Molecular diversity and microsatellite polymorphism of modern maize hybrids
The aim of this study was to estimate genetic diversity of 97 modern maize hybrids cultivated in Serbia with 12 microsatellite markers. In total, 89 alleles were detected, with the average of 7.4 alleles per locus. The polymorphic information content ranged from 0.42, for umc1792, to 0.81, for dupss...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2018-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2018/1821-39441802095M.pdf |
| _version_ | 1818282205889167360 |
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| author | Mikić Sanja Brbaklić Ljiljana Stanisavljević Dušan Kondić-Špika Ankica Bekavac Goran Mitrović Bojan Trkulja Dragana Mirosavljević Milan |
| author_facet | Mikić Sanja Brbaklić Ljiljana Stanisavljević Dušan Kondić-Špika Ankica Bekavac Goran Mitrović Bojan Trkulja Dragana Mirosavljević Milan |
| author_sort | Mikić Sanja |
| collection | DOAJ |
| description | The aim of this study was to estimate genetic diversity of 97 modern maize hybrids cultivated in Serbia with 12 microsatellite markers. In total, 89 alleles were detected, with the average of 7.4 alleles per locus. The polymorphic information content ranged from 0.42, for umc1792, to 0.81, for dupssr10, with an average of 0.64. The pairwise relatedness Ritland's values varied between 0.787 and -0.129. Eight markers were sufficient to differentiate hybrids with unique genotypes. A principal coordinate analysis distinguished early and late maturing groups of hybrids, although the observed differentiation between groups was low (Fst = 0.4%). Two markers, bnlg1556 and umc1075, contributed most to the discrimination between early and late genotypes. The values of parameters of molecular genetic diversity were higher in early than in late maturing hybrids, indicating broader genetic basis of the former. A high level of polymorphism of the markers indicates their suitability for fingerprinting. |
| first_indexed | 2024-12-13T00:17:19Z |
| format | Article |
| id | doaj.art-f631dfb1e8da4e98b84d99b4960f5c74 |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-12-13T00:17:19Z |
| publishDate | 2018-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-f631dfb1e8da4e98b84d99b4960f5c742022-12-22T00:05:46ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922018-01-01552951021821-39441802095MMolecular diversity and microsatellite polymorphism of modern maize hybridsMikić Sanja0Brbaklić Ljiljana1Stanisavljević Dušan2Kondić-Špika Ankica3Bekavac Goran4Mitrović Bojan5Trkulja Dragana6Mirosavljević Milan7Institute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadThe aim of this study was to estimate genetic diversity of 97 modern maize hybrids cultivated in Serbia with 12 microsatellite markers. In total, 89 alleles were detected, with the average of 7.4 alleles per locus. The polymorphic information content ranged from 0.42, for umc1792, to 0.81, for dupssr10, with an average of 0.64. The pairwise relatedness Ritland's values varied between 0.787 and -0.129. Eight markers were sufficient to differentiate hybrids with unique genotypes. A principal coordinate analysis distinguished early and late maturing groups of hybrids, although the observed differentiation between groups was low (Fst = 0.4%). Two markers, bnlg1556 and umc1075, contributed most to the discrimination between early and late genotypes. The values of parameters of molecular genetic diversity were higher in early than in late maturing hybrids, indicating broader genetic basis of the former. A high level of polymorphism of the markers indicates their suitability for fingerprinting.https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2018/1821-39441802095M.pdffingerprintinggenetic diversitygenotypinghybridsmaizemarkersmicrosatellitespolymorphisms |
| spellingShingle | Mikić Sanja Brbaklić Ljiljana Stanisavljević Dušan Kondić-Špika Ankica Bekavac Goran Mitrović Bojan Trkulja Dragana Mirosavljević Milan Molecular diversity and microsatellite polymorphism of modern maize hybrids Ratarstvo i Povrtarstvo fingerprinting genetic diversity genotyping hybrids maize markers microsatellites polymorphisms |
| title | Molecular diversity and microsatellite polymorphism of modern maize hybrids |
| title_full | Molecular diversity and microsatellite polymorphism of modern maize hybrids |
| title_fullStr | Molecular diversity and microsatellite polymorphism of modern maize hybrids |
| title_full_unstemmed | Molecular diversity and microsatellite polymorphism of modern maize hybrids |
| title_short | Molecular diversity and microsatellite polymorphism of modern maize hybrids |
| title_sort | molecular diversity and microsatellite polymorphism of modern maize hybrids |
| topic | fingerprinting genetic diversity genotyping hybrids maize markers microsatellites polymorphisms |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2018/1821-39441802095M.pdf |
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