YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.
MIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349210/?tool=EBI |
| _version_ | 1818383701966323712 |
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| author | Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff |
| author_facet | Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff |
| author_sort | Rainer Schmid |
| collection | DOAJ |
| description | MIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in cartilage has not been defined in detail until now, we aimed to understand the regulation of p54(nrb) transcription in chondrogenesis. We concentrated on the previously described MIA/CD-RAP-dependent regulatory region in the p54(nrb) promoter and characterized the transcriptional regulation of p54(nrb) by MIA/CD-RAP in cartilage. A series of truncated p54(nrb) promoter constructs and mutagenesis analysis revealed that the transcription factor YBX1, which has not been investigated in chondrogenesis thus far, is the mediator of MIA/CD-RAP dependent activation of p54(nrb) transcription. A systematic analysis of genes carrying this binding site in their promoter region revealed further potential MIA/CD-RAP-regulated genes that have been implicated in cartilage differentiation. In summary, we described the effects of MIA/CD-RAP on transcriptional regulation in chondrocytes. Understanding the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. Uncovering new downstream effectors that function via the activation of YBX1 supports the important role of MIA/CD-RAP in these processes. |
| first_indexed | 2024-12-14T03:10:33Z |
| format | Article |
| id | doaj.art-f62ec8949d7f415dac0b05d36361bac0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T03:10:33Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f62ec8949d7f415dac0b05d36361bac02022-12-21T23:19:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8216610.1371/journal.pone.0082166YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.Rainer SchmidKatharina MeyerRainer SpangBirgit SchittekAnja Katrin BosserhoffMIA/CD-RAP is a small, secreted protein involved in cartilage differentiation and melanoma progression. We recently revealed that p54(nrb) acts as a mediator of MIA/CD-RAP action to promote chondrogenesis and the progression of malignant melanoma. As the molecular mechanism of MIA/CD-RAP action in cartilage has not been defined in detail until now, we aimed to understand the regulation of p54(nrb) transcription in chondrogenesis. We concentrated on the previously described MIA/CD-RAP-dependent regulatory region in the p54(nrb) promoter and characterized the transcriptional regulation of p54(nrb) by MIA/CD-RAP in cartilage. A series of truncated p54(nrb) promoter constructs and mutagenesis analysis revealed that the transcription factor YBX1, which has not been investigated in chondrogenesis thus far, is the mediator of MIA/CD-RAP dependent activation of p54(nrb) transcription. A systematic analysis of genes carrying this binding site in their promoter region revealed further potential MIA/CD-RAP-regulated genes that have been implicated in cartilage differentiation. In summary, we described the effects of MIA/CD-RAP on transcriptional regulation in chondrocytes. Understanding the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. Uncovering new downstream effectors that function via the activation of YBX1 supports the important role of MIA/CD-RAP in these processes.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349210/?tool=EBI |
| spellingShingle | Rainer Schmid Katharina Meyer Rainer Spang Birgit Schittek Anja Katrin Bosserhoff YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. PLoS ONE |
| title | YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. |
| title_full | YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. |
| title_fullStr | YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. |
| title_full_unstemmed | YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. |
| title_short | YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis. |
| title_sort | ybx1 is a modulator of mia cd rap dependent chondrogenesis |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349210/?tool=EBI |
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