Dmitry Lykoshin Ilya Fateev Irina Konstantinova Nadezda Zhukhlistova Vladimir Timofeev Inna Kuranova Roman Esipov |
| author_facet | Yulia Abramchik Evgeniy Zayats Maria Kostromina Dmitry Lykoshin Ilya Fateev Irina Konstantinova Nadezda Zhukhlistova Vladimir Timofeev Inna Kuranova Roman Esipov |
| author_sort | Yulia Abramchik |
| collection | DOAJ |
| description | We report the spatial structure of phosphoribosyl pyrophosphate synthetase 2 from the thermophilic bacterium <i>Thermus thermophilus</i> HB27 (TthPRPPS2) obtained at a 1.85 Å resolution using a diffraction set collected from rhombohedral crystals (space group <i>R3<sub>2</sub>-h</i>), grown with lithium sulfate as a precipitant. This crystal structure was compared with the structure of TthPRPPS2, previously obtained at a 2.2 Å resolution using diffraction sets from the tetragonal crystals (space group <i>P4<sub>1</sub>2<sub>1</sub>2</i>), grown with ammonium sulfate as a precipitant. The comparison of these structures allows the study of the differences between protein molecules in both crystalline structures, as well as the packaging of enzyme molecules in crystals of both spatial groups. Our results may contribute to the research of the structural basis of catalytic activity and substrate specificity of this enzyme. |
| first_indexed | 2024-03-10T07:46:58Z |
| format | Article |
| id | doaj.art-f62e568b2fdf4309ab75b50993c7b399 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T07:46:58Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-f62e568b2fdf4309ab75b50993c7b3992023-11-22T12:36:19ZengMDPI AGCrystals2073-43522021-09-01119112810.3390/cryst11091128Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal CrystalsYulia Abramchik0Evgeniy Zayats1Maria Kostromina2Dmitry Lykoshin3Ilya Fateev4Irina Konstantinova5Nadezda Zhukhlistova6Vladimir Timofeev7Inna Kuranova8Roman Esipov9Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, RussiaShubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Leninskii Prospect 59, 119333 Moscow, RussiaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Str., 16/10, GSP-7, 117997 Moscow, RussiaWe report the spatial structure of phosphoribosyl pyrophosphate synthetase 2 from the thermophilic bacterium <i>Thermus thermophilus</i> HB27 (TthPRPPS2) obtained at a 1.85 Å resolution using a diffraction set collected from rhombohedral crystals (space group <i>R3<sub>2</sub>-h</i>), grown with lithium sulfate as a precipitant. This crystal structure was compared with the structure of TthPRPPS2, previously obtained at a 2.2 Å resolution using diffraction sets from the tetragonal crystals (space group <i>P4<sub>1</sub>2<sub>1</sub>2</i>), grown with ammonium sulfate as a precipitant. The comparison of these structures allows the study of the differences between protein molecules in both crystalline structures, as well as the packaging of enzyme molecules in crystals of both spatial groups. Our results may contribute to the research of the structural basis of catalytic activity and substrate specificity of this enzyme.https://www.mdpi.com/2073-4352/11/9/1128phosphoribosyl pyrophosphatescrystalline structuremolecule conformationintermolecular contacts |
| spellingShingle | Yulia Abramchik Evgeniy Zayats Maria Kostromina Dmitry Lykoshin Ilya Fateev Irina Konstantinova Nadezda Zhukhlistova Vladimir Timofeev Inna Kuranova Roman Esipov Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals Crystals phosphoribosyl pyrophosphates crystalline structure molecule conformation intermolecular contacts |
| title | Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals |
| title_full | Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals |
| title_fullStr | Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals |
| title_full_unstemmed | Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals |
| title_short | Comparison of Spatial Structures and Packaging of Phosphorybosil Pyrophosphate Synthetase 2 from <i>Thermus thermophilus</i> HB27 in Rhombohedral and Tetragonal Crystals |
| title_sort | comparison of spatial structures and packaging of phosphorybosil pyrophosphate synthetase 2 from i thermus thermophilus i hb27 in rhombohedral and tetragonal crystals |
| topic | phosphoribosyl pyrophosphates crystalline structure molecule conformation intermolecular contacts |
| url | https://www.mdpi.com/2073-4352/11/9/1128 |
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